Anti-Hu CD142 PE

CD142, also known as coagulation factor III, tissue thromboplastin, and tissue factor. It is a transmembrane glycoprotein, which enables cells to initiate the blood coagulation cascades, and functions as the high-affinity receptor for the coagulation factor VII. The resulting complex provides a catalytic event that is responsible for initiation of the coagulation protease cascades by specific limited proteolysis. Unlike the other cofactors of these protease cascades, which circulate as nonfunctional precursors, this factor is a potent initiator that is fully functional when expressed on cell surfaces. It is the only one factor in the coagulation pathway for which a congenital deficiency has not been described.

Human

Human brain tissue factor (CD142)

CD142

HTF-1

FC (QC tested)

PE

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

*Bucciarelli P, Martinelli I, Artoni A, Passamonti SM, Previtali E, Merati G, Tripodi A, Mannucci PM: Circulating microparticles and risk of venous thromboembolism. Thromb Res. 2012 May;129 (5) :591-7, URL: https://www.ncbi.nlm.nih.gov/pubmed/21908018, *Patil R, Ghosh K, Satoskar P, Shetty S: Elevated procoagulant endothelial and tissue factor expressing microparticles in women with recurrent pregnancy loss. PLoS One. 2013 Nov 20;8 (11) :e81407., URL: https://pubmed.ncbi.nlm.nih.gov/24278436/, *Aass HC, Øvstebø R, Trøseid AM, Kierulf P, Berg JP, Henriksson CE: Fluorescent particles in the antibody solution result in false TF- and CD14-positive microparticles in flow cytometric analysis. Cytometry A. 2011 Dec;79 (12) :990-9. doi: 10.1002/cyto.a.21147., URL: https://www.ncbi.nlm.nih.gov/pubmed/21990118, *Carson SD, Ross SE, Bach R, Guha A: An inhibitory monoclonal antibody against human tissue factor. Blood. 1987 Aug;70 (2) :490-3., URL: https://www.ncbi.nlm.nih.gov/pubmed/3607285, *Faulk WP, Labarrere CA, Carson SD: Tissue factor: Identification and characterization of cell types in human placentae. Volume 76, Issue 1, 1 July 1990, Pages 86-96., URL: https://www.sciencedirect.com/science/article/pii/S0006497120842633, *Virtanen S, Schulte R, Stingl J, Caldas C, Shehata M: High-throughput surface marker screen on primary human breast tissues reveals further cellular heterogeneity. Breast Cancer Res. 2021 Jun 13;23 (1) :66., URL: https://pubmed.ncbi.nlm.nih.gov/34120626/

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

The mouse monoclonal antibody HTF-1, also known as HTF1-7B8, recognizes an extracellular epitope of CD142 (tissue factor, coagulation factor III), a type I glycoprotein expressed on endothelial cells, monocytes, macrophages, and platelets upon induction by inflammatory mediators, and expressed constitutively by some tumors, the vasculature, placenta, kidney, and central nervous system.

6