Anti-Hu CD3 APC-Cy™7

• Description:

  CD3 complex is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82% of normal peripheral blood lymphocytes, 65-85% of thymocytes and Purkynje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases.

• Certification:

  RUO

• Immunogen:

  Human thymocytes followed by Sezary T cells

• Target Antigen:

  CD3

• Clone:

  UCHT1

• Applications:

  FC (QC tested)

• Format:

  APC-Cy™7

• Buffer:

  Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

• References & Citations:

  *Rieux-Laucat F, Hivroz C, Lim A, Mateo V, Pellier I, Selz F, Fischer A, Le Deist F: Inherited and somatic CD3zeta mutations in a patient with T-cell deficiency. N Engl J Med. 2006 May 4;354 (18) :1913-21., URL: http://www.ncbi.nlm.nih.gov/pubmed/16672702?itool=, *Arnett KL, Harrison SC, Wiley DC: Crystal structure of a human CD3-epsilon/delta dimer in complex with a UCHT1 single-chain antibody fragment. Proc Natl Acad Sci U S A. 2004 Nov 16;101 (46) :16268-73., URL: https://www.ncbi.nlm.nih.gov/pubmed/15534202?itool, *Torres PS, Alcover A, Zapata DA, Arnaud J, Pacheco A, Martín-Fernández JM, Villasevil EM, Sanal O, Regueiro JR: TCR dynamics in human mature T lymphocytes lacking CD3 gamma. J Immunol. 2003 Jun 15;170 (12) :5947-55., URL: https://www.ncbi.nlm.nih.gov/pubmed/12794121?itool, *le Gouvello S, Manceau V, Sobel A: Serine 16 of stathmin as a cytosolic target for Ca2+/calmodulin-dependent kinase II after CD2 triggering of human T lymphocytes. J Immunol. 1998 Aug 1;161 (3) :1113-22. , URL: https://www.ncbi.nlm.nih.gov/pubmed/9686569?itool=, *Lin CW, Liu TY, Chen SU, Wang KT, Medeiros LJ, Hsu SM: CD94 1A transcripts characterize lymphoblastic lymphoma/leukemia of immature natural killer cell origin with distinct clinical features. Blood. 2005 Nov 15;106 (10) :3567-74., URL: https://www.ncbi.nlm.nih.gov/pubmed/16046525?itool, *Demedts IK, Brusselle GG, Vermaelen KY, Pauwels RA: Identification and characterization of human pulmonary dendritic cells. Am J Respir Cell Mol Biol. 2005 Mar;32 (3) :177-84., URL: https://www.ncbi.nlm.nih.gov/pubmed/15576669?itool, *Fisch P, Malkovsky M, Braakman E, Sturm E, Bolhuis RL, Prieve A, Sosman JA, Lam VA, Sondel PM: Gamma/delta T cell clones and natural killer cell clones mediate distinct patterns of non-major histocompatibility complex-restricted cytolysis. J Exp Med. 1990 May 1;171 (5) :1567-79., URL: https://www.ncbi.nlm.nih.gov/pubmed/2185329?ordina, *Siegers GM, Swamy M, Fernández-Malavé E, Minguet S, Rathmann S, Guardo AC, Pérez-Flores V, Regueiro JR, Alarcón B, Fisch P, Schamel WW: Different composition of the human and the mouse gammadelta T cell receptor explains different phenotypes of CD3gamma and CD3delta immunodeficiencies. J Exp Med. 2007 Oct 29;204 (11) :2537-44., URL: https://www.ncbi.nlm.nih.gov/pubmed/17923503?ordin, *Barclay, Brown et al.: The Leukocyte Antigen FactsBook, 2nd edition, Harcourt Brace & Company, London, (1997) ., URL: , *Leukocyte Typing VI., Kishimoto T. et al. (Eds.), Garland Publishing Inc. (1997) ., URL: , *Leukocyte Typing III., McMichael A. J. et al (Eds.), Oxford University Press (1987) ., URL: , *Garson JA, Beverley PC, Coakham HB, Harper EI: Monoclonal antibodies against human T lymphocytes label Purkinje neurones of many species. Nature. 1982 Jul 22;298 (5872) :375-7., URL: https://pubmed.ncbi.nlm.nih.gov/6178042/, *Dixon JF, Law JL, Favero JJ: Activation of human T lymphocytes by crosslinking of anti-CD3 monoclonal antibodies. J Leukoc Biol. 1989 Sep;46 (3) :214-20., URL: https://pubmed.ncbi.nlm.nih.gov/2788205/, *Verwilghen J, Baroja ML, Van Vaeck F, Van Damme J, Ceuppens JL: Differences in the stimulating capacity of immobilized anti-CD3 monoclonal antibodies: Variable dependence on interleukin-1 as a helper signal for T-cell activation. Immunology 1991 Feb;72 (2) :269-76., URL: https://pubmed.ncbi.nlm.nih.gov/1826671/, *Harper KS, Strefford JC, Cragg MS, Glennie MJ, Williams AP, French RR: Upregulation of FcγRIIb on monocytes is necessary to promote the superagonist activity of TGN1412. Blood 2015 Jan 1;125 (1) :102-10., URL: https://pubmed.ncbi.nlm.nih.gov/25395427/, *Quardokus EM, Saunders DC, McDonough E, Hickey JW, Werlein C, Surrette C, Rajbhandari P, Martinez Casals A, Tian H, Lowery L, Neumann EK, Björklund F, Neelakantan TV, Croteau J, Wiblin AE, Fisher J, Livengood AJ, Dowell KG, Silverstein JC, Spraggins JM, Pryhuber GS, Deutsch G, Ginty F, Nolan GP, Melov S, Jonigk D, Caldwell MA, Vlachos IS, Mueller W, Gehlenborg N, Stockwell BR, Lundberg E, Snyder MP, Germain RN, Camarillo JM, Kelleher NL, Börner K, Radtke AJ: Organ mapping antibody panels: a community resource for standardized multiplexed tissue imaging. Nat Methods 2023 Aug;20 (8) :1174-1178. doi: 10.1038/s41592-023-01846-7., URL: https://pubmed.ncbi.nlm.nih.gov/37468619/

• Other References:

  *Huang Y, Wange RL: T cell receptor signaling: beyond complex complexes. J Biol Chem. 2004 Jul 9;279 (28) :28827-30., URL: https://pubmed.ncbi.nlm.nih.gov/15084594/, *Alarcón B, Swamy M, van Santen HM, Schamel WW: T-cell antigen-receptor stoichiometry: pre-clustering for sensitivity. EMBO Rep. 2006 May;7 (5) :490-5., URL: https://pubmed.ncbi.nlm.nih.gov/16670682/, *Kuhns MS, Davis MM, Garcia KC: Deconstructing the form and function of the TCR/CD3 complex. Immunity. 2006 Feb;24 (2) :133-9., URL: https://pubmed.ncbi.nlm.nih.gov/16473826/

• Storage Conditions:

  Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

• Specificity:

  The antibody UCHT1 recognizes an extracellular epitope on CD3 antigen of the TCR/CD3 complex on mature human T cells. The UCHT1 antibody reacts with the epsilon chain of the CD3 complex.

• Applications Notes:

  6

• Species:

  Non-human primates, Human

• CAS Number:

  9007-83-4

• Isotype:

  Mouse IgG1 kappa