SCAP Antibody (N-term) [AMR09814G]

• Synonyms:

  Sterol regulatory element-binding protein cleavage-activating protein, SCAP, SREBP cleavage-activating protein, SCAP, KIAA0199

• Gene Name:

  SCAP {ECO:0000303|PubMed:10570913, ECO:0000312|HGNC:HGNC:30634}

• Gene ID:

  22937

• UniProt:

  Q12770

• Chromosomal Location:

  Endoplasmic reticulum membrane; Multi-pass membrane protein. Golgi apparatus membrane; Multi-pass membrane protein. Cytoplasmic vesicle, COPII- coated vesicle membrane {ECO:0000250|UniProtKB:P97260}; Multi-pass membrane protein. Note=Moves from the endoplasmic reticulum to the Golgi in the absence of sterols (By similarity) Requires the presence of SPRING1 for proper localization to endoplasmic reticulum (PubMed:32111832) . {ECO:0000250|UniProtKB:P97260, ECO:0000269|PubMed:32111832}

• Applications:

  WB, IHC-P, FC

• Dilution:

  WB--1:1000

• Form:

  Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

• Function:

  Escort protein required for cholesterol as well as lipid homeostasis (By similarity) . Regulates export of the SCAP-SREBP complex from the endoplasmic reticulum to the Golgi upon low cholesterol, thereby regulating the processing of sterol regulatory element-binding proteins (SREBPs) SREBF1/SREBP1 and SREBF2/SREBP2 (By similarity) . At high sterol concentrations, formation of a ternary complex with INSIG (INSIG1 or INSIG2) leads to mask the ER export signal in SCAP, promoting retention of the complex in the endoplasmic reticulum (By similarity) . Low sterol concentrations trigger release of INSIG, a conformational change in the SSD domain of SCAP, unmasking of the ER export signal, promoting recruitment into COPII-coated vesicles and transport of the SCAP-SREBP to the Golgi: in the Golgi, SREBPs are then processed, releasing the transcription factor fragment of SREBPs from the membrane, its import into the nucleus and up-regulation of LDLR, INSIG1 and the mevalonate pathway (By similarity) . Binds cholesterol via its SSD domain (By similarity) .

• Molecular Weight:

  139729

• Storage Conditions:

  Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

• Overview:

  We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality SCAP Antibody (N-term) [AMR09814G].

• Gene ID URL:

  https://www.ncbi.nlm.nih.gov/gene/?term=22937

• Uniprot URL:

  https://www.uniprot.org/uniprot/Q12770