Anti-Hu CD19 PE-Cy™5

• Description:

  CD19 is a transmembrane glycoprotein of Ig superfamily expressed by B cells from the time of heavy chain rearrangement until plasma cell differentiation. It forms a tetrameric complex with CD21 (complement receptor type 2), CD81 (TAPA-1) and Leu13. Together with BCR (B cell antigen receptor), this complex signals to decrease B cell treshold for activation by the antigen. Besides being signal-amplifying coreceptor for BCR, CD19 can also signal independently of BCR coligation and it turns out to be a central regulatory component upon which multiple signaling pathways converge. Mutation of the CD19 gene results in hypogammaglobulinemia, whereas CD19 overexpression causes B cell hyperactivity.

• Certification:

  RUO

• Immunogen:

  Human CCL (chronic lymphocytic leukemia) cells

• Target Antigen:

  CD19

• Clone:

  4G7

• Applications:

  FC (QC tested)

• Format:

  PE-Cy™5

• Buffer:

  Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

• References & Citations:

  *Muench MO, Roncarolo MG, Namikawa R: Phenotypic and functional evidence for the expression of CD4 by hematopoietic stem cells isolated from human fetal liver. Blood. 1997 Feb 15;89 (4) :1364-75., URL: http://www.ncbi.nlm.nih.gov/pubmed/9028960, *Stockmeyer B, Dechant M, van Egmond M, Tutt AL, Sundarapandiyan K, Graziano RF, Repp R, Kalden JR, Gramatzki M, Glennie MJ, van de Winkel JG, Valerius T: Triggering Fc alpha-receptor I (CD89) recruits neutrophils as effector cells for CD20-directed antibody therapy. J Immunol. 2000 Nov 15;165 (10) :5954-61., URL: http://www.ncbi.nlm.nih.gov/pubmed/11067958 , *Dubois B, Massacrier C, Caux C: Selective attraction of naive and memory B cells by dendritic cells. J Leukoc Biol. 2001 Oct;70 (4) :633-41. , URL: http://www.ncbi.nlm.nih.gov/pubmed/11590201 , *Basu S, Lynne CM, Ruiz P, Aballa TC, Ferrell SM, Brackett NL: Cytofluorographic identification of activated T-cell subpopulations in the semen of men with spinal cord injuries. J Androl. 2002 Jul-Aug;23 (4) :551-6., URL: http://www.ncbi.nlm.nih.gov/pubmed/12065463 , *Köller M, Zwölfer B, Steiner G, Smolen JS, Scheinecker C: Phenotypic and functional deficiencies of monocyte-derived dendritic cells in systemic lupus erythematosus (SLE) patients. Int Immunol. 2004 Nov;16 (11) :1595-604. , URL: http://www.ncbi.nlm.nih.gov/pubmed/15381672 , *Treusch M, Vonthein R, Baur M, Günaydin I, Koch S, Stübiger N, Eckstein AK, Peter HH, Ness T, Zierhut M, Kötter I: Influence of human recombinant interferon-alpha2a (rhIFN-alpha2a) on altered lymphocyte subpopulations and monocytes in Behcet's disease. Rheumatology (Oxford) . 2004 Oct;43 (10) :1275-82., URL: http://www.ncbi.nlm.nih.gov/pubmed/15252211 , *Porcellini S, Vallanti G, Nozza S, Poli G, Lazzarin A, Tambussi G, Siccardi AG, Grassi F: Improved thymopoietic potential in aviremic HIV infected individuals treated with HAART by intermittent IL-2 administration. AIDS. 2003 Jul 25;17 (11) :1621-30., URL: http://www.ncbi.nlm.nih.gov/pubmed/12853744 , *Andersen P, Pedersen MW, Woetmann A, Villingshø j M, Stockhausen MT, Odum N, Poulsen HS: EGFR induces expression of IRF-1 via STAT1 and STAT3 activation leading to growth arrest of human cancer cells. Int J Cancer. 2008 Jan 15;122 (2) :342-9., URL: http://www.ncbi.nlm.nih.gov/pubmed/17918184 , *Martino V, Tonelli R, Montemurro L, Franzoni M, Marino F, Fazzina R, Pession A: Down-regulation of MLL-AF9, MLL and MYC expression is not obligatory for monocyte-macrophage maturation in AML-M5 cell lines carrying t (9;11) (p22; q23) . Oncol Rep. 2006 Jan;15 (1) :207-11., URL: http://www.ncbi.nlm.nih.gov/pubmed/16328057 , *Leukocyte Typing II., Reinherz E.L. et al. (Eds.), Springer-Verlag (1985) ., URL: , *Mueller GA, Smith AM, Chapman MD, Rule GS, Benjamin DC: Hydrogen exchange nuclear magnetic resonance spectroscopy mapping of antibody epitopes on the house dust mite allergen Der p 2. J Biol Chem. 2001 Mar 23;276 (12) :9359-65., URL: http://www.ncbi.nlm.nih.gov/pubmed/11134039, *Smith AM, Benjamin DC, Hozic N, Derewenda U, Smith WA, Thomas WR, Gafvelin G, van Hage-Hamsten M, Chapman MD: The molecular basis of antigenic cross-reactivity between the group 2 mite allergens. J Allergy Clin Immunol. 2001 Jun;107 (6) :977-84., URL: http://www.ncbi.nlm.nih.gov/pubmed/11398074

• Other References:

  *Shi X, Xie C, Chang S, Zhou XJ, Tedder T, Mohan C: CD19 hyperexpression augments Sle1-induced humoral autoimmunity but not clinical nephritis. Arthritis Rheum. 2007 Sep;56 (9) :3057-69., URL: https://pubmed.ncbi.nlm.nih.gov/17763445/, *van Zelm MC, Reisli I, van der Burg M, Castaño D, van Noesel CJ, van Tol MJ, Woellner C, Grimbacher B, Patiño PJ, van Dongen JJ, Franco JL: An antibody-deficiency syndrome due to mutations in the CD19 gene. N Engl J Med. 2006 May 4;354 (18) :1901-12., URL: https://pubmed.ncbi.nlm.nih.gov/16672701/, *Inabe K, Kurosaki T: Tyrosine phosphorylation of B-cell adaptor for phosphoinositide 3-kinase is required for Akt activation in response to CD19 engagement. Blood. 2002 Jan 15;99 (2) :584-9., URL: https://pubmed.ncbi.nlm.nih.gov/11781242/, *Fujimoto M, Poe JC, Jansen PJ, Sato S, Tedder TF: CD19 amplifies B lymphocyte signal transduction by regulating Src-family protein tyrosine kinase activation. J Immunol. 1999 Jun 15;162 (12) :7088-94., URL: https://pubmed.ncbi.nlm.nih.gov/10358152/

• Storage Conditions:

  Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

• Specificity:

  The mouse monoclonal antibody 4G7 recognizes an extracellular epitope of human CD19.

• Applications Notes:

  6

• Species:

  Human

• CAS Number:

  9007-83-4

• Isotype:

  Mouse IgG1 kappa