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BB-H32, bba64 Antibody

CAT: 0013-GTR18183752Size: 25 µLDry Ice: NoHazardous: No
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CAT#:0013-GTR18183752Size:25 µL
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Description
P35 antibody
Product Name Alternative
Rabbit anti-p35 Antibody, bba64, Borrelia burgdorferi p35
UniProt
O50687
Reactivity
Bacteria
Immunogen
MBP-fusion protein corresponding to Borrelia burgdorferi p35 protein.
Target
BB-H32, bba64
Clonality
Polyclonal
Conjugation
Unconjugated
Concentration
1.4 mg/mL
Dilution
ELISA: >1:5,000, WB: 1:1,000
Purity
This product was Protein-A purified and cross-adsorbed against MBP from monospecific antiserum by chromatography. This antibody is specific for Borrelia burgdorferi p35 protein. A BLAST analysis was used to suggest cross-reactivity with p35 from B. burgdorferi, garinii, and afzelii sources based on 100% homology with the immunizing sequence. Cross-reactivity with p35 from other sources has not been determined.
Form
Liquid (sterile filtered)
Storage Conditions
Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL) . To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
Notes
For research use only.
Applications Notes
This protein-A purified antibody has been tested for use in Western blotting and ELISA. Specific conditions for reactivity should be optimized by the user. Expect a band approximately 27.1 kDa in size corresponding to Borrelia burgdorferi p35 protein by Western blotting in the appropriate cell lysate or extract.
Tested Applications
ELISA, WB
NCBI Accession Number
WP_010256558.1
Host or Source
Rabbit
Preservative
Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Isotype
IgG

UniProtKB · O50687

Protein information

O50687_BORBU · Borreliella burgdorferi (strain ATCC 35210 / DSM 4680 / CIP 102532 / B31)

View on UniProt ↗
Primary accession
O50687
Review status
UniProtKB unreviewed (TrEMBL)
Gene
—
Protein existence
4: Predicted
Organism
Borreliella burgdorferi (strain ATCC 35210 / DSM 4680 / CIP 102532 / B31) (Borrelia burgdorferi)
Taxonomy ID
224326
Alternative names
—
EC number
—
Processing
—
Secondary accessions
—
Protein keywords

PTM

LipoproteinPalmitate

Technical term

PlasmidReference proteome

Domain

Signal

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