PRC1 Antibody (HRP)

CAT: 0013-GTR18183814Size: 25 µLDry Ice: NoHazardous: No

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CAT#:0013-GTR18183814Size:25 µL

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Description

Carboxypeptidase Y antibody (Peroxidase)

Product Name Alternative

Rabbit anti-Carboxypeptidase Y antibody peroxidase conjugation, HRP conjugated rabbit anti-Carboxypeptidase Y Antibody, Carboxypeptidase YSCY antibody, CPY1 antibody, LBC1 antibody, PRC1 antibody, Vacuolar carboxypeptidase Y antibody, YMR297W antibody, Carboxypeptidase C

UniProt

P00729

Reactivity

Yeast

Immunogen

Carboxypeptidase Y [Baker’s Yeast]

Target

PRC1

Clonality

Polyclonal

Conjugation

HRP

Concentration

1.0 mg/mL

Dilution

ELISA: 1:2,000 - 1:10,000, WB: 1:1,000 - 1:5,000

Purity

Carboxypeptidase Y is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified Carboxypeptidase Y [Baker’s Yeast]. Cross reactivity against Carboxypeptidase Y from other sources may occur but has not been specifically determined.

Form

Liquid (sterile filtered)

Storage Conditions

Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL) . To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Notes

For research use only.

Applications Notes

Anti-Carboxypeptidase Y Peroxidase antibody has been tested by ELISA and western blot and is assayed against 1.0 μg of Carboxypeptidase Y [Baker’s Yeast] in a standard capture ELISA using ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:20,000 to 1:100,000 of the reconstitution concentration is suggested for this product.

Tested Applications

ELISA, WB

NCBI Accession Number

NP_014026.1

Host or Source

Rabbit

Preservative

Preservative: 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Isotype

IgG

UniProtKB · P00729

Carboxypeptidase Y

CBPY_YEAST · Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

View on UniProt ↗

Primary accession: P00729
Review status: UniProtKB reviewed (Swiss-Prot)
Gene: PRC1
Protein existence: 1: Evidence at protein level
Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomy ID: 559292
Alternative names: CPD-Y, cpY
EC number: 3.4.16.5
Processing: Precursor
Secondary accessions: D6W0C4

Protein keywords

Technical term

3D-structureDirect protein sequencingReference proteome

Molecular function

CarboxypeptidaseHydrolaseProtease

PTM

Disulfide bondGlycoproteinZymogen

Domain

Signal

Cellular component

Vacuole

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