Anti-Hu CD300a PE

CD300a (CMRF-35H, IRp60) is a non-MHC-specific inhibitory receptor of immunoglobulin superfamily, which contains three immunoreceptor tyrosine-based inhibitory motifs (ITIMs) that associate with SH2-containing phosphatases SHP-1 and SHP-2. CD300a is expressed on many cell types including T cells, NK cells, neutrophils, eosinophils or mast cells. Its triggering inhibits activating signals such as those of IL5, GM-CSF or eotaxin, as well as supresses mast cell degranulation or NK cell cytotoxic activity.

Human

HPB human acute lymphoid leukemia cell line

CD300a

MEM-260

FC (QC tested)

PE

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

*Carnec X, Meertens L, Dejarnac O, Perera-Lecoin M, Hafirassou ML, Kitaura J, Ramdasi R, Schwartz O, Amara A: The Phosphatidylserine and Phosphatidylethanolamine Receptor CD300a Binds Dengue Virus and Enhances Infection. J Virol. 2015 Oct 14;90 (1) :92-102. , URL: https://www.ncbi.nlm.nih.gov/pubmed/26468529 , *Sabato V, Verweij MM, Bridts CH, Levi-Schaffer F, Gibbs BF, De Clerck LS, Schiavino D, Ebo DG: CD300a is expressed on human basophils and seems to inhibit IgE/Fcε RI-dependent anaphylactic degranulation. Cytometry B Clin Cytom. 2012 May;82 (3) :132-8., URL: https://www.ncbi.nlm.nih.gov/pubmed/22173928

*Alvarez Y, Tang X, Coligan JE, Borrego F: The CD300a (IRp60) inhibitory receptor is rapidly up-regulated on human neutrophils in response to inflammatory stimuli and modulates CD32a (FcgammaRIIa) mediated signaling. Mol Immunol. 2008 Jan;45 (1) :253-8., URL: https://www.ncbi.nlm.nih.gov/pubmed/17588661?ordin, *Dimasi N, Roessle M, Moran O, Candiano G, Svergun DI, Biassoni R: Molecular analysis and solution structure from small-angle X-ray scattering of the human natural killer inhibitory receptor IRp60 (CD300a) . Int J Biol Macromol. 2007 Feb 20;40 (3) :193-200., URL: https://www.ncbi.nlm.nih.gov/pubmed/16949664?ordin, *Bachelet I, Munitz A, Levi-Schaffer F: Abrogation of allergic reactions by a bispecific antibody fragment linking IgE to CD300a. J Allergy Clin Immunol. 2006 Jun;117 (6) :1314-20., URL: https://www.ncbi.nlm.nih.gov/pubmed/16750992?ordin, *Bachelet I, Munitz A, Moretta A, Moretta L, Levi-Schaffer F: The inhibitory receptor IRp60 (CD300a) is expressed and functional on human mast cells. J Immunol. 2005 Dec 15;175 (12) :7989-95., URL: https://www.ncbi.nlm.nih.gov/pubmed/16339535?ordin, *Cantoni C, Bottino C, Augugliaro R, Morelli L, Marcenaro E, Castriconi R, Vitale M, Pende D, Sivori S, Millo R, Biassoni R, Moretta L, Moretta A: Molecular and functional characterization of IRp60, a member of the immunoglobulin superfamily that functions as an inhibitory receptor in human NK cells. Eur J Immunol. 1999 Oct;29 (10) :3148-59., URL: https://www.ncbi.nlm.nih.gov/pubmed/10540326?ordin

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

The antibody MEM-260 reacts with an extracellular epitope of CD300a, a 60 kDa leukocyte transmembrane glycoprotein expressed on human granulocytes, monocytes, neutrophils, NK cells, mast cells and dendritic cells, 25% of circulating T cells and 15% of circulating B cells.

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