Anti-Hu CD4 Pacific Orange153

Anti-Hu CD4 Pacific Orange153

• Background: CD4 (T4) is a single chain transmembrane glycoprotein and belongs to immunoglobulin supergene family. In extracellular region there are 4 immunoglobulin-like domains (1 Ig-like V-type and 3 Ig-like C2-type). Transmembrane region forms 25 aa, cytoplasmic tail consists of 38 aa. Domains 1,2 and 4 are stabilized by disulfide bonds. The intracellular domain of CD4 is associated with p56Lck, a Src-like protein tyrosine kinase. It was described that CD4 segregates into specific detergent-resistant T-cell membrane microdomains. Extracellular ligands: MHC class II molecules (binds to CDR2-like region in CD4 domain 1) HIV envelope protein gp120 (binds to CDR2-like region in CD4 domain 1) IL-16 (binds to CD4 domain 3), human seminal plasma glycoprotein gp17 (binds to CD4 domain 1), L-selectin. Intracellular ligands: p56LckCD4 is a co-receptor involved in immune response (co-receptor activity in binding to MHC class II molecules) and HIV infection (human immunodeficiency virus CD4 is primary receptor for HIV-1 surface glycoprotein gp120). CD4 regulates T-cell activation, T/B-cell adhesion, T-cell diferentiation, T-cell selection and signal transduction. Defects in antigen presentation (MHC class II) cause dysfunction of CD4+ T-cells and their almost complete absence in patients blood, tissue and organs (SCID immunodeficiency).
• Specifications: The antibody MEM-241 recognizes an extracellular epitope of CD4 antigen, a 55 kDa transmebrane glycoprotein expressed on a subset of T lymphocytes ( 8220helper 8220 T-cells) and also on monocytes, tissue macrophages and granulocytes.
• CAS Number: 9007-83-4
• Certification: RUO
• Host: Mouse
• Species Reactivity: Human
• Immunogen: 2 N-terminal domains of human CD4 fused to human IgG1 Fc
• Target Antigen: CD4
• Isotype: IgG1
• Clone: MEM-241
• Applications: Flow cytometry: The reagent is designed for analysis of human blood cells using 4 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (0.4 mL) is sufficient for 100 tests.
• Validated Applications: FC
• Format: Pacific Orange153
• Buffer: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• References & Citations: *Brdickova N. et al.: LIME: a new membrane Raft-associated adaptor protein involved in CD4 and CD8 coreceptor signaling. J Exp Med. 2003 Nov 17198(10):1453-62. , URL: https://www.ncbi.nlm.nih.gov/pubmed/14610046?dopt=,*Karlsson KR, Cowley S, Martinez FO, Shaw M, Minger SL, James W: Homogeneous monocytes and macrophages from human embryonic stem cells following coculture-free differentiation in M-CSF and IL-3. Exp Hematol. 2008 Sep36(9):1167-75., URL: https://www.ncbi.nlm.nih.gov/pubmed/18550257?itool,*Manasa J, Musabaike H, Masimirembwa C, Burke E, Luthy R, Mudzori J: Evaluation of the Partec flow cytometer against the BD FACSCalibur system for monitoring immune responses of human immunodeficiency virus-infected patients in Zimbabwe. Clin Vaccine Immunol. 2007 Mar14(3):293-8., URL: https://www.ncbi.nlm.nih.gov/pubmed/17267593?itool,*Anderson AE, Sayers BL, Haniffa MA, Swan DJ, Diboll J, Wang XN, Isaacs JD, Hilkens CM: Differential regulation of naïve and memory CD4+ T cells by alternatively activated dendritic cells. J Leukoc Biol. 2008 Jul84(1):124-33., URL: https://www.ncbi.nlm.nih.gov/pubmed/18430785?itool,*Hovden AO, Karlsen M, Jonsson R, Aarstad HJ, Appel S: Maturation of monocyte derived dendritic cells with OK432 boosts IL-12p70 secretion and conveys strong T-cell responses. BMC Immunol. 2011 Jan 512:2., URL: https://www.ncbi.nlm.nih.gov/pubmed/21208424,*Zola H, Swart B, Banham A, Barry S, Beare A, Bensussan A, Boumsell L, D Buckley C, Buhring HJ, Clark G, Engel P, Fox D, Jin BQ, Macardle PJ, Malavasi F, Mason D, Stockinger H, Yang X: CD molecules 2006--human cell differentiation molecules. J Immunol Methods. 2007 Jan 30319(1-2):1-5. , URL: https://www.ncbi.nlm.nih.gov/pubmed/17174972?dopt=,*Kanderova V, Kuzilkova D, Stuchly J, Vaskova M, Brdicka T, Fiser K, Hrusak O, Lund-Johansen F, Kalina T: High-resolution Antibody Array Analysis of Childhood Acute Leukemia Cells. Mol Cell Proteomics. 2016 Apr15(4):1246-61., URL: http://www.ncbi.nlm.nih.gov/pubmed/26785729 ,*Ohradanova-Repic A, Machacek C, Charvet C, Lager F, Le Roux D, Platzer R, Leksa V, Mitulovic G, Burkard TR, Zlabinger GJ, Fischer MB, Feuillet V, Renault G, Blüml S, Benko M, Suchanek M, Huppa JB, Matsuyama T, Cavaco-Paulo A, Bismuth G, Stockinger H: Extracellular Purine Metabolism Is the Switchboard of Immunosuppressive Macrophages and a Novel Target to Treat Diseases With Macrophage Imbalances. Front Immunol. 2018 Apr 279:852., URL: https://www.ncbi.nlm.nih.gov/pubmed/29780382 ,*Černý V, Novotná O, Petrásková P Hudcová K, Boráková K, Prokešová L, Kolářová L, Hrdý J: Lower functional and proportional characteristics of cord blood treg of male newborns compared with female newborns. Biomedicines 2021, 9, 170., URL: https://pubmed.ncbi.nlm.nih.gov/33572097/,
• Other References: *Millan J, Cerny J, Horejsi V, Alonso MA: CD4 segregates into specific detergent-resistant T-cell membrane microdomains. Tissue Antigens. 1999 Jan53(1):33-40., URL: https://pubmed.ncbi.nlm.nih.gov/10082429/,
• Storage Conditions: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.