Anti-Hu CD34 PE

Availability: 24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Alternative Products

Cat	Name
MBS1801497-02	Anti-Hu CD34 PE
MBS1800214-01	Anti-Hu CD34 Biotin
MBS1800246-01	Anti-Hu CD34 PerCP
MBS1800246-02	Anti-Hu CD34 PerCP
MBS1800244-02	Anti-Hu CD34 FITC
MBS1801496-02	Anti-Hu CD34 FITC
MBS1800245-02	Anti-Hu CD34 PE
MBS1800215-02	Anti-Hu CD34 FITC
MBS1800244-01	Anti-Hu CD34 FITC
MBS1801496-01	Anti-Hu CD34 FITC

Anti-Hu CD34 PE

CAT:

270-1P-664-T100

Size:

100 Tests

Price:

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For price, please contact [email protected].

Anti-Hu CD34 PE

Background: CD34 is a highly glycosylated monomeric 111-115 kDa surface protein, which is present on many stem cell populations. It is a well established stem cell marker, though its expression on human hematopoietic stem cells is reversible. CD34 probably serves as a surface receptor that undergoes receptor-mediated endocytosis and regulates adhesion, differentiation and proliferation of hematopoietic stem cells and other progenitors. CD34 expression is likely to represent a specific state of hematopoietic development that may have altered adhering properties with expanding and differentiating capabilities in both in vitro and in vivo conditions.
Specifications: The mouse monoclonal antibody 581 reacts with an extracellular epitope of CD34, a 110-115 kDa monomeric transmembrane phosphoglycoprotein expressed on hematopoietic progenitors cells and on the most pluripotential stem cells it is gradually lost on progenitor cells. The antibody recognizes the class III CD34 epitope resistant to neuraminidase, chymopapain and glycoprotease.
Certification: RUO
Host: Mouse
Species Reactivity: Non-human primates, Human
Negative Species: Sheep
Target Antigen: CD34
Isotype: Igg1 κ
Clone: 581
Applications: Flow cytometry: The reagent is designed for analysis of human blood cells using 20 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (2 mL) is sufficient for 100 tests.
Validated Applications: FC
Format: PE
Buffer: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
References & Citations: *Sanz E, Mu241oz-A N, Monserrat J, Van-Den-Rym A, Escoll P, Ranz I, Alvarez-Mon M, de-la-Hera A: Ordering human CD34+CD10-CD19+ pre/pro-B-cell and CD19- common lymphoid progenitor stages in two pro-B-cell development pathways. Proc Natl Acad Sci U S A. 2010 Mar 30107(13):5925-30., URL: http://www.ncbi.nlm.nih.gov/pubmed/20231472 ,*Goardon N, Nikolousis E, Sternberg A, Chu WK, Craddock C, Richardson P, Benson R, Drayson M, Standen G, Vyas P, Freeman S: Reduced CD38 expression on CD34+ cells as a diagnostic test in myelodysplastic syndromes. Haematologica. 2009 Aug94(8):1160-3., URL: http://www.ncbi.nlm.nih.gov/pubmed/19644143 ,*Iwasaki H, Kawamoto A, Ishikawa M, Oyamada A, Nakamori S, Nishimura H, Sadamoto K, Horii M, Matsumoto T, Murasawa S, Shibata T, Suehiro S, Asahara T: Dose-dependent contribution of CD34-positive cell transplantation to concurrent vasculogenesis and cardiomyogenesis for functional regenerative recovery after myocardial infarction. Circulation. 2006 Mar 14113(10):1311-25., URL: https://www.ncbi.nlm.nih.gov/pubmed/16534028,*Ninos JM, Jefferies LC, Cogle CR, Kerr WG: The thrombopoietin receptor, c-Mpl, is a selective surface marker for human hematopoietic stem cells. J Transl Med. 2006 Feb 164:9., URL: https://pubmed.ncbi.nlm.nih.gov/16480521/,*Ono F, Sharma BK, Smith CC, Burnett JW, Aurelian L: CD34+ cells in the peripheral blood transport herpes simplex virus DNA fragments to the skin of patients with erythema multiforme (HAEM).J Invest Dermatol. 2005 Jun124(6):1215-24., URL: https://pubmed.ncbi.nlm.nih.gov/15955097/,*Suárez L, Vidriales MB, García-Laraña J, Sanz G, Moreno MJ, López A, Barrena S, Martínez R, Tormo M, Palomera L, Lavilla E, López-Berges MC, de Santiago M, de Equiza ME, Miguel JF, Orfao A: CD34+ cells from acute myeloid leukemia, myelodysplastic syndromes, and normal bone marrow display different apoptosis and drug resistance-associated phenotypes. Clin Cancer Res. 2004 Nov 1510(22):7599-606., URL: https://www.ncbi.nlm.nih.gov/pubmed/15569991,*Kato S, Ando K, Nakamura Y, Muguruma Y, Sato T, Yabe H, Yabe M, Hattori K, Yasuda Y, Hotta T: Absence of a CD34- hematopoietic precursor population in recipients of CD34+ stem cell transplantation. Bone Marrow Transplant. 2001 Sep28(6):587-95., URL: https://www.ncbi.nlm.nih.gov/pubmed/11607772,*Felschow DM, McVeigh ML, Hoehn GT, Civin CI, Fackler MJ: The adapter protein CrkL associates with CD34. Blood. 2001 Jun 1597(12):3768-75., URL: https://www.ncbi.nlm.nih.gov/pubmed/11389015,*Janowska-Wieczorek A, Marquez LA, Nabholtz JM, Cabuhat ML, Montaño J, Chang H, Rozmus J, Russell JA, Edwards DR, Turner AR: Growth factors and cytokines upregulate gelatinase expression in bone marrow CD34(+) cells and their transmigration through reconstituted basement membrane. Blood. 1999 May 1593(10):3379-90., URL: https://www.ncbi.nlm.nih.gov/pubmed/10233890,*Ando K, Nakamura Y, Chargui J, Matsuzawa H, Tsuji T, Kato S, Hotta T: Extensive generation of human cord blood CD34(+) stem cells from Lin(-)CD34(-) cells in a long-term in vitro system. Exp Hematol. 2000 Jun28(6):690-9., URL: https://www.ncbi.nlm.nih.gov/pubmed/10880755,*Bonelli P, Nicolussi P, Manetti R, Antuofermo E, Dattena M: Monoclonal antigen do not cross-react with ovine umbilical cord blood cells. Ital J Anim Sci 2010, vol.9:e6., URL: https://www.tandfonline.com/doi/full/10.4081/ijas.2010.e6,
Other References: *Krauter J, Hartl M, Hambach L, Kohlenberg A, Gunsilius E, Ganser A, Heil G: Receptor-mediated endocytosis of CD34 on hematopoietic cells after stimulation with the monoclonal antibody anti-HPCA-1. J Hematother Stem Cell Res. 2001 Dec10(6):863-71., URL: http://www.ncbi.nlm.nih.gov/pubmed/11798512?ordina,*Dao MA, Arevalo J, Nolta JA: Reversibility of CD34 expression on human hematopoietic stem cells that retain the capacity for secondary reconstitution. Blood. 2003 Jan 1101(1):112-8., URL: http://www.ncbi.nlm.nih.gov/pubmed/12393633?ordina,*Gangenahalli GU, Singh VK, Verma YK, Gupta P, Sharma RK, Chandra R, Gulati S, Luthra PM: Three-dimensional structure prediction of the interaction of CD34 with the SH3 domain of Crk-L. Stem Cells Dev. 2005 Oct14(5):470-7., URL: http://www.ncbi.nlm.nih.gov/pubmed/16305332?ordina,*Gangenahalli GU, Singh VK, Verma YK, Gupta P, Sharma RK, Chandra R, Luthra PM: Hematopoietic stem cell antigen CD34: role in adhesion or homing. Stem Cells Dev. 2006 Jun15(3):305-13., URL: http://www.ncbi.nlm.nih.gov/pubmed/16846369?ordina,
Storage Conditions: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

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1A-664-T025	Anti-Hu CD34 APC