Products for Research Use Only
RegisterLogin

Anti-Hu CD4 PE

CAT: 0270-1P-816-T100Size: 100 TestsDry Ice: NoHazardous: No
Product image 1
1 / 1
CAT#:0270-1P-816-T100Size:100 Tests
Selected
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
Quick Request Actions
Description
CD4 (T4) is a single chain transmembrane glycoprotein and belongs to immunoglobulin supergene family. In extracellular region there are 4 immunoglobulin-like domains (1 Ig-like V-type and 3 Ig-like C2-type) . Transmembrane region forms 25 aa, cytoplasmic tail consists of 38 aa. Domains 1,2 and 4 are stabilized by disulfide bonds. The intracellular domain of CD4 is associated with p56Lck, a Src-like protein tyrosine kinase. It was described that CD4 segregates into specific detergent-resistant T-cell membrane microdomains. Extracellular ligands: MHC class II molecules (binds to CDR2-like region in CD4 domain 1) ; HIV envelope protein gp120 (binds to CDR2-like region in CD4 domain 1) ; IL-16 (binds to CD4 domain 3), human seminal plasma glycoprotein gp17 (binds to CD4 domain 1), L-selectin. Intracellular ligands: p56LckCD4 is a co-receptor involved in immune response (co-receptor activity in binding to MHC class II molecules) and HIV infection (human immunodeficiency virus; CD4 is primary receptor for HIV-1 surface glycoprotein gp120) . CD4 regulates T-cell activation, T/B-cell adhesion, T-cell diferentiation, T-cell selection and signal transduction. Defects in antigen presentation (MHC class II) cause dysfunction of CD4+ T-cells and their almost complete absence in patients blood, tissue and organs (SCID immunodeficiency) .
Certification
RUO
Reactivity
Human
Immunogen
Normal human blood lymphocytes
Target Antigen
CD4
Clone
EM4
Applications
FC (QC tested)
Format
PE
Buffer
Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
References & Citations
*Filatov AV, Krotov GI, Zgoda VG, Volkov Y: Fluorescent immunoprecipitation analysis of cell surface proteins: a methodology compatible with mass-spectrometry. J Immunol Methods. 2007 Jan 30;319 (1-2) :21-33., URL: http://www.ncbi.nlm.nih.gov/pubmed/17098248
Other References
*Millan J, Cerny J, Horejsi V, Alonso MA: CD4 segregates into specific detergent-resistant T-cell membrane microdomains. Tissue Antigens. 1999 Jan;53 (1) :33-40., URL: https://pubmed.ncbi.nlm.nih.gov/10082429/
Storage Conditions
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Specificity
The mouse monoclonal antibody EM4 recognizes an extracellular epitope of CD4 antigen, a 55 kDa transmebrane glycoprotein expressed on a subset of T lymphocytes (“ helper“ T-cells) and also on monocytes, tissue macrophages and granulocytes. This antibody does not block Leu3a and OKT4 binding, and blocks HIV-1 infection in cell to cell system. Very strong flow cytometry staining, brighter than Leu3a, OKT4 and other.
Applications Notes
6
Isotype
Mouse IgG2a

Related Products

CatalogName
11-359-C100Anti-Hu CD4 Purified
1A-359-T025Anti-Hu CD4 APC
PC-359-T025Anti-Hu CD4 PerCP
11-203-C100Anti-Hu CD4 Purified
11-203-C025Anti-Hu CD4 Purified
1F-359-T100Anti-Hu CD4 FITC
1F-359-T025Anti-Hu CD4 FITC
1B-359-C100Anti-Hu CD4 Biotin
1B-359-C025Anti-Hu CD4 Biotin
11-816-C100Anti-Hu CD4 Purified

Alternative Products

CatalogName
18184002Anti-Hu IgG
MBS1801698-01Anti-Hu SUSD2
MBS1800499-01Anti-Hu CD102 PE
MBS1800115-01Anti-Hu CD45 APC
MBS1802517-01Anti-Hu C5aR2 APC
MBS1802513-01Anti-Hu CD105 APC
MBS1802509-01Anti-Hu CD141 PE
MBS1802497-01Anti-Hu CD187 APC
MBS1802495-01Anti-Hu CD24 Biotin
MBS1802494-01Anti-Hu CD266 APC

Popular Products