Anti-Hu CD38 PE-Cy™5

• Description:

  CD38 (NAD+ glycohydrolase) is a type II transmembrane glycoprotein able to induce activation, proliferation and differentiation of mature lymphocytes and mediate apoptosis of myeloid and lymphoid progenitor cells. Another role of CD38 is provided by enzymatic activity of its extracellular part. CD38 acts as NAD+ glycohydrolase converting NAD+ into ADP-ribose, as ADP-ribosyl cyclase producing cADPR and as cADPR hydrolase, thus affecting levels of calcium-mobilizing metabolites. ADPR produced by CD38 serves as an important second messenger of neutrophil and dendritic cell migration. CD38 belongs to markers of B cell subsets, leukemia, and a target of immunotherapy in myeloma treatment.

• Certification:

  RUO

• Immunogen:

  Human thymocytes in foetus

• Target Antigen:

  CD38

• Clone:

  HIT2

• Applications:

  FC (QC tested)

• Format:

  PE-Cy™5

• Buffer:

  Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

• References & Citations:

  *Leukocyte Typing III., McMichael AJ et al (Eds.), Oxford University Press (1987) ., URL: , *Rozková D, Novotná L, Pytlík R, Hochová I, Kozák T, Bartůnková J, Spísek R: Toll-like receptors on B-CLL cells: expression and functional consequences of their stimulation. Int J Cancer. 2010 Mar 1;126 (5) :1132-43., URL: https://www.ncbi.nlm.nih.gov/pubmed/19685493, *Kolar GR, Mehta D, Pelayo R, Capra JD: A novel human B cell subpopulation representing the initial germinal center population to express AID. Blood. 2007 Mar 15;109 (6) :2545-52., URL: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1852199/, *Všianská P, Říhová L, Varmužová T, Suská R, Kryukov F, Mikulášová A, Kupská R, Penka M, Pour L, Adam Z, Hájek R: Analysis of B-cell subpopulations in monoclonal gammopathies. Clin Lymphoma Myeloma Leuk. 2015 Apr;15 (4) :e61-71., URL: http://www.ncbi.nlm.nih.gov/pubmed/25578543 , *Ratuszny D, Skripuletz T, Wegner F, Groß M, Falk C, Jacobs R, Ruschulte H, Stangel M, Sühs KW: Case report: Daratumumab in a patient with severe refractory anti-NMDA receptor encephalitis. Front Neurol. 2020 Dec 22;11:602102., URL: https://pubmed.ncbi.nlm.nih.gov/33414761/, *Pape LJ, Hambach J, Gebhardt AJ, Rissiek B, Stähler T, Tode N, Khan C, Weisel K, Adam G, Koch-Nolte F, Bannas P: CD38-specific nanobodies allow in vivo imaging of multiple myeloma under daratumumab therapy. Front Immunol. 2022 Oct 27:13:1010270., URL: https://pubmed.ncbi.nlm.nih.gov/36389758/, *Taussig DC, Miraki-Moud F, Anjos-Afonso F, Pearce DJ, Allen K, Ridler C, Lillington D, Oakervee H, Cavenagh J, Agrawal SG, Lister TA, Gribben JG, Bonnet D: Anti-CD38 antibody-mediated clearance of human repopulating cells masks the heterogeneity of leukemia-initiating cells. Blood 2008 Aug 1;112 (3) :568-75., URL: https://pubmed.ncbi.nlm.nih.gov/18523148/, *Ruiz-Lorente I, Gimeno L, López-Abad A, López Cubillana P, Fernández Aparicio T, Asensio Egea LJ, Moreno Avilés J, Doñate Iñiguez G, Guzmán Martínez-Valls PL, Server G, Ferri B, Campillo JA, Martínez-Sánchez MV, Minguela A: Differential role of NKG2A/HLA-E interaction in the outcomes of bladder cancer patients treated with M. bovis BCG or other therapies. Biomedicines 2025 Jan 10;13 (1) :156., URL: https://pubmed.ncbi.nlm.nih.gov/39857739/, *Kužílková D, Puñet-Ortiz J, Aui PM, Fernández J, Fišer K, Engel P, van Zelm MC, Kalina T: Standardization of workflow and flow cytometry panels for quantitative expression profiling of surface antigens on blood leukocyte subsets: An HCDM CDMaps initiative. Front Immunol. 2022 Feb 11:13:827898. , URL: https://pubmed.ncbi.nlm.nih.gov/35222411/

• Other References:

  *Cakir-Kiefer C, Muller-Steffner H, Oppenheimer N, Schuber F: Kinetic competence of the cADP-ribose-CD38 complex as an intermediate in the CD38/NAD+ glycohydrolase-catalysed reactions: implication for CD38 signalling. Biochem J. 2001 Sep 1;358 (Pt 2) :399-406., URL: https://www.ncbi.nlm.nih.gov/pubmed/11513738?ordin, *Lund FE, Muller-Steffner H, Romero-Ramirez H, Moreno-García ME, Partida-Sánchez S, Makris M, Oppenheimer NJ, Santos-Argumedo L, Schuber F: CD38 induces apoptosis of a murine pro-B leukemic cell line by a tyrosine kinase-dependent but ADP-ribosyl cyclase- and NAD glycohydrolase-independent mechanism. Int Immunol. 2006 Jul;18 (7) :1029-42., URL: https://www.ncbi.nlm.nih.gov/pubmed/16720618?ordin, *Partida-Sanchez S, Gasser A, Fliegert R, Siebrands CC, Dammermann W, Shi G, Mousseau BJ, Sumoza-Toledo A, Bhagat H, Walseth TF, Guse AH, Lund FE. Chemotaxis of mouse bone marrow neutrophils and dendritic cells is controlled by adp-ribose, the major product generated by the CD38 enzyme reaction. J Immunol. 2007 Dec 1;179 (11) :7827-39., URL: https://www.ncbi.nlm.nih.gov/pubmed/18025229?ordin

• Storage Conditions:

  Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

• Specificity:

  The mouse monoclonal antibody HIT2 reacts with an extracellular epitope of CD38, a 45 kDa type II transmembrane glycoprotein strongly expressed mainly on plasma cells and activated T and B lymphocytes; it is an antigenic marker of lymphoid cells. Its binding is blocked by daratumumab.

• Applications Notes:

  6

• Species:

  Human

• CAS Number:

  9007-83-4

• Isotype:

  Mouse IgG1