Anti-Hu CD31 PE-Cy™5

• Description:

  CD31 (platelet endothelial cell adhesion molecule-1, PECAM-1) is an inhibitory coreceptor involved in regulation of T cell and B cell signaling by a dual immunoreceptor tyrosine-based inhibitory motif (ITIM) that upon associated kinases-mediated phosphorylation provide docking sites for protein-tyrosine phosphatases. CD31 is expressed ubiquitously within the vascular compartment and is located mainly at junctions between adjacent cells. N-terminal Ig-like domain of CD31 is responsible for its homophilic binding, which plays an important role in cell-cell interactions. CD31 is a multifunctional molecule with diverse roles in modulation of integrin-mediated cell adhesion, transendothelial migration, angiogenesis, apoptosis, negative regulation of immunoreceptor signaling, autoimmunity, macrophage phagocytosis, IgE-mediated anaphylaxis and thrombosis. It is one of key regulatory molecules in vascular system.

• Certification:

  RUO

• Immunogen:

  Leukocytes of a patient suffering from LGL-type leukaemia

• Target Antigen:

  CD31

• Clone:

  MEM-05

• Applications:

  FC (QC tested)

• Format:

  PE-Cy™5

• Buffer:

  Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

• References & Citations:

  *Prager E, Staffler G, Majdic O, Saemann M, Godar S, Zlabinger G, Stockinger H: Induction of hyporesponsiveness and impaired T lymphocyte activation by the CD31 receptor:ligand pathway in T cells. J Immunol. 2001 Feb 15;166 (4) :2364-71. , URL: https://www.ncbi.nlm.nih.gov/pubmed/11160294?dopt=, *Cârţână T, Săftoiu A, Gruionu LG, Gheonea DI, Pirici D, Georgescu CV, Ciocâlteu A, Gruionu G: Confocal laser endomicroscopy for the morphometric evaluation of microvessels in human colorectal cancer using targeted anti-CD31 antibodies. PLoS One. 2012;7 (12) :e52815. doi: 10.1371/journal.pone.0052815., URL: https://pubmed.ncbi.nlm.nih.gov/23285192/, *Kalina T, Bakardjieva M, Blom M, Perez-Andres M, Barendregt B, Kanderová V, Bonroy C, Philippé J, Blanco E, Pico-Knijnenburg I, Paping JH, Wolska-Kuśnierz B, Pac M, Tkazcyk J, Haerynck F, Akar HH, Formánková R, Freiberger T, Svatoň M, Šedivá A, Arriba-Méndez S, Orfao A, van Dongen JJ, van der Burg M: EuroFlow standardized approach to diagnostic immunopheneotyping of severe PID innNewborns and young children. Front Immunol. 2020 Mar 19;11:371., URL: https://pubmed.ncbi.nlm.nih.gov/32265901/, *Wei F, Cao S, Ren X, Liu H, Yu J, Li H, Hao X: Efficient antiproliferative and antiangiogenic effects on human ovarian cancer growth by gene transfer of attenuated mutants of Shiga-like toxin I. Int J Gynecol Cancer. Jul-Aug 2008;18 (4) :677-91., URL: https://pubmed.ncbi.nlm.nih.gov/17944920/, *Kužílková D, Puñet-Ortiz J, Aui PM, Fernández J, Fišer K, Engel P, van Zelm MC, Kalina T: Standardization of workflow and flow cytometry panels for quantitative expression profiling of surface antigens on blood leukocyte subsets: An HCDM CDMaps initiative. Front Immunol. 2022 Feb 11:13:827898. , URL: https://pubmed.ncbi.nlm.nih.gov/35222411/, *Szulcek R, Happé CM, Rol N, Fontijn RD, Dickhoff C, Hartemink KJ, Grünberg K, Tu L, Timens W, Nossent GD, Paul MA, Leyen TA, Horrevoets AJ, de Man FS, Guignabert C, Yu PB, Vonk-Noordegraaf A, van Nieuw Amerongen GP, Bogaard HJ: Delayed microvascular shear adaptation in pulmonary arterial hypertension. Role of platelet endothelial cell adhesion molecule-1 cleavage. Am J Respir Crit Care Med. 2016 Jun 15;193 (12) :1410-20., URL: https://pubmed.ncbi.nlm.nih.gov/26760925/

• Other References:

  *Wong MX, Hayball JD, Jackson DE: PECAM-1-regulated signalling thresholds control tolerance in anergic transgenic B-cells. Mol Immunol. 2007 Oct 29, URL: https://www.ncbi.nlm.nih.gov/pubmed/17977600?ordin, *Woodfin A, Voisin MB, Nourshargh S: PECAM-1: a multi-functional molecule in inflammation and vascular biology. Arterioscler Thromb Vasc Biol. 2007 Dec;27 (12) :2514-23., URL: https://www.ncbi.nlm.nih.gov/pubmed/17872453?ordin, *Wee JL, Jackson DE: The Ig-ITIM superfamily member PECAM-1 regulates the "outside-in" signaling properties of integrin alpha (IIb) beta3 in platelets. Blood. 2005 Dec 1;106 (12) :3816-23., URL: https://www.ncbi.nlm.nih.gov/pubmed/16081692?ordin, *Jackson DE: The unfolding tale of PECAM-1. FEBS Lett. 2003 Apr 10;540 (1-3) :7-14., URL: https://www.ncbi.nlm.nih.gov/pubmed/12681475?ordin, *Wilkinson R, Lyons AB, Roberts D, Wong MX, Bartley PA, Jackson DE: Platelet endothelial cell adhesion molecule-1 (PECAM-1/CD31) acts as a regulator of B-cell development, B-cell antigen receptor (BCR) -mediated activation, and autoimmune disease. Blood. 2002 Jul 1;100 (1) :184-93., URL: https://www.ncbi.nlm.nih.gov/pubmed/12070026?ordin, *Newman DK, Hamilton C, Newman PJ: Inhibition of antigen-receptor signaling by Platelet Endothelial Cell Adhesion Molecule-1 (CD31) requires functional ITIMs, SHP-2, and p56 (lck) . Blood. 2001 Apr 15;97 (8) :2351-7., URL: https://www.ncbi.nlm.nih.gov/pubmed/11290597?ordin

• Storage Conditions:

  Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

• Specificity:

  The antibody MEM-05 reacts with an extracellular epitope of CD31 (PECAM-1), a 130-140 kDa type I transmembrane glycoprotein expressed on monocytes, platelets, granulocytes, endothelial cells and stem cells of the myeloid lineage.

• Applications Notes:

  6

• Species:

  Human

• CAS Number:

  9007-83-4

• Isotype:

  Mouse IgG1