Anti-Hu CD11c APC-Cy1537

Anti-Hu CD11c APC-Cy1537

• Background: CD11c (p150, alphaX integrin subunit) forms complex with CD18 (beta2 integrin subunit) and is expressed mainly on tissue macrophages and dendritic cells. CD11c binds to complement fragment iC3b, fibrinogen, VCAM-1 and ICAM-2 or e.g. CD90. Like other beta2 integrins, CD11c/CD18 plays roles in cell migration and phagocytosis. Moreover, interaction of CD11c/CD18 with plasminogen regulates plasmin activities, and interaction with heparin counteracts binding of iC3b.
• Specifications: The antibody BU15 reacts with an extracellular epitope of CD11c (alphaX, p150), a 150 kDa integrin expressed mainly on dendritic cells and tissue macrophages.
• CAS Number: 9007-83-4
• Certification: RUO
• Host: Mouse
• Species Reactivity: Human, Monkey
• Immunogen: Dendritic cells of synovial fluid
• Target Antigen: CD11c
• Isotype: IgG1
• Clone: BU15
• Applications: Flow cytometry: The reagent is designed for analysis of human blood cells using 4 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (0.4 mL) is sufficient for 100 tests.
• Validated Applications: FC
• Format: APC-Cy1537
• Buffer: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• References & Citations: *Van der Vieren M, Le Trong H, Wood CL, Moore PF, St John T, Staunton DE, Gallatin WM: A novel leukointegrin, alpha d beta 2, binds preferentially to ICAM-3. Immunity. 1995 Dec3(6):683-90., URL: http://www.ncbi.nlm.nih.gov/pubmed/8777714?ordinal,*Myou S, Zhu X, Boetticher E, Qin Y, Myo S, Meliton A, Lambertino A, Munoz NM, Hamann KJ, Leff AR: Regulation of adhesion of AML14.3D10 cells by surface clustering of beta2-integrin caused by ERK-independent activation of cPLA2. Immunology. 2002 Sep107(1):77-85., URL: http://www.ncbi.nlm.nih.gov/pubmed/12225365?ordina,*Sadhu C, Hendrickson L, Dick KO, Potter TG, Staunton DE: Novel Tools for Functional Analysis of CD11c: Activation-Specific, Activation-Independent, and Activating Antibodies. J Immunoassay Immunochem. 200829(1):42-57., URL: http://www.ncbi.nlm.nih.gov/pubmed/18080879?ordina,*Angel CE, Lala A, Chen CJ, Edgar SG, Ostrovsky LL, Dunbar PR: CD14+ antigen-presenting cells in human dermis are less mature than their CD1a+ counterparts. Int Immunol. 2007 Nov19(11):1271-9., URL: http://www.ncbi.nlm.nih.gov/pubmed/17804688?itool=,*Rezzonico R, Imbert V, Chicheportiche R, Dayer JM: Ligation of CD11b and CD11c beta(2) integrins by antibodies or soluble CD23 induces macrophage inflammatory protein 1alpha (MIP-1alpha) and MIP-1beta production in primary human monocytes through a pathway dependent on nuclear factor-kappaB. Blood. 2001 May 1597(10):2932-40., URL: http://www.ncbi.nlm.nih.gov/pubmed/11342414?ordina,*Leukocyte Typing III., McMichael A. J. et al (Eds.), Oxford University Press (1987)., URL: ,*Leukocyte Typing V., Schlossman S. et al. (Eds.), Oxford University Press (1995)., URL: ,*Leukocyte Typing VI., Kishimoto T. et al. (Eds.), Garland Publishing Inc. (1997)., URL: ,*Hogg N, Takacs L, Palmer DG, Selvendran Y, Allen C: The p150,95 molecule is a marker of human mononuclear phagocytes: comparison with expression of class II molecules. Eur J Immunol. 1986 Mar16(3):240-8., URL: http://www.ncbi.nlm.nih.gov/pubmed/3456894?ordinal,*Trowald-Wigh G, Johannisson A, H229kansson L: Canine neutrophil adhesion proteins and Fc-receptors in healthy dogs and dogs with adhesion protein deficiency, as studied by flow cytometry. Vet Immunol Immunopathol. 1993 Oct38(3-4):297-310., URL: http://www.ncbi.nlm.nih.gov/pubmed/8291207?ordinal,
• Other References: *Vorup-Jensen T, Chi L, Gjelstrup LC, Jensen UB, Jewett CA, Xie C, Shimaoka M, Linhardt RJ, Springer TA: Binding between the integrin alphaXbeta2 (CD11c/CD18) and heparin. J Biol Chem. 2007 Oct 19282(42):30869-77., URL: http://www.ncbi.nlm.nih.gov/pubmed/17699512?ordina,*Gang J, Choi J, Lee JH, Nham SU: Identification of critical residues for plasminogen binding by the alphaXI-domain of the beta2 integrin, alphaXbeta2. Mol Cells. 2007 Oct 3124(2):240-6., URL: http://www.ncbi.nlm.nih.gov/pubmed/17978577?ordina,*Sadhu C, Ting HJ, Lipsky B, Hensley K, Garcia-Martinez LF, Simon SI, Staunton DE: CD11c/CD18: novel ligands and a role in delayed-type hypersensitivity. J Leukoc Biol. 2007 Jun81(6):1395-403., URL: http://www.ncbi.nlm.nih.gov/pubmed/17389580?ordina,*Bullard DC, Hu X, Adams JE, Schoeb TR, Barnum SR: p150/95 (CD11c/CD18) expression is required for the development of experimental autoimmune encephalomyelitis. Am J Pathol. 2007 Jun170(6):2001-8., URL: http://www.ncbi.nlm.nih.gov/pubmed/17525267?ordina,
• Storage Conditions: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.