Anti-Hu CD161 Alexa Fluor® 700

Anti-Hu CD161 Alexa Fluor® 700

• Background: CD161, also known as Nkrp1 (natural killer receptor protein 1) or Klrb1 (killer cell lectin-like receptor subfamily b member 1), is a disulphide-linked homodimeric receptor, which is involved in regulation of NK cell and NKT cell function. It is expressed on rat NK cells, subset of T cells, dendritic cells, and activated monocytes. Although human CD161 is expressed as one isoform, the rat CD161 has three isoforms, referred to as CD161a, b, and c. These proteins contain C-terminal C-type lectin extracellular domain, a transmembrane domain, and N-terminal intracellular domain, which contains ITIM motif, such as CD161b, and displays inhibitory function, or does not contain ITIM motif, thus also not the inhibitory function, such as CD161a.
• Specifications: The mouse monoclonal antibody HP-3G10 recognizes an extracellular epitope of CD161, a type II transmembrane C-type lectin receptor, expressed on the plasma membrane of NK cells, dendritic cells, activated monocytes and a subset of T cells as a disulphide-linked homodimer.
• CAS Number: 9007-83-4
• Certification: RUO
• Host: Mouse
• Species Reactivity: Non-human primates, Human
• Immunogen: human NK cells
• Target Antigen: CD161
• Isotype: Igg1 κ
• Clone: HP-3G10
• Applications: Flow cytometry: The reagent is designed for analysis of human blood cells using 4 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (0.4 mL) is sufficient for 100 tests.
• Validated Applications: FC
• Format: Alexa Fluor® 700
• Buffer: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• References & Citations: *Goetzl EJ, Huang MC, Kon J, Patel K, Schwartz JB, Fast K, Ferrucci L, Madara K, Taub DD, Longo DL: Gender specificity of altered human immune cytokine profiles in aging. FASEB J. 2010 Sep24(9):3580-9., URL: http://www.ncbi.nlm.nih.gov/pubmed/?term=goetzl+ej+kon+j ,*Tresoldi E, Dell'Albani I, Stabilini A, Jofra T, Valle A, Gagliani N, Bondanza A, Roncarolo MG, Battaglia M: Stability of human rapamycin-expanded CD4+CD25+ T regulatory cells. Haematologica. 2011 Sep96(9):1357-65., URL: http://www.ncbi.nlm.nih.gov/pubmed/21565906 ,*Birgit Fogal, Tai Yi, Chen Wang, Deepak A. Rao, Amir Lebastchi, Sanjay Kulkarni, George Tellides, Jordan S. Pober: Neutralizing IL-6 reduces human arterial allograft rejection by allowing emergence of CD161(+) CD4(+) T regulatory cells. J Immunol. 2011 December 15 187(12): 626882116280. , URL: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC323782,*Wong EB, Akilimali NA, Govender P, Sullivan ZA, Cosgrove C, Pillay M, Lewinsohn DM, Bishai WR, Walker BD, Ndung'u T, Klenerman P, Kasprowicz VO: Low levels of peripheral CD161++CD8+ mucosal associated invariant T (MAIT) cells are found in HIV and HIV/TB co-infection. PLoS One. 2013 Dec 318(12):e83474, URL: https://pubmed.ncbi.nlm.nih.gov/24391773/,*Germain C, Meier A, Jensen T, Knapnougel P, Poupon G, Lazzari A, Neisig A, H229kansson K, Dong T, Wagtmann N, Galsgaard ED, Spee P, Braud VM: Induction of lectin-like transcript 1 (LLT1) protein cell surface expression by pathogens and interferon-947 contributes to modulate immune responses. J Biol Chem. 2011 Nov 4286(44):37964-75, URL: http://www.ncbi.nlm.nih.gov/pubmed/21930700 ,*Pozo D, Valés-Gómez M, Mavaddat N, Williamson SC, Chisholm SE, Reyburn H: CD161 (human NKR-P1A) signaling in NK cells involves the activation of acid sphingomyelinase. J Immunol. 2006 Feb 15176(4):2397-406., URL: http://www.ncbi.nlm.nih.gov/pubmed/?term=pozo+d+mavaddat+n,*Quardokus EM, Saunders DC, McDonough E, Hickey JW, Werlein C, Surrette C, Rajbhandari P, Martinez Casals A, Tian H, Lowery L, Neumann EK, Björklund F, Neelakantan TV, Croteau J, Wiblin AE, Fisher J, Livengood AJ, Dowell KG, Silverstein JC, Spraggins JM, Pryhuber GS, Deutsch G, Ginty F, Nolan GP, Melov S, Jonigk D, Caldwell MA, Vlachos IS, Mueller W, Gehlenborg N, Stockwell BR, Lundberg E, Snyder MP, Germain RN, Camarillo JM, Kelleher NL, Börner K, Radtke AJ: Organ mapping antibody panels: a community resource for standardized multiplexed tissue imaging. Nat Methods 2023 Aug20(8):1174-1178. doi: 10.1038/s41592-023-01846-7., URL: https://pubmed.ncbi.nlm.nih.gov/37468619/,
• Storage Conditions: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.