Mouse IgG2a Isotype Control DyLight® 488

Mouse IgG2a Isotype Control DyLight® 488

• Specifications: This mouse IgG2a monoclonal antibody (clone MOPC-173) reacts with an unknown epitope. It does not react with a variety of resting, activated, live, and fixed mouse, rat and human tissues.
• Certification: RUO
• Host: Mouse
• Negative Species: Mouse, Rat, Human
• Immunogen: The transplantable plasmacytoma MOPC-173 was induced by intraperitoneal injection of mineral oils into BALB/c mice.
• Isotype: IgG2a κ
• Clone: MOPC-173
• Applications: Negative control: The reagent is intended as an isotype control to establish the amount of non-specific antibody binding. For your particular experiment, use the same concentration of this control antibody as the recommended working concentration of the antigen-specific antibody. Also, when working with prediluted antibodies, dilute the isotype control to the same concentration as is the concentration of the antigen-specific antibody in the prediluted antibody solution you are using. If under particular experimental conditions the background signal of the isotype control is too high (usually when working concentrations of used antibodies are above 10 μg/mL of incubation mixture), change the conditions of your experiment to reduce the background.
• Validated Applications: FC, IHC-P, IHC-F, ICC, WB
• Concentration: 0.1 mg/mL
• Format: DyLight® 488
• Buffer: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• References & Citations: *Fougereau M, Bourgois A, de Preval C, Rocca-Serra J, Schiff C: The complete sequence of the murine monoclonal immunoglobulin MOPC 173 (IgG2a): genetic implications. Ann Immunol (Paris). 1976 Sep-Oct127(5):607-31., URL: http://www.ncbi.nlm.nih.gov/pubmed/984731,*Baumal R, Scharff MD: Immunoglobulin biosynthesis by the MOPC 173 mouse myeloma tumor and a variant spleen clone. J Immunol. 1976 Jan116(1):65-74., URL: http://www.ncbi.nlm.nih.gov/pubmed/812916 ,*Gupta V, Gylling A, Alonso JL, Sugimori T, Ianakiev P, Xiong JP, Arnaout MA: The beta-tail domain (betaTD) regulates physiologic ligand binding to integrin CD11b/CD18. Blood. 2007 Apr 15109(8):3513-20., URL: http://www.ncbi.nlm.nih.gov/pubmed/?term=gupta+v+gylling+a ,*Khoddami V, Cairns BR: Transcriptome-wide target profiling of RNA cytosine methyltransferases using the mechanism-based enrichment procedure Aza-IP. Nat Protoc. 2014 Feb9(2):337-61., URL: http://www.ncbi.nlm.nih.gov/pubmed/24434802 ,
• Storage Conditions: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.