Biotinylated Mouse CD8 alpha / CD8A Protein, His,Avitag™

Biotinylated Mouse CD8 alpha / CD8A Protein, His,Avitag™

• Background: Integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. Interacts simultaneously with the T-cell receptor (TCR) and the MHC class I proteins presented by antigen presenting cells (APCs). In turn, recruits the Src kinase LCK to the vicinity of the TCR-CD3 complex. In NK-cells, the presence of CD8A homodimers at the cell surface provides a survival mechanism allowing conjugation and lysis of multiple target cells. CD8A homodimer molecules also promote the survival and differentiation of activated lymphocytes into memory CD8 T-cells.
• Description: Biotinylated Mouse CD8 alpha, His,Avitag (CDA-M82E8) is expressed from human 293 cells (HEK293). It contains AA Lys 28 - Tyr 196 (Accession # P01731-1).
• Specifications: This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™). The protein has a calculated MW of 22.5 kDa. The protein migrates as 34-40 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Expression Region: Lys 28 - Tyr 196
• Host: HEK 293
• Target: CD8 alpha
• Conjugation: Biotin-labeled
• Tag: C-10×His & C-Avi
• Source: Mouse
• Stability: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution._x000D_For long term storage, the product should be stored at lyophilized state at -20°C or lower.
• Endotoxin: 1.0 EU per μg
• Purity: 90%
• Format: Powder
• Buffer: PBS, pH7.4
• Additives: Trehalose
• Molecular Weight: 22.5 kDa
• Shipping Conditions: Room Temperature
• Storage Conditions: -20°C