Recombinant rabbit anti vimentin

Recombinant rabbit anti vimentin

• Background: Vimentin (57 kDa) is the intermediate filament protein (IFP) of mesenchymal cells. This IFP however often deviates from the tissue-specific and developmentally regulated pattern of expression. Besides its typical expression in most cultured cells, vimentin is also expressed together with several other IFPs during early stages of development. As differentiation proceeds, vimentin is exchanged for the tissue-specific intermediate filament type. Also in cancers, vimentin is often expressed in addition to the tissue-specific IFP.
• CAS Number: 9000-83-3
• UniProt: P08670
• Host: Rabbit
• Species Reactivity: Human, Mouse
• Isotype: IgG
• Clone: RV205
• Type: Monoclonal Antibody
• Source: MUB1904P (clone RV205) is a recombinant rabbit anti vimentin monoclonal antibody produced in mammalian HEK293 cells. _x000D_ Clone RV205 is a reformatted antibody (species switch), produced by cloning the antibody sequence of the mouse anti vimentin hybridoma RV202 (MUB1900P), which was derived by fusion of SP2/0-Ag14 mouse myeloma cells with spleen cells from a BALB/c mouse immunized with a cytoskeletal vimentin extract of calf lens.
• Applications: IHC (frozen and paraffin), ICC, IB
• Field of Research: Cancer, Cardiovascular, Cytoskeleton, Immunology, Neurobiology, Stem cell research
• Assay Principle: MUB1904P (clone RV205) is suitable for immunoblotting, immunocytochemistry, and immunohistochemistry on frozen and paraffin-embedd tissue sections. Optimal antibody dilution should be determined by titration; recommended range is 1:100 – 1:200 for immunohistochemistry with fluorochrome conjµgated secondary antibodies or with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent, and 1:100 – 1:1000 for immunoblotting applications.
• Form: Each vial contains 100 µL 1 mg/mL ProtA purified recombinant monoclonal antibody in PBS containing 0,09% sodium azide_x000D_ The purity of the antibody is >98% as determined by SDS-Polyacrylamide gel electrophoresis._x000D_ The endotoxin level is < 1EU/mg as determined by the LAL chromogenic endotoxin assay.
• Precautions: This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving Humans or animals. This product contains sodium azide. To prevent formation of toxic vapors, do not mix with strong acidic solutions. To prevent formation of potentially explosive metallic azides in metal plumbing, always wash into drain with copious quantities of water. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
• Shipping Conditions: Ambient Temperature
• Storage Conditions: The antibody is shipped at ambient temperature and may be stored at +4°C; For prolonged storage prepare appropriate aliquots and store at or below -20°C; Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2); Repeated thawing and freezing should be avoided; Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day; If a slight precipitation occurs upon storage, this should be removed by centrifugation; It will not affect the performance or the concentration of the product