Anti-Hu CD48 Pacific Blue153

Anti-Hu CD48 Pacific Blue153

• Background: CD48 (Blast-1) belongs to the CD2 subset of the Ig superfamily, which includes CD2, CD2F-10, CD58, CD84, CD150, CD229, CD244 and others. These molecules bind to the same or another members of their family, thus mediate homotypic or heterotypic adhesion. CD48 is a GPI-anchored protein broadly expressed on hematopoietic cells and serves as a high affinity ligand for 2B4 and low affinity ligand for CD2. 2B4-CD48 interaction among NK cells and NK-T cells regulates cell proliferation. Signaling through CD48 results in eosinophil activation and CD48 expression is increased in several infectious diseases.
• Specifications: The antibody MEM-102 reacts with CD48 (Blast-1), a 40-47 kDa GPI-anchored extracellular membrane protein (immunoglobulin supergene family) widely expressed on hematopoietic cells it is negative on granulocytes, platelets and erythrocytes.
• CAS Number: 9007-83-4
• Certification: RUO
• Host: Mouse
• Species Reactivity: Non-human primates, Human
• Immunogen: Raji human Burkitt's lymphoma cell line
• Target Antigen: CD48
• Isotype: IgG1
• Clone: MEM-102
• Applications: Flow cytometry: The reagent is designed for analysis of human blood cells using 4 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (0.4 mL) is sufficient for 100 tests.
• Validated Applications: FC
• Format: Pacific Blue153
• Buffer: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• References & Citations: *Leukocyte Typing IV., Knapp W. et al. (Eds.), Oxford University Press (1989)., URL: ,*Korinek V, Stefanova I, Angelisova P, Hilgert I, Horejsi V: The human leucocyte antigen CD48 (MEM-102) is closely related to the activation marker Blast-1. Immunogenetics. 199133(2):108-12., URL: https://www.ncbi.nlm.nih.gov/pubmed/1999350?dopt=A,*Leukocyte Typing V., Schlossman S. et al. (Eds.), Oxford University Press (1995)., URL: ,*Drbal K, Moertelmaier M, Holzhauser C, Muhammad A, Fuertbauer E, Howorka S, Hinterberger M, Stockinger H, Schütz GJ: Single-molecule microscopy reveals heterogeneous dynamics of lipid raft components upon TCR engagement. Int Immunol. 2007 May19(5):675-84., URL: https://www.ncbi.nlm.nih.gov/pubmed/17446208?itool,*Angelisová P, Drbal K, Horejsí V, Cerný J: Association of CD10/neutral endopeptidase 24.11 with membrane microdomains rich in glycosylphosphatidylinositol-anchored proteins and Lyn kinase. Blood. 1999 Feb 1593(4):1437-9., URL: https://www.ncbi.nlm.nih.gov/pubmed/10075459?itool,*Stulnig TM, Berger M, Sigmund T, Stockinger H, Horejsí V, Waldhäusl W: Signal transduction via glycosyl phosphatidylinositol-anchored proteins in T cells is inhibited by lowering cellular cholesterol. J Biol Chem. 1997 Aug 1272(31):19242-7., URL: https://www.ncbi.nlm.nih.gov/pubmed/9235917,*Bazil V, Stefanova I, Hilgert I, Kristofova H, Vanek S, Bukovsky A, Horejsi V: Monoclonal antibodies against human leucocyte antigens. III. Antibodies against CD45R, CD6, CD44 and two newly described broadly expressed glycoproteins MEM-53 and MEM-102. Folia Biol (Praha). 198935(5):289-97. , URL: https://www.ncbi.nlm.nih.gov/pubmed/2534480?dopt=A,*Schatzlmaier P, Supper V, Göschl L, Zwirzitz A, Eckerstorfer P, Ellmeier W, Huppa JB, Stockinger H: Rapid multiplex analysis of lipid raft components with single-cell resolution. Sci Signal. 2015 Sep 228(395):rs11, URL: http://www.ncbi.nlm.nih.gov/pubmed/26396269 ,
• Other References: *Assarsson E, Kambayashi T, Persson CM, Chambers BJ, Ljunggren HG: 2B4/CD48-mediated regulation of lymphocyte activation and function. J Immunol. 2005 Aug 15175(4):2045-9., URL: https://www.ncbi.nlm.nih.gov/pubmed/16081768?ordin,*Mathew SO, Kumaresan PR, Lee JK, Huynh VT, Mathew PA: Mutational analysis of the human 2B4 (CD244)/CD48 interaction: Lys68 and Glu70 in the V domain of 2B4 are critical for CD48 binding and functional activation of NK cells. J Immunol. 2005 Jul 15175(2):1005-13., URL: https://www.ncbi.nlm.nih.gov/pubmed/16002700?ordin,*Lee KM, Forman JP, McNerney ME, Stepp S, Kuppireddi S, Guzior D, Latchman YE, Sayegh MH, Yagita H, Park CK, Oh SB, Wülfing C, Schatzle J, Mathew PA, Sharpe AH, Kumar V: Requirement of homotypic NK-cell interactions through 2B4(CD244)/CD48 in the generation of NK effector functions. Blood. 2006 Apr 15107(8):3181-8., URL: https://www.ncbi.nlm.nih.gov/pubmed/15905190?ordin,*Munitz A, Bachelet I, Eliashar R, Khodoun M, Finkelman FD, Rothenberg ME, Levi-Schaffer F: CD48 is an allergen and IL-3-induced activation molecule on eosinophils. J Immunol. 2006 Jul 1177(1):77-83., URL: https://www.ncbi.nlm.nih.gov/pubmed/16785501?ordin,
• Storage Conditions: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.