Anti-Hu CD4 PE-Cy1537

Availability: 24/48H Stock Items & 2 to 6 Weeks non Stock Items.
Dry Ice Shipment: No

Alternative Products

Cat	Name
MBS1800008-02	Anti-Hu CD64 PE
MBS1800035-02	Anti-Hu CD7 PE
MBS1800052-01	Anti-Hu CD206 PE
MBS1800062-01	Anti-Hu CD140a PE
MBS1800065-01	Anti-Hu CD85g PE
MBS1800071-02	Anti-Hu CD267 PE
MBS1800080-02	Anti-Hu CD314 PE
MBS1800094-01	Anti-Hu CD154 PE
MBS1800097-01	Anti-Hu CD340 PE
MBS1800117-02	Anti-Hu CD45 PE

CAT:

270-T7-359-T100

Size:

100 Tests

Price:

Ask

For price, please contact [email protected].

Anti-Hu CD4 PE-Cy1537

Background: CD4 (T4) is a single chain transmembrane glycoprotein and belongs to immunoglobulin supergene family. In extracellular region there are 4 immunoglobulin-like domains (1 Ig-like V-type and 3 Ig-like C2-type). Transmembrane region forms 25 aa, cytoplasmic tail consists of 38 aa. Domains 1,2 and 4 are stabilized by disulfide bonds. The intracellular domain of CD4 is associated with p56Lck, a Src-like protein tyrosine kinase. It was described that CD4 segregates into specific detergent-resistant T-cell membrane microdomains. Extracellular ligands: MHC class II molecules (binds to CDR2-like region in CD4 domain 1) HIV envelope protein gp120 (binds to CDR2-like region in CD4 domain 1) IL-16 (binds to CD4 domain 3), human seminal plasma glycoprotein gp17 (binds to CD4 domain 1), L-selectin. Intracellular ligands: p56LckCD4 is a co-receptor involved in immune response (co-receptor activity in binding to MHC class II molecules) and HIV infection (human immunodeficiency virus CD4 is primary receptor for HIV-1 surface glycoprotein gp120). CD4 regulates T-cell activation, T/B-cell adhesion, T-cell diferentiation, T-cell selection and signal transduction. Defects in antigen presentation (MHC class II) cause dysfunction of CD4+ T-cells and their almost complete absence in patients blood, tissue and organs (SCID immunodeficiency).
Specifications: The antibody MEM-241 recognizes an extracellular epitope of CD4 antigen, a 55 kDa transmebrane glycoprotein expressed on a subset of T lymphocytes ( 8220helper 8220 T-cells) and also on monocytes, tissue macrophages and granulocytes.
Certification: RUO
Host: Mouse
Species Reactivity: Human
Immunogen: 2 N-terminal domains of human CD4 fused to human IgG1 Fc
Target Antigen: CD4
Isotype: IgG1
Clone: MEM-241
Applications: Flow cytometry: The reagent is designed for analysis of human blood cells using 4 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (0.4 mL) is sufficient for 100 tests.
Validated Applications: FC
Format: PE-Cy1537
Buffer: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
References & Citations: *Brdickova N. et al.: LIME: a new membrane Raft-associated adaptor protein involved in CD4 and CD8 coreceptor signaling. J Exp Med. 2003 Nov 17198(10):1453-62. , URL: https://www.ncbi.nlm.nih.gov/pubmed/14610046?dopt=,*Karlsson KR, Cowley S, Martinez FO, Shaw M, Minger SL, James W: Homogeneous monocytes and macrophages from human embryonic stem cells following coculture-free differentiation in M-CSF and IL-3. Exp Hematol. 2008 Sep36(9):1167-75., URL: https://www.ncbi.nlm.nih.gov/pubmed/18550257?itool,*Manasa J, Musabaike H, Masimirembwa C, Burke E, Luthy R, Mudzori J: Evaluation of the Partec flow cytometer against the BD FACSCalibur system for monitoring immune responses of human immunodeficiency virus-infected patients in Zimbabwe. Clin Vaccine Immunol. 2007 Mar14(3):293-8., URL: https://www.ncbi.nlm.nih.gov/pubmed/17267593?itool,*Anderson AE, Sayers BL, Haniffa MA, Swan DJ, Diboll J, Wang XN, Isaacs JD, Hilkens CM: Differential regulation of naïve and memory CD4+ T cells by alternatively activated dendritic cells. J Leukoc Biol. 2008 Jul84(1):124-33., URL: https://www.ncbi.nlm.nih.gov/pubmed/18430785?itool,*Hovden AO, Karlsen M, Jonsson R, Aarstad HJ, Appel S: Maturation of monocyte derived dendritic cells with OK432 boosts IL-12p70 secretion and conveys strong T-cell responses. BMC Immunol. 2011 Jan 512:2., URL: https://www.ncbi.nlm.nih.gov/pubmed/21208424,*Zola H, Swart B, Banham A, Barry S, Beare A, Bensussan A, Boumsell L, D Buckley C, Buhring HJ, Clark G, Engel P, Fox D, Jin BQ, Macardle PJ, Malavasi F, Mason D, Stockinger H, Yang X: CD molecules 2006--human cell differentiation molecules. J Immunol Methods. 2007 Jan 30319(1-2):1-5. , URL: https://www.ncbi.nlm.nih.gov/pubmed/17174972?dopt=,*Kanderova V, Kuzilkova D, Stuchly J, Vaskova M, Brdicka T, Fiser K, Hrusak O, Lund-Johansen F, Kalina T: High-resolution Antibody Array Analysis of Childhood Acute Leukemia Cells. Mol Cell Proteomics. 2016 Apr15(4):1246-61., URL: http://www.ncbi.nlm.nih.gov/pubmed/26785729 ,*Ohradanova-Repic A, Machacek C, Charvet C, Lager F, Le Roux D, Platzer R, Leksa V, Mitulovic G, Burkard TR, Zlabinger GJ, Fischer MB, Feuillet V, Renault G, Blüml S, Benko M, Suchanek M, Huppa JB, Matsuyama T, Cavaco-Paulo A, Bismuth G, Stockinger H: Extracellular Purine Metabolism Is the Switchboard of Immunosuppressive Macrophages and a Novel Target to Treat Diseases With Macrophage Imbalances. Front Immunol. 2018 Apr 279:852., URL: https://www.ncbi.nlm.nih.gov/pubmed/29780382 ,*Černý V, Novotná O, Petrásková P Hudcová K, Boráková K, Prokešová L, Kolářová L, Hrdý J: Lower functional and proportional characteristics of cord blood treg of male newborns compared with female newborns. Biomedicines 2021, 9, 170., URL: https://pubmed.ncbi.nlm.nih.gov/33572097/,
Other References: *Millan J, Cerny J, Horejsi V, Alonso MA: CD4 segregates into specific detergent-resistant T-cell membrane microdomains. Tissue Antigens. 1999 Jan53(1):33-40., URL: https://pubmed.ncbi.nlm.nih.gov/10082429/,
Storage Conditions: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

Complementary Product Range

Our range of products, including Anti-Hu CD4 PE-Cy1537, falls within the same field of application and can be used across various research projects. These products are carefully selected for their high performance and reliability, supporting the diverse needs of modern laboratories. Whether for ongoing studies or future experiments, they are designed to help researchers achieve their scientific goals. Here are some related products that may be of interest for similar or future applications:

Each item in our collection, is tested for precision and quality, ensuring dependable solutions for your research. As we continue to incorporate the latest advancements, Gentaur ensures that our product range remains relevant and versatile, providing you with the tools needed for both current and future projects.

Find us on

Cat	Name
T7-208-T100	Anti-Hu CD9 PE-Cy1537
T7-503-T100	Anti-Hu CD24 PE-Cy1537
T7-676-T100	Anti-Hu CD79b PE-Cy1537
T7-146-T100	Anti-Hu CD184 PE-Cy1537
T7-173-T025	Anti-Hu CD3 PE-Cy1537
T7-178-T100	Anti-Hu CD273 PE-Cy1537
T7-218-T025	Anti-Hu CD25 PE-Cy1537
T7-287-T025	Anti-Hu CD80 PE-Cy1537
T7-287-T100	Anti-Hu CD80 PE-Cy1537
T7-305-T025	Anti-Hu CD19 PE-Cy1537