Anti-Ikaros PE

Anti-Ikaros PE

• Background: Ikaros, also known as IKZF1 (Ikaros family zinc finger protein 1) is a hematopoietic-specific transcription factor involved in the regulation of lymphocyte development, together with other members of this family, such as Aiolos and Helios. Ikaros forms homo- and heterodimers with these proteins and functions predominantly in early hematopoietic development. Expression of Ikaros, Aiolos and Helios is restricted to cells of the hematopoietic system, whereas other family members, Eos and Pegassus, are more widely expressed. Disruption of Ikaros leads to T and B cell leukemias.
• Specifications: The mouse monoclonal antibody 4E9 recognizes Ikaros, a transcription factor (intracellular antigen) expressed broadly in hematopoietic progenitors and serving as a key regulator of lymphopoiesis.
• Certification: RUO
• Host: Mouse
• Species Reactivity: Mouse, Human
• Immunogen: Recombinant human Ikaros (C-terminal part)
• Target Antigen: Ikaros
• Isotype: IgG1
• Clone: 4E9
• Applications: Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (1 mL) is sufficient for 100 tests. Intracellular staining.
• Validated Applications: FC
• Format: PE
• Buffer: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• References & Citations: *López-Nieva P, Santos J, Fernández-Piqueras J: Defective expression of Notch1 and Notch2 in connection to alterations of c-Myc and Ikaros in gamma-radiation-induced mouse thymic lymphomas. Carcinogenesis. 2004 Jul25(7):1299-304. , URL: http://www.ncbi.nlm.nih.gov/pubmed/14976135 ,*Loeper S, Asa SL, Ezzat S: Ikaros modulates cholesterol uptake: a link between tumor suppression and differentiation. Cancer Res. 2008 May 1568(10):3715-23., URL: http://www.ncbi.nlm.nih.gov/pubmed/18483254 ,*Kathrein KL, Lorenz R, Innes AM, Griffiths E, Winandy S: Ikaros induces quiescence and T-cell differentiation in a leukemia cell line. Mol Cell Biol. 2005 Mar25(5):1645-54., URL: https://pubmed.ncbi.nlm.nih.gov/15713624/,*Schwickert TA, Tagoh H, Gültekin S, Dakic A, Axelsson E, Minnich M, Ebert A, Werner B, Roth M, Cimmino L, Dickins RA, Zuber J, Jaritz M, Busslinger M: Stage-specific control of early B cell development by the transcription factor Ikaros. Nat Immunol. 2014 Mar15(3):283-93., URL: http://www.ncbi.nlm.nih.gov/pubmed/24509509 ,*Elliott J, Jolicoeur C, Ramamurthy V, Cayouette M: Ikaros confers early temporal competence to mouse retinal progenitor cells. Neuron. 2008 Oct 960(1):26-39., URL: http://www.ncbi.nlm.nih.gov/pubmed/18940586 ,*Koipally J, Heller EJ, Seavitt JR, Georgopoulos K: Unconventional potentiation of gene expression by Ikaros. J Biol Chem. 2002 Apr 12277(15):13007-15., URL: http://www.ncbi.nlm.nih.gov/pubmed/?term=koipally+j+heller+ej,
• Storage Conditions: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.