Anti-Hu CD31 Alexa Fluor® 488

Anti-Hu CD31 Alexa Fluor® 488

• Background: CD31 (platelet endothelial cell adhesion molecule-1, PECAM-1) is an inhibitory coreceptor involved in regulation of T cell and B cell signaling by a dual immunoreceptor tyrosine-based inhibitory motif (ITIM) that upon associated kinases-mediated phosphorylation provide docking sites for protein-tyrosine phosphatases. CD31 is expressed ubiquitously within the vascular compartment and is located mainly at junctions between adjacent cells. N-terminal Ig-like domain of CD31 is responsible for its homophilic binding, which plays an important role in cell-cell interactions. CD31 is a multifunctional molecule with diverse roles in modulation of integrin-mediated cell adhesion, transendothelial migration, angiogenesis, apoptosis, negative regulation of immunoreceptor signaling, autoimmunity, macrophage phagocytosis, IgE-mediated anaphylaxis and thrombosis. It is one of key regulatory molecules in vascular system.
• Specifications: The antibody MEM-05 reacts with an extracellular epitope of CD31 (PECAM-1), a 130-140 kDa type I transmembrane glycoprotein expressed on monocytes, platelets, granulocytes, endothelial cells and stem cells of the myeloid lineage.
• CAS Number: 9007-83-4
• Certification: RUO
• Host: Mouse
• Species Reactivity: Human
• Immunogen: Leukocytes of a patient suffering from LGL-type leukaemia
• Target Antigen: CD31
• Isotype: IgG1
• Clone: MEM-05
• Applications: Flow cytometry: The reagent is designed for analysis of human blood cells using 4 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (0.4 mL) is sufficient for 100 tests.
• Validated Applications: FC, WB, IHC-P, ICC
• Format: Alexa Fluor® 488
• Buffer: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• References & Citations: *Prager E, Staffler G, Majdic O, Saemann M, Godar S, Zlabinger G, Stockinger H: Induction of hyporesponsiveness and impaired T lymphocyte activation by the CD31 receptor:ligand pathway in T cells. J Immunol. 2001 Feb 15166(4):2364-71. , URL: https://www.ncbi.nlm.nih.gov/pubmed/11160294?dopt=,*Cârţână T, Săftoiu A, Gruionu LG, Gheonea DI, Pirici D, Georgescu CV, Ciocâlteu A, Gruionu G: Confocal laser endomicroscopy for the morphometric evaluation of microvessels in human colorectal cancer using targeted anti-CD31 antibodies. PLoS One. 20127(12):e52815. doi: 10.1371/journal.pone.0052815., URL: https://pubmed.ncbi.nlm.nih.gov/23285192/,*Kalina T, Bakardjieva M, Blom M, Perez-Andres M, Barendregt B, Kanderová V, Bonroy C, Philippé J, Blanco E, Pico-Knijnenburg I, Paping JH, Wolska-Kuśnierz B, Pac M, Tkazcyk J, Haerynck F, Akar HH, Formánková R, Freiberger T, Svatoň M, Šedivá A, Arriba-Méndez S, Orfao A, van Dongen JJ, van der Burg M: EuroFlow standardized approach to diagnostic immunopheneotyping of severe PID innNewborns and young children. Front Immunol. 2020 Mar 1911:371., URL: https://pubmed.ncbi.nlm.nih.gov/32265901/,*Wei F, Cao S, Ren X, Liu H, Yu J, Li H, Hao X: Efficient antiproliferative and antiangiogenic effects on human ovarian cancer growth by gene transfer of attenuated mutants of Shiga-like toxin I. Int J Gynecol Cancer. Jul-Aug 200818(4):677-91., URL: https://pubmed.ncbi.nlm.nih.gov/17944920/,
• Other References: *Wong MX, Hayball JD, Jackson DE: PECAM-1-regulated signalling thresholds control tolerance in anergic transgenic B-cells. Mol Immunol. 2007 Oct 29, URL: https://www.ncbi.nlm.nih.gov/pubmed/17977600?ordin,*Woodfin A, Voisin MB, Nourshargh S: PECAM-1: a multi-functional molecule in inflammation and vascular biology. Arterioscler Thromb Vasc Biol. 2007 Dec27(12):2514-23., URL: https://www.ncbi.nlm.nih.gov/pubmed/17872453?ordin,*Wee JL, Jackson DE: The Ig-ITIM superfamily member PECAM-1 regulates the "outside-in" signaling properties of integrin alpha(IIb)beta3 in platelets. Blood. 2005 Dec 1106(12):3816-23., URL: https://www.ncbi.nlm.nih.gov/pubmed/16081692?ordin,*Jackson DE: The unfolding tale of PECAM-1. FEBS Lett. 2003 Apr 10540(1-3):7-14., URL: https://www.ncbi.nlm.nih.gov/pubmed/12681475?ordin,*Wilkinson R, Lyons AB, Roberts D, Wong MX, Bartley PA, Jackson DE: Platelet endothelial cell adhesion molecule-1 (PECAM-1/CD31) acts as a regulator of B-cell development, B-cell antigen receptor (BCR)-mediated activation, and autoimmune disease. Blood. 2002 Jul 1100(1):184-93., URL: https://www.ncbi.nlm.nih.gov/pubmed/12070026?ordin,*Newman DK, Hamilton C, Newman PJ: Inhibition of antigen-receptor signaling by Platelet Endothelial Cell Adhesion Molecule-1 (CD31) requires functional ITIMs, SHP-2, and p56(lck). Blood. 2001 Apr 1597(8):2351-7., URL: https://www.ncbi.nlm.nih.gov/pubmed/11290597?ordin,
• Storage Conditions: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.