Anti-Hu CD55 PE-Cy™5

• Description:

  CD55 (decay-accelerating factor, DAF) is a GPI-anchored membrane glycoprotein that protects autologous cells from classical and alternative pathway of complement cascade. Bidirectional interactions between CD55 and CD97 are involved in T cell regulation and CD55 can still regulate complement when bound to CD97. In tumours, besides protection agains complement, CD55 promotes neoangiogenesis, tumorigenesis, invasiveness and evasion of apoptosis.

• Certification:

  RUO

• Immunogen:

  HPB-ALL human T cell line

• Target Antigen:

  CD55

• Clone:

  MEM-118

• Applications:

  FC (QC tested)

• Format:

  PE-Cy™5

• Buffer:

  Stabilizing Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide

• References & Citations:

  *Angelisová P, Drbal K, Horejsí V, Cerný J: Association of CD10/neutral endopeptidase 24.11 with membrane microdomains rich in glycosylphosphatidylinositol-anchored proteins and Lyn kinase. Blood. 1999 Feb 15;93 (4) :1437-9., URL: http://www.ncbi.nlm.nih.gov/pubmed/10075459?itool=

• Other References:

  *Miwa T, Maldonado MA, Zhou L, Sun X, Luo HY, Cai D, Werth VP, Madaio MP, Eisenberg RA, Song WC: Deletion of decay-accelerating factor (CD55) exacerbates autoimmune disease development in MRL/lpr mice. Am J Pathol. 2002 Sep;161 (3) :1077-86., URL: https://www.ncbi.nlm.nih.gov/pubmed/12213736?ordin, *Mikesch JH, Buerger H, Simon R, Brandt B: Decay-accelerating factor (CD55) : a versatile acting molecule in human malignancies. Biochim Biophys Acta. 2006 Aug;1766 (1) :42-52., URL: https://www.ncbi.nlm.nih.gov/pubmed/16784816?ordin, *Abbott RJ, Spendlove I, Roversi P, Fitzgibbon H, Knott V, Teriete P, McDonnell JM, Handford PA, Lea SM: Structural and functional characterization of a novel T cell receptor co-regulatory protein complex, CD97-CD55. J Biol Chem. 2007 Jul 27;282 (30) :22023-32., URL: https://www.ncbi.nlm.nih.gov/pubmed/17449467?ordin, *Miwa T, Maldonado MA, Zhou L, Yamada K, Gilkeson GS, Eisenberg RA, Song WC: Decay-accelerating factor ameliorates systemic autoimmune disease in MRL/lpr mice via both complement-dependent and -independent mechanisms. Am J Pathol. 2007 Apr;170 (4) :1258-66., URL: https://www.ncbi.nlm.nih.gov/pubmed/17392165?ordin, *VanLandingham JW, Cekic M, Cutler S, Hoffman SW, Stein DG: Neurosteroids reduce inflammation after TBI through CD55 induction. Neurosci Lett. 2007 Sep 25;425 (2) :94-8., URL: https://www.ncbi.nlm.nih.gov/pubmed/17826908?ordin

• Storage Conditions:

  Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

• Specificity:

  The antibody MEM-118 recognizes an epitope in SCR4 domain of CD55 (Decay accelerating factor, DAF), a 60-70 kDa glycosylphosphatidylinositol (GPI) -anchored single chain extracellular glycoprotein. CD55 is widely expressed on hematopoietic and on many non-hematopoietic cells; it is weakly present on NK cells.

• Applications Notes:

  6

• Species:

  Human, Non-human primates

• CAS Number:

  9007-83-4

• Isotype:

  Mouse IgM