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ACHE Antibody (Biotin)

CAT: 0013-GTR18183757Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0013-GTR18183757Size:100 µg
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Description
Acetyl Cholinesterase antibody (Biotin)
Product Name Alternative
Goat anti-Acetyl Cholinesterase Antibody, biotin conjugated goat anti-Acetyl Cholinesterase Antibody, Acetylcholinesterase, AChE
UniProt
P23795
Reactivity
Bovine
Immunogen
Acetyl Cholinesterase [Bovine Erythrocytes]
Target
ACHE
Clonality
Polyclonal
Conjugation
Biotin
Field of Research
Signal Transduction
Concentration
1.0 mg/mL
Dilution
ELISA: 1:2,000 - 1:10,000, IP: 1:100, WB: 1:500 - 1:2,500
Purity
Acetyl Cholinesterase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum as well as purified and partially purified Acetyl Cholinesterase [Bovine Erythrocytes]. Cross reactivity against Acetyl Cholinesterase from other sources is unknown.
Form
Lyophilized
Storage Conditions
Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Notes
For research use only.
Applications Notes
Anti-Acetyl Cholinesterase Biotin Conjugated Antibody has been tested by western blot and is suitable to be assayed against 1.0 μg of Acetyl Cholinesterase in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code as a substrate for 30 minutes at room temperature. A working dilution of 1:1,000 to 1:2,000 is suggested for this product. Reconstitution Buffer: Restore with deionized water (or equivalent) . Reconstitution Volume: 100 μL
Tested Applications
ELISA, IP, WB
NCBI Accession Number
NP_001069688.1
Host or Source
Goat
Preservative
Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Isotype
IgG

UniProtKB · P23795

Acetylcholinesterase

ACES_BOVIN · Bos taurus

View on UniProt ↗
Primary accession
P23795
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
ACHE
Protein existence
1: Evidence at protein level
Organism
Bos taurus (Bovine)
Taxonomy ID
9913
Alternative names
AChE
EC number
3.1.1.7
Processing
Precursor
Secondary accessions
O97579, Q08D79
Protein keywords

Coding sequence diversity

Alternative splicing

Cellular component

Cell membraneMembraneSecretedSynapse

Technical term

Direct protein sequencingReference proteome

PTM

Disulfide bondGlycoproteinGPI-anchorLipoprotein

Molecular function

HydrolaseSerine esterase

Biological process

Neurotransmitter degradation

Domain

Signal

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