Anti-FoxP3 PE

Anti-FoxP3 PE

• Background: FoxP3 (forkhead box protein 3), a highly conserved forkhead/winged-helix transcription factor, plays a crucial role in maintaining immune homeostasis by governing the development and function of regulatory T cells. It is constitutively expressed at high level in CD25+ CD4+ Treg cells and at low level in a CD25- CD4+ Treg cell subset. Defects in gene encoding FoxP3 protein cause the scurfy phenotype in mice, and in human the IPEX syndrome (immune dysfunction, polyendocrinopathy, enteropathy, X-linked syndrome), also known as X-linked autoimmunity-allergic dysregulation (XLAAD) syndrome.
• Specifications: The mouse monoclonal antibody 3G3 recognizes N-terminal region of FoxP3, a 47-55 kDa transcription factor (intracellular antigen), which is the master regulator in the development and function of regulatory T cells.
• Certification: RUO
• Host: Mouse
• Species Reactivity: Human, Mouse
• Immunogen: Full-length His-tagged recombinant murine FoxP3
• Target Antigen: FoxP3
• Isotype: Igg1 κ
• Clone: 3G3
• Applications: Flow cytometry: Recommended dilution: 1-5 μg/mL. Intracellular staining.
• Validated Applications: FC
• Concentration: 0.5 mg/mL
• Format: PE
• Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• References & Citations: *Law JP, Hirschkorn DF, Owen RE, Biswas HH, Norris PJ, Lanteri MC: The importance of Foxp3 antibody and fixation/permeabilization buffer combinations in identifying CD4+CD25+Foxp3+ regulatory T cells. Cytometry A. 2009 Dec75(12):1040-50., URL: https://www.ncbi.nlm.nih.gov/pubmed/19845018?itool,*Gavin MA, Torgerson TR, Houston E, DeRoos P, Ho WY, Stray-Pedersen A, Ocheltree EL, Greenberg PD, Ochs HD, Rudensky AY: Single-cell analysis of normal and FOXP3-mutant human T cells: FOXP3 expression without regulatory T cell development. Proc Natl Acad Sci U S A. 2006 Apr 25103(17):6659-64., URL: https://www.ncbi.nlm.nih.gov/pubmed/16617117?itool,
• Other References: *Kuhn A, Beissert S, Krammer PH: CD4(+)CD25 (+) regulatory T cells in human lupus erythematosus. Arch Dermatol Res. 2009 Jan301(1):71-81., URL: https://www.ncbi.nlm.nih.gov/pubmed/18985367?itool,*Lal G, Bromberg JS: Epigenetic mechanisms of regulation of Foxp3 expression. Blood. 2009 Oct 29114(18):3727-35. , URL: https://www.ncbi.nlm.nih.gov/pubmed/19641188,*Barnes MJ, Powrie F: Regulatory T cells reinforce intestinal homeostasis. Immunity. 2009 Sep 1831(3):401-11., URL: https://www.ncbi.nlm.nih.gov/pubmed/19766083?itool,*Bettini M, Vignali DA: Regulatory T cells and inhibitory cytokines in autoimmunity. Curr Opin Immunol. 2009 Dec21(6):612-8., URL: https://www.ncbi.nlm.nih.gov/pubmed/19854631?itool,*Elkord E: Novel therapeutic strategies by regulatory T cells in allergy. Chem Immunol Allergy. 200894:150-7., URL: https://www.ncbi.nlm.nih.gov/pubmed/18802345?itool,
• Storage Conditions: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.