Anti-Hu CD41 Pacific Blue153

Anti-Hu CD41 Pacific Blue153

• Background: CD41 (platelet glycoprotein IIb) is composed of two subunits (120 kDa a, alpha and 23 kDa b, beta) that interact with CD61 in the presence of calcium to form a functional adhesive protein receptor. Upon blood vessel damage, this receptor binds to a variety of proteins including von Willebrand factor, fibrinogen, fibronectin and vitronectin. CD41 is mainly expressed on megakaryocyte-platelet lineage, but generally belongs to the antigens that are expressed during early stages of hematopoietic differentiation.
• Specifications: The antibody MEM-06 reacts with an extracellular epitope of CD41 (GPIIb), a transmembrane glycoprotein (integrin family) composed of two chains GPIIb alpha (heavy chain 120 kDa) and GPIIb beta (light chain 23 kDa). CD41 is mainly expressed on platelets and megakaryocytes.
• Certification: RUO
• Host: Mouse
• Species Reactivity: Human
• Immunogen: Leukocytes of patient suffering from LGL-type leukaemia.
• Target Antigen: CD41
• Isotype: IgG1
• Clone: MEM-06
• Applications: Flow cytometry: The reagent is designed for analysis of human blood cells using 4 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (0.4 mL) is sufficient for 100 tests.
• Validated Applications: FC
• Format: Pacific Blue153
• Buffer: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• References & Citations: *Belizaire RM, Prakash PS, Richter JR, Robinson BR, Edwards MJ, Caldwell CC, Lentsch AB, Pritts TA: Microparticles from stored red blood cells activate neutrophils and cause lung injury after hemorrhage and resuscitation. J Am Coll Surg. 2012 Apr214(4):648-55, URL: https://pubmed.ncbi.nlm.nih.gov/22342784/,*Pagani G, Pereira JPV, Stoldt VR, Beck A, Scharf RE, Gohlke H: The human platelet antigen-1b (Pro33) variant of 945IIb9463 allosterically shifts the dynamic conformational equilibrium of this integrin toward the active state. J Biol Chem. 2018 Mar 30293(13):4830-4844., URL: https://pubmed.ncbi.nlm.nih.gov/29462793/,
• Other References: *Mitjavila-Garcia MT, Cailleret M, Godin I, Nogueira MM, Cohen-Solal K, Schiavon V, Lecluse Y, Le Pesteur F, Lagrue AH, Vainchenker W: Expression of CD41 on hematopoietic progenitors derived from embryonic hematopoietic cells. Development. 2002 Apr129(8):2003-13., URL: https://www.ncbi.nlm.nih.gov/pubmed/11934866?ordin,*Ferkowicz MJ, Starr M, Xie X, Li W, Johnson SA, Shelley WC, Morrison PR, Yoder MC: CD41 expression defines the onset of primitive and definitive hematopoiesis in the murine embryo. Development. 2003 Sep130(18):4393-403., URL: https://www.ncbi.nlm.nih.gov/pubmed/12900455?ordin,*Zhang J, Varas F, Stadtfeld M, Heck S, Faust N, Graf T: CD41-YFP mice allow in vivo labeling of megakaryocytic cells and reveal a subset of platelets hyperreactive to thrombin stimulation. Exp Hematol. 2007 Mar35(3):490-499., URL: https://www.ncbi.nlm.nih.gov/pubmed/17309829?ordin,*Debili N, Robin C, Schiavon V, Letestu R, Pflumio F, Mitjavila-Garcia MT, Coulombel L, Vainchenker W: Different expression of CD41 on human lymphoid and myeloid progenitors from adults and neonates. Blood. 2001 Apr 197(7):2023-30., URL: https://www.ncbi.nlm.nih.gov/pubmed/11264167?ordin,
• Storage Conditions: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.