Anti-Hu CD42a FITC

• Description:

  CD42a, also known as glycoprotein 9 (GPIX), composes together with GPIb alpha, GPIb beta and GPV the GPIb-IX-V receptor complex critical in the process of platelet-rich thrombus formation by tethering the platelet to a thrombogenic surface. CD42b binds to von Willebrand factor (VWF) exposed at a site of vascular injury, as well as to thrombin, coagulation factors XI and XII, high molecular wight kininogen, TSP-1, integrin Mac-1 and P-selectin. Defects in the gene encoding CD42a are a cause of Bernard-Soulier syndrome, also known as giant platelet disease. These patients have unusually large platelets and have a clinical bleeding tendency.

• Certification:

  RUO

• Immunogen:

  Human acute lymphoblastic leukemia cells

• Target Antigen:

  CD42a

• Clone:

  GR-P

• Applications:

  FC (QC tested)

• Format:

  FITC

• Buffer:

  Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

• References & Citations:

  *Brown SB, Clarke MC, Magowan L, Sanderson H, Savill J: Constitutive death of platelets leading to scavenger receptor-mediated phagocytosis. A caspase-independent cell clearance program. J Biol Chem. 2000 Feb 25;275 (8) :5987-96., URL: http://www.ncbi.nlm.nih.gov/pubmed/?term=brown+sb+clarke+mc+magowan+l , *Vettore S, Scandellari R, Moro S, Lombardi AM, Scapin M, Randi ML, Fabris F: Novel point mutation in a leucine-rich repeat of the GPIbalpha chain of the platelet von Willebrand factor receptor, GPIb/IX/V, resulting in an inherited dominant form of Bernard-Soulier syndrome affecting two unrelated families: the N41H variant. Haematologica. 2008 Nov;93 (11) :1743-7., URL: http://www.ncbi.nlm.nih.gov/pubmed/18815197 , *Din JN, Aftab SM, Jubb AW, Carnegy FH, Lyall K, Sarma J, Newby DE, Flapan AD: Effect of moderate walnut consumption on lipid profile, arterial stiffness and platelet activation in humans. Eur J Clin Nutr. 2011 Feb;65 (2) :234-9., URL: http://www.ncbi.nlm.nih.gov/pubmed/?term=carnegy+fh+lyall+k , *Harding SA, Din JN, Sarma J, Josephs DH, Fox KA, Newby DE: Promotion of proinflammatory interactions between platelets and monocytes by unfractionated heparin. Heart. 2006 Nov;92 (11) :1635-8., URL: http://www.ncbi.nlm.nih.gov/pubmed/16709700

• Storage Conditions:

  Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

• Specificity:

  The mouse monoclonal antibody GR-P (also known as GRP-P) recognizes an extracellular epitope of CD42a (glycoprotein 9), a 22 kDa transmembrane protein constitutively expressed on megakaryocytes and platelets.

• Applications Notes:

  6

• Species:

  Dog, Human

• CAS Number:

  9007-83-4

• Isotype:

  Mouse IgG1