Anti-Hu CD34 PE-Cy™5

• Description:

  CD34 is a highly glycosylated monomeric 111-115 kDa surface protein, which is present on many stem cell populations. It is a well established stem cell marker, though its expression on human hematopoietic stem cells is reversible. CD34 probably serves as a surface receptor that undergoes receptor-mediated endocytosis and regulates adhesion, differentiation and proliferation of hematopoietic stem cells and other progenitors. CD34 expression is likely to represent a specific state of hematopoietic development that may have altered adhering properties with expanding and differentiating capabilities in both in vitro and in vivo conditions.

• Certification:

  RUO

• Negative Species:

  Sheep

• Target Antigen:

  CD34

• Clone:

  581

• Applications:

  FC (QC tested)

• Format:

  PE-Cy™5

• Buffer:

  Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

• References & Citations:

  *Sanz E, Muñ oz-A N, Monserrat J, Van-Den-Rym A, Escoll P, Ranz I, Alvarez-Mon M, de-la-Hera A: Ordering human CD34+CD10-CD19+ pre/pro-B-cell and CD19- common lymphoid progenitor stages in two pro-B-cell development pathways. Proc Natl Acad Sci U S A. 2010 Mar 30;107 (13) :5925-30., URL: http://www.ncbi.nlm.nih.gov/pubmed/20231472 , *Goardon N, Nikolousis E, Sternberg A, Chu WK, Craddock C, Richardson P, Benson R, Drayson M, Standen G, Vyas P, Freeman S: Reduced CD38 expression on CD34+ cells as a diagnostic test in myelodysplastic syndromes. Haematologica. 2009 Aug;94 (8) :1160-3., URL: http://www.ncbi.nlm.nih.gov/pubmed/19644143 , *Iwasaki H, Kawamoto A, Ishikawa M, Oyamada A, Nakamori S, Nishimura H, Sadamoto K, Horii M, Matsumoto T, Murasawa S, Shibata T, Suehiro S, Asahara T: Dose-dependent contribution of CD34-positive cell transplantation to concurrent vasculogenesis and cardiomyogenesis for functional regenerative recovery after myocardial infarction. Circulation. 2006 Mar 14;113 (10) :1311-25., URL: https://www.ncbi.nlm.nih.gov/pubmed/16534028, *Ninos JM, Jefferies LC, Cogle CR, Kerr WG: The thrombopoietin receptor, c-Mpl, is a selective surface marker for human hematopoietic stem cells. J Transl Med. 2006 Feb 16;4:9., URL: https://pubmed.ncbi.nlm.nih.gov/16480521/, *Ono F, Sharma BK, Smith CC, Burnett JW, Aurelian L: CD34+ cells in the peripheral blood transport herpes simplex virus DNA fragments to the skin of patients with erythema multiforme (HAEM) .J Invest Dermatol. 2005 Jun;124 (6) :1215-24., URL: https://pubmed.ncbi.nlm.nih.gov/15955097/, *Suárez L, Vidriales MB, García-Laraña J, Sanz G, Moreno MJ, López A, Barrena S, Martínez R, Tormo M, Palomera L, Lavilla E, López-Berges MC, de Santiago M, de Equiza ME, Miguel JF, Orfao A: CD34+ cells from acute myeloid leukemia, myelodysplastic syndromes, and normal bone marrow display different apoptosis and drug resistance-associated phenotypes. Clin Cancer Res. 2004 Nov 15;10 (22) :7599-606., URL: https://www.ncbi.nlm.nih.gov/pubmed/15569991, *Kato S, Ando K, Nakamura Y, Muguruma Y, Sato T, Yabe H, Yabe M, Hattori K, Yasuda Y, Hotta T: Absence of a CD34- hematopoietic precursor population in recipients of CD34+ stem cell transplantation. Bone Marrow Transplant. 2001 Sep;28 (6) :587-95., URL: https://www.ncbi.nlm.nih.gov/pubmed/11607772, *Felschow DM, McVeigh ML, Hoehn GT, Civin CI, Fackler MJ: The adapter protein CrkL associates with CD34. Blood. 2001 Jun 15;97 (12) :3768-75., URL: https://www.ncbi.nlm.nih.gov/pubmed/11389015, *Janowska-Wieczorek A, Marquez LA, Nabholtz JM, Cabuhat ML, Montaño J, Chang H, Rozmus J, Russell JA, Edwards DR, Turner AR: Growth factors and cytokines upregulate gelatinase expression in bone marrow CD34 (+) cells and their transmigration through reconstituted basement membrane. Blood. 1999 May 15;93 (10) :3379-90., URL: https://www.ncbi.nlm.nih.gov/pubmed/10233890, *Ando K, Nakamura Y, Chargui J, Matsuzawa H, Tsuji T, Kato S, Hotta T: Extensive generation of human cord blood CD34 (+) stem cells from Lin (-) CD34 (-) cells in a long-term in vitro system. Exp Hematol. 2000 Jun;28 (6) :690-9., URL: https://www.ncbi.nlm.nih.gov/pubmed/10880755, *Bonelli P, Nicolussi P, Manetti R, Antuofermo E, Dattena M: Monoclonal antigen do not cross-react with ovine umbilical cord blood cells. Ital J Anim Sci 2010, vol.9:e6., URL: https://www.tandfonline.com/doi/full/10.4081/ijas.2010.e6

• Other References:

  *Krauter J, Hartl M, Hambach L, Kohlenberg A, Gunsilius E, Ganser A, Heil G: Receptor-mediated endocytosis of CD34 on hematopoietic cells after stimulation with the monoclonal antibody anti-HPCA-1. J Hematother Stem Cell Res. 2001 Dec;10 (6) :863-71., URL: http://www.ncbi.nlm.nih.gov/pubmed/11798512?ordina, *Dao MA, Arevalo J, Nolta JA: Reversibility of CD34 expression on human hematopoietic stem cells that retain the capacity for secondary reconstitution. Blood. 2003 Jan 1;101 (1) :112-8., URL: http://www.ncbi.nlm.nih.gov/pubmed/12393633?ordina, *Gangenahalli GU, Singh VK, Verma YK, Gupta P, Sharma RK, Chandra R, Gulati S, Luthra PM: Three-dimensional structure prediction of the interaction of CD34 with the SH3 domain of Crk-L. Stem Cells Dev. 2005 Oct;14 (5) :470-7., URL: http://www.ncbi.nlm.nih.gov/pubmed/16305332?ordina, *Gangenahalli GU, Singh VK, Verma YK, Gupta P, Sharma RK, Chandra R, Luthra PM: Hematopoietic stem cell antigen CD34: role in adhesion or homing. Stem Cells Dev. 2006 Jun;15 (3) :305-13., URL: http://www.ncbi.nlm.nih.gov/pubmed/16846369?ordina

• Storage Conditions:

  Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

• Specificity:

  The mouse monoclonal antibody 581 reacts with an extracellular epitope of CD34, a 110-115 kDa monomeric transmembrane phosphoglycoprotein expressed on hematopoietic progenitors cells and on the most pluripotential stem cells; it is gradually lost on progenitor cells. The antibody recognizes the class III CD34 epitope resistant to neuraminidase, chymopapain and glycoprotease.

• Applications Notes:

  6

• Species:

  Non-human primates, Human

• CAS Number:

  9007-83-4

• Isotype:

  Mouse IgG1 kappa