EXCELLYSE Live

• Description:

  The EXCELLYSE Live lysing solution is intended for red blood cell lysis following antibody staining of leukocytes in human peripheral whole blood. This solution is appropriate for use when viable leukocytes are required after red blood cell lysis. The lysing solution is optimized for use with EXBIO single colour monoclonal antibodies and KOMBITEST reagents and can be used in both lyse/wash and lyse/no wash protocol.

• Certification:

  RUO

• Applications:

  FC (QC tested)

• Components:

  The content of the vial (100 mL) is sufficient for 500 Tests. The lysing solution is 10x concentrated and must be diluted 10x with deionized water prior to use (1 volume of concentrated solution with 9 volumes of deionized water) . Always prepare a fresh 1x solution before lysis.

• References & Citations:

  *Alvieri F, Mamani JB, Nucci MP, Oliveira FA, Filgueiras IS, Rego GNA, de Barboza MF, da Silva HR, Gamarra LF: Methods of granulocyte isolation from human blood and labeling with multimodal superparamagnetic iron oxide nanoparticles. Molecules 2020 Feb 11;25 (4) :765., URL: https://pubmed.ncbi.nlm.nih.gov/32053865/, *Pacheco M, Rocha F, Aloia T, Marti L: Evaluation of atypical chemokine receptor expression in T cell subsets. Cells 2022 Dec 16;11 (24) :4099., URL: https://pubmed.ncbi.nlm.nih.gov/36552863/

• Storage Conditions:

  Store at 2-8°C. Do not freeze.