Anti-Hu CD19 FITC

Anti-Hu CD19 FITC

• Background: CD19 is a transmembrane glycoprotein of Ig superfamily expressed by B cells from the time of heavy chain rearrangement until plasma cell differentiation. It forms a tetrameric complex with CD21 (complement receptor type 2), CD81 (TAPA-1) and Leu13. Together with BCR (B cell antigen receptor), this complex signals to decrease B cell treshold for activation by the antigen. Besides being signal-amplifying coreceptor for BCR, CD19 can also signal independently of BCR coligation and it turns out to be a central regulatory component upon which multiple signaling pathways converge. Mutation of the CD19 gene results in hypogammaglobulinemia, whereas CD19 overexpression causes B cell hyperactivity.
• Specifications: The mouse monoclonal antibody 4G7 recognizes an extracellular epitope of human CD19.
• Certification: RUO
• Host: Mouse
• Species Reactivity: Human
• Immunogen: Human CCL (chronic lymphocytic leukemia) cells
• Target Antigen: CD19
• Isotype: Igg1 κ
• Clone: 4G7
• Applications: Flow cytometry: The reagent is designed for analysis of human blood cells using 20 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (2 mL) is sufficient for 100 tests.
• Validated Applications: FC
• Format: FITC
• Buffer: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• References & Citations: *Muench MO, Roncarolo MG, Namikawa R: Phenotypic and functional evidence for the expression of CD4 by hematopoietic stem cells isolated from human fetal liver. Blood. 1997 Feb 1589(4):1364-75., URL: http://www.ncbi.nlm.nih.gov/pubmed/9028960,*Stockmeyer B, Dechant M, van Egmond M, Tutt AL, Sundarapandiyan K, Graziano RF, Repp R, Kalden JR, Gramatzki M, Glennie MJ, van de Winkel JG, Valerius T: Triggering Fc alpha-receptor I (CD89) recruits neutrophils as effector cells for CD20-directed antibody therapy. J Immunol. 2000 Nov 15165(10):5954-61., URL: http://www.ncbi.nlm.nih.gov/pubmed/11067958 ,*Dubois B, Massacrier C, Caux C: Selective attraction of naive and memory B cells by dendritic cells. J Leukoc Biol. 2001 Oct70(4):633-41. , URL: http://www.ncbi.nlm.nih.gov/pubmed/11590201 ,*Basu S, Lynne CM, Ruiz P, Aballa TC, Ferrell SM, Brackett NL: Cytofluorographic identification of activated T-cell subpopulations in the semen of men with spinal cord injuries. J Androl. 2002 Jul-Aug23(4):551-6., URL: http://www.ncbi.nlm.nih.gov/pubmed/12065463 ,*Köller M, Zwölfer B, Steiner G, Smolen JS, Scheinecker C: Phenotypic and functional deficiencies of monocyte-derived dendritic cells in systemic lupus erythematosus (SLE) patients. Int Immunol. 2004 Nov16(11):1595-604. , URL: http://www.ncbi.nlm.nih.gov/pubmed/15381672 ,*Treusch M, Vonthein R, Baur M, Günaydin I, Koch S, Stübiger N, Eckstein AK, Peter HH, Ness T, Zierhut M, Kötter I: Influence of human recombinant interferon-alpha2a (rhIFN-alpha2a) on altered lymphocyte subpopulations and monocytes in Behcet's disease. Rheumatology (Oxford). 2004 Oct43(10):1275-82., URL: http://www.ncbi.nlm.nih.gov/pubmed/15252211 ,*Porcellini S, Vallanti G, Nozza S, Poli G, Lazzarin A, Tambussi G, Siccardi AG, Grassi F: Improved thymopoietic potential in aviremic HIV infected individuals treated with HAART by intermittent IL-2 administration. AIDS. 2003 Jul 2517(11):1621-30., URL: http://www.ncbi.nlm.nih.gov/pubmed/12853744 ,*Andersen P, Pedersen MW, Woetmann A, Villingsh248j M, Stockhausen MT, Odum N, Poulsen HS: EGFR induces expression of IRF-1 via STAT1 and STAT3 activation leading to growth arrest of human cancer cells. Int J Cancer. 2008 Jan 15122(2):342-9., URL: http://www.ncbi.nlm.nih.gov/pubmed/17918184 ,*Martino V, Tonelli R, Montemurro L, Franzoni M, Marino F, Fazzina R, Pession A: Down-regulation of MLL-AF9, MLL and MYC expression is not obligatory for monocyte-macrophage maturation in AML-M5 cell lines carrying t(911)(p22q23). Oncol Rep. 2006 Jan15(1):207-11., URL: http://www.ncbi.nlm.nih.gov/pubmed/16328057 ,*Leukocyte Typing II., Reinherz E.L. et al. (Eds.), Springer-Verlag (1985)., URL: ,*Mueller GA, Smith AM, Chapman MD, Rule GS, Benjamin DC: Hydrogen exchange nuclear magnetic resonance spectroscopy mapping of antibody epitopes on the house dust mite allergen Der p 2. J Biol Chem. 2001 Mar 23276(12):9359-65., URL: http://www.ncbi.nlm.nih.gov/pubmed/11134039,*Smith AM, Benjamin DC, Hozic N, Derewenda U, Smith WA, Thomas WR, Gafvelin G, van Hage-Hamsten M, Chapman MD: The molecular basis of antigenic cross-reactivity between the group 2 mite allergens. J Allergy Clin Immunol. 2001 Jun107(6):977-84., URL: http://www.ncbi.nlm.nih.gov/pubmed/11398074 ,
• Other References: *Shi X, Xie C, Chang S, Zhou XJ, Tedder T, Mohan C: CD19 hyperexpression augments Sle1-induced humoral autoimmunity but not clinical nephritis. Arthritis Rheum. 2007 Sep56(9):3057-69., URL: https://pubmed.ncbi.nlm.nih.gov/17763445/,*van Zelm MC, Reisli I, van der Burg M, Castaño D, van Noesel CJ, van Tol MJ, Woellner C, Grimbacher B, Patiño PJ, van Dongen JJ, Franco JL: An antibody-deficiency syndrome due to mutations in the CD19 gene. N Engl J Med. 2006 May 4354(18):1901-12., URL: https://pubmed.ncbi.nlm.nih.gov/16672701/,*Inabe K, Kurosaki T: Tyrosine phosphorylation of B-cell adaptor for phosphoinositide 3-kinase is required for Akt activation in response to CD19 engagement. Blood. 2002 Jan 1599(2):584-9., URL: https://pubmed.ncbi.nlm.nih.gov/11781242/,*Fujimoto M, Poe JC, Jansen PJ, Sato S, Tedder TF: CD19 amplifies B lymphocyte signal transduction by regulating Src-family protein tyrosine kinase activation. J Immunol. 1999 Jun 15162(12):7088-94., URL: https://pubmed.ncbi.nlm.nih.gov/10358152/,
• Storage Conditions: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.