Anti-Hu CD279 Alexa Fluor® 700

Anti-Hu CD279 Alexa Fluor® 700

• Background: CD279 / PD-1 (programmed cell death 1), a transmembrane protein of CD28/CTLA-4 family. It is expressed inducibly mainly on activated T, B, and myeloid cells and plays a role in maintaining peripheral self-tolerance. Binding to its ligands CD273 and CD274 is associated with inhibition of T cell proliferation and induction of their anergy. It is also expressed during thymic development. Some variants of CD279 are associated with susceptibility to systemic lupus erythematosus, type 1 diabetes, and rheumatoid arthritis.
• Specifications: The mouse monoclonal antibody EH12.2H7 recognizes an extracellular epitope of CD279 / PD-1 (programmed cell death 1), a 55 kDa type I transmembrane protein expressed above all during T cell development, on activated T cells, activated B cells, and activated monocytes.
• Certification: RUO
• Host: Mouse
• Species Reactivity: Non-human primates, Human
• Immunogen: recombinant human CD279
• Target Antigen: CD279
• Isotype: Igg1 κ
• Clone: EH12.2H7
• Applications: Flow cytometry: The reagent is designed for analysis of human blood cells using 4 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (0.4 mL) is sufficient for 100 tests.
• Validated Applications: FC
• Format: Alexa Fluor® 700
• Buffer: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• References & Citations: *Xu Y, Weatherall C, Bailey M, Alcantara S, De Rose R, Estaquier J, Wilson K, Suzuki K, Corbeil J, Cooper DA, Kent SJ, Kelleher AD, Zaunders J: Simian immunodeficiency virus infects follicular helper CD4 T cells in lymphoid tissues during pathogenic infection of pigtail macaques. J Virol. 2013 Apr87(7):3760-73., URL: https://pubmed.ncbi.nlm.nih.gov/23325697/,*Bedoret D, Singh AK, Shaw V, Hoyte EG, Hamilton R, DeKruyff RH, Schneider LC, Nadeau KC, Umetsu DT: Changes in antigen-specific T-cell number and function during oral desensitization in cow's milk allergy enabled with omalizumab. Mucosal Immunol. 2012 May5(3):267-76., URL: https://pubmed.ncbi.nlm.nih.gov/22318492/,*Gros A, Robbins PF, Yao X, Li YF, Turcotte S, Tran E, Wunderlich JR, Mixon A, Farid S, Dudley ME, Hanada K, Almeida JR, Darko S, Douek DC, Yang JC, Rosenberg SA: PD-1 identifies the patient-specific CD88314 tumor-reactive repertoire infiltrating human tumors. J Clin Invest. 2014 May124(5):2246-59., URL: https://pubmed.ncbi.nlm.nih.gov/24667641/,*Haile ST, Dalal SP, Clements V, Tamada K, Ostrand-Rosenberg S: Soluble CD80 restores T cell activation and overcomes tumor cell programmed death ligand 1-mediated immune suppression. J Immunol. 2013 Sep 1191(5):2829-36., URL: http://www.ncbi.nlm.nih.gov/pubmed/?term=haile+st+dalal+sp+clements+v ,*Černý V, Hrdý J, Novotná O, Petrásková P, Boráková K, Kolářová L, Prokešová L: Distinct characteristics of Tregs of newborns of healthy and allergic mothers. PLoS One. 2018 Nov 2613(11):e0207998. , URL: https://www.ncbi.nlm.nih.gov/pubmed/30475891 ,*Zhou J, Cheung AK, Liu H, Tan Z, Tang X, Kang Y, Du Y, Wang H, Liu L, Chen Z: Potentiating functional antigen-specific CD88314 T cell immunity by a novel PD1 isoform-based fusion DNA vaccine. Mol Ther. 2013 Jul21(7):1445-55., URL: http://www.ncbi.nlm.nih.gov/pubmed/?term=zhou+j+tan+z+tang+x,*Li H, Pauza CD: CD25(+) Bcl6(low) T follicular helper cells provide help to maturing B cells in germinal centers of human tonsil. Eur J Immunol. 2015 Jan45(1):298-308. , URL: https://pubmed.ncbi.nlm.nih.gov/25263533/,*Quardokus EM, Saunders DC, McDonough E, Hickey JW, Werlein C, Surrette C, Rajbhandari P, Martinez Casals A, Tian H, Lowery L, Neumann EK, Björklund F, Neelakantan TV, Croteau J, Wiblin AE, Fisher J, Livengood AJ, Dowell KG, Silverstein JC, Spraggins JM, Pryhuber GS, Deutsch G, Ginty F, Nolan GP, Melov S, Jonigk D, Caldwell MA, Vlachos IS, Mueller W, Gehlenborg N, Stockwell BR, Lundberg E, Snyder MP, Germain RN, Camarillo JM, Kelleher NL, Börner K, Radtke AJ: Organ mapping antibody panels: a community resource for standardized multiplexed tissue imaging. Nat Methods 2023 Aug20(8):1174-1178. doi: 10.1038/s41592-023-01846-7., URL: https://pubmed.ncbi.nlm.nih.gov/37468619/,
• Storage Conditions: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.