Anti-Ki-67 PE

Anti-Ki-67 PE

• Background: Ki-67 is a highly protease-sensitive nuclear protein expressed in two isoforms (345 kDa and 395 kDa), both of which are identified by the antibody clone Ki-67. The Ki-67 antigen is essential for cell proliferation and its expression is restricted to the cycling cells. It is detected in G1, S, G2 and M phase, whereas it is absent in cells which are in G0 phase and it is not associated with DNA repair processes. Ki-67 thus represents an important tool for detection of proliferating cells, which is of great importance in tumor diagnostics and is commonly used as a prognostic factor in cancer studies.
• Specifications: The mouse monoclonal antibody Ki-67 recognizes Ki-67 antigen, a non-histone nuclear protein expressed exclusively in proliferating cells.
• CAS Number: 9007-83-4
• Certification: RUO
• Host: Mouse
• Species Reactivity: Cow, Human
• Immunogen: Nuclei of the Hodgkin lymphoma cell line L428
• Target Antigen: Ki-67
• Isotype: IgG1
• Clone: Ki-67
• Applications: Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (1 mL) is sufficient for 100 tests. Intracellular staining.
• Validated Applications: FC
• Format: PE
• Buffer: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• References & Citations: *Schlüter C, Duchrow M, Wohlenberg C, Becker MH, Key G, Flad HD, Gerdes J: The cell proliferation-associated antigen of antibody Ki-67: a very large, ubiquitous nuclear protein with numerous repeated elements, representing a new kind of cell cycle-maintaining proteins. J Cell Biol. 1993 Nov123(3):513-22., URL: http://www.ncbi.nlm.nih.gov/pubmed/8227122 ,*Duchrow M, Schlüter C, Key G, Kubbutat MH, Wohlenberg C, Flad HD, Gerdes J: Cell proliferation-associated nuclear antigen defined by antibody Ki-67: a new kind of cell cycle-maintaining proteins. Arch Immunol Ther Exp (Warsz). 199543(2):117-21., URL: https://www.ncbi.nlm.nih.gov/pubmed/8744726,*Gerdes J, Lemke H, Baisch H, Wacker HH, Schwab U, Stein H: Cell cycle analysis of a cell proliferation-associated human nuclear antigen defined by the monoclonal antibody Ki-67. J Immunol. 1984 Oct133(4):1710-5., URL: https://pubmed.ncbi.nlm.nih.gov/6206131/,*Gerdes J: Ki-67 and other proliferation markers useful for immunohistological diagnostic and prognostic evaluations in human malignancies. Semin Cancer Biol. 1990 Jun1(3):199-206., URL: https://www.ncbi.nlm.nih.gov/pubmed/2103495,*Gerdes J, Schwab U, Lemke H, Stein H: Production of a mouse monoclonal antibody reactive with a human nuclear antigen associated with cell proliferation. Int J Cancer. 1983 Jan 1531(1):13-20., URL: https://www.ncbi.nlm.nih.gov/pubmed/6339421,*Bading H, Rauterberg EW, Moelling K: Distribution of c-myc, c-myb, and Ki-67 antigens in interphase and mitotic human cells evidenced by immunofluorescence staining technique. Exp Cell Res. 1989 Nov185(1):50-9., URL: https://pubmed.ncbi.nlm.nih.gov/2680541/,
• Storage Conditions: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.