Anti-Hu CD38 APC-Cy1537

Anti-Hu CD38 APC-Cy1537

• Background: CD38 (NAD+ glycohydrolase) is a type II transmembrane glycoprotein able to induce activation, proliferation and differentiation of mature lymphocytes and mediate apoptosis of myeloid and lymphoid progenitor cells. Another role of CD38 is provided by enzymatic activity of its extracellular part. CD38 acts as NAD+ glycohydrolase converting NAD+ into ADP-ribose, as ADP-ribosyl cyclase producing cADPR and as cADPR hydrolase, thus affecting levels of calcium-mobilizing metabolites. ADPR produced by CD38 serves as an important second messenger of neutrophil and dendritic cell migration. CD38 belongs to markers of B cell subsets, leukemia, and a target of immunotherapy in myeloma treatment.
• Specifications: The mouse monoclonal antibody HIT2 reacts with an extracellular epitope of CD38, a 45 kDa type II transmembrane glycoprotein strongly expressed mainly on plasma cells and activated T and B lymphocytes it is an antigenic marker of lymphoid cells. Its binding is blocked by daratumumab.
• Certification: RUO
• Host: Mouse
• Species Reactivity: Human
• Immunogen: Human thymocytes in foetus
• Target Antigen: CD38
• Isotype: IgG1
• Clone: HIT2
• Applications: Flow cytometry: The reagent is designed for analysis of human blood cells using 4 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (0.4 mL) is sufficient for 100 tests.
• Validated Applications: FC
• Format: APC-Cy1537
• Buffer: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• References & Citations: *Leukocyte Typing III., McMichael AJ et al (Eds.), Oxford University Press (1987)., URL: ,*Rozková D, Novotná L, Pytlík R, Hochová I, Kozák T, Bartůnková J, Spísek R: Toll-like receptors on B-CLL cells: expression and functional consequences of their stimulation. Int J Cancer. 2010 Mar 1126(5):1132-43., URL: https://www.ncbi.nlm.nih.gov/pubmed/19685493,*Kolar GR, Mehta D, Pelayo R, Capra JD: A novel human B cell subpopulation representing the initial germinal center population to express AID. Blood. 2007 Mar 15109(6):2545-52., URL: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1852199/,*Všianská P, Říhová L, Varmužová T, Suská R, Kryukov F, Mikulášová A, Kupská R, Penka M, Pour L, Adam Z, Hájek R: Analysis of B-cell subpopulations in monoclonal gammopathies. Clin Lymphoma Myeloma Leuk. 2015 Apr15(4):e61-71., URL: http://www.ncbi.nlm.nih.gov/pubmed/25578543 ,*Ratuszny D, Skripuletz T, Wegner F, Groß M, Falk C, Jacobs R, Ruschulte H, Stangel M, Sühs KW: Case report: Daratumumab in a patient with severe refractory anti-NMDA receptor encephalitis. Front Neurol. 2020 Dec 2211:602102., URL: https://pubmed.ncbi.nlm.nih.gov/33414761/,
• Other References: *Cakir-Kiefer C, Muller-Steffner H, Oppenheimer N, Schuber F: Kinetic competence of the cADP-ribose-CD38 complex as an intermediate in the CD38/NAD+ glycohydrolase-catalysed reactions: implication for CD38 signalling. Biochem J. 2001 Sep 1358(Pt 2):399-406., URL: https://www.ncbi.nlm.nih.gov/pubmed/11513738?ordin,*Lund FE, Muller-Steffner H, Romero-Ramirez H, Moreno-García ME, Partida-Sánchez S, Makris M, Oppenheimer NJ, Santos-Argumedo L, Schuber F: CD38 induces apoptosis of a murine pro-B leukemic cell line by a tyrosine kinase-dependent but ADP-ribosyl cyclase- and NAD glycohydrolase-independent mechanism. Int Immunol. 2006 Jul18(7):1029-42., URL: https://www.ncbi.nlm.nih.gov/pubmed/16720618?ordin,*Partida-Sanchez S, Gasser A, Fliegert R, Siebrands CC, Dammermann W, Shi G, Mousseau BJ, Sumoza-Toledo A, Bhagat H, Walseth TF, Guse AH, Lund FE. Chemotaxis of mouse bone marrow neutrophils and dendritic cells is controlled by adp-ribose, the major product generated by the CD38 enzyme reaction. J Immunol. 2007 Dec 1179(11):7827-39., URL: https://www.ncbi.nlm.nih.gov/pubmed/18025229?ordin,
• Storage Conditions: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.