Anti-Hu CD11c PE

• Description:

  CD11c (p150, alphaX integrin subunit) forms complex with CD18 (beta2 integrin subunit) and is expressed mainly on tissue macrophages and dendritic cells. CD11c binds to complement fragment iC3b, fibrinogen, VCAM-1 and ICAM-2 or e.g. CD90. Like other beta2 integrins, CD11c/CD18 plays roles in cell migration and phagocytosis. Moreover, interaction of CD11c/CD18 with plasminogen regulates plasmin activities, and interaction with heparin counteracts binding of iC3b.

• Certification:

  RUO

• Immunogen:

  Dendritic cells of synovial fluid

• Target Antigen:

  CD11c

• Clone:

  BU15

• Applications:

  FC (QC tested)

• Format:

  PE

• Buffer:

  Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

• References & Citations:

  *Van der Vieren M, Le Trong H, Wood CL, Moore PF, St John T, Staunton DE, Gallatin WM: A novel leukointegrin, alpha d beta 2, binds preferentially to ICAM-3. Immunity. 1995 Dec;3 (6) :683-90., URL: http://www.ncbi.nlm.nih.gov/pubmed/8777714?ordinal, *Myou S, Zhu X, Boetticher E, Qin Y, Myo S, Meliton A, Lambertino A, Munoz NM, Hamann KJ, Leff AR: Regulation of adhesion of AML14.3D10 cells by surface clustering of beta2-integrin caused by ERK-independent activation of cPLA2. Immunology. 2002 Sep;107 (1) :77-85., URL: http://www.ncbi.nlm.nih.gov/pubmed/12225365?ordina, *Sadhu C, Hendrickson L, Dick KO, Potter TG, Staunton DE: Novel Tools for Functional Analysis of CD11c: Activation-Specific, Activation-Independent, and Activating Antibodies. J Immunoassay Immunochem. 2008;29 (1) :42-57., URL: http://www.ncbi.nlm.nih.gov/pubmed/18080879?ordina, *Angel CE, Lala A, Chen CJ, Edgar SG, Ostrovsky LL, Dunbar PR: CD14+ antigen-presenting cells in human dermis are less mature than their CD1a+ counterparts. Int Immunol. 2007 Nov;19 (11) :1271-9., URL: http://www.ncbi.nlm.nih.gov/pubmed/17804688?itool=, *Rezzonico R, Imbert V, Chicheportiche R, Dayer JM: Ligation of CD11b and CD11c beta (2) integrins by antibodies or soluble CD23 induces macrophage inflammatory protein 1alpha (MIP-1alpha) and MIP-1beta production in primary human monocytes through a pathway dependent on nuclear factor-kappaB. Blood. 2001 May 15;97 (10) :2932-40., URL: http://www.ncbi.nlm.nih.gov/pubmed/11342414?ordina, *Leukocyte Typing III., McMichael A. J. et al (Eds.), Oxford University Press (1987) ., URL: , *Leukocyte Typing V., Schlossman S. et al. (Eds.), Oxford University Press (1995) ., URL: , *Leukocyte Typing VI., Kishimoto T. et al. (Eds.), Garland Publishing Inc. (1997) ., URL: , *Hogg N, Takacs L, Palmer DG, Selvendran Y, Allen C: The p150,95 molecule is a marker of human mononuclear phagocytes: comparison with expression of class II molecules. Eur J Immunol. 1986 Mar;16 (3) :240-8., URL: http://www.ncbi.nlm.nih.gov/pubmed/3456894?ordinal, *Trowald-Wigh G, Johannisson A, Hå kansson L: Canine neutrophil adhesion proteins and Fc-receptors in healthy dogs and dogs with adhesion protein deficiency, as studied by flow cytometry. Vet Immunol Immunopathol. 1993 Oct;38 (3-4) :297-310., URL: http://www.ncbi.nlm.nih.gov/pubmed/8291207?ordinal

• Other References:

  *Vorup-Jensen T, Chi L, Gjelstrup LC, Jensen UB, Jewett CA, Xie C, Shimaoka M, Linhardt RJ, Springer TA: Binding between the integrin alphaXbeta2 (CD11c/CD18) and heparin. J Biol Chem. 2007 Oct 19;282 (42) :30869-77., URL: http://www.ncbi.nlm.nih.gov/pubmed/17699512?ordina, *Gang J, Choi J, Lee JH, Nham SU: Identification of critical residues for plasminogen binding by the alphaXI-domain of the beta2 integrin, alphaXbeta2. Mol Cells. 2007 Oct 31;24 (2) :240-6., URL: http://www.ncbi.nlm.nih.gov/pubmed/17978577?ordina, *Sadhu C, Ting HJ, Lipsky B, Hensley K, Garcia-Martinez LF, Simon SI, Staunton DE: CD11c/CD18: novel ligands and a role in delayed-type hypersensitivity. J Leukoc Biol. 2007 Jun;81 (6) :1395-403., URL: http://www.ncbi.nlm.nih.gov/pubmed/17389580?ordina, *Bullard DC, Hu X, Adams JE, Schoeb TR, Barnum SR: p150/95 (CD11c/CD18) expression is required for the development of experimental autoimmune encephalomyelitis. Am J Pathol. 2007 Jun;170 (6) :2001-8., URL: http://www.ncbi.nlm.nih.gov/pubmed/17525267?ordina

• Storage Conditions:

  Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

• Specificity:

  The antibody BU15 reacts with an extracellular epitope of CD11c (alphaX, p150), a 150 kDa integrin expressed mainly on dendritic cells and tissue macrophages.

• Applications Notes:

  6

• Species:

  Human, Monkey

• CAS Number:

  9007-83-4

• Isotype:

  Mouse IgG1 kappa