Anti-Hu TCR alpha/beta FITC

• Description:

  The antigen-specific T cell receptor (TCR) is composed of either alpha and beta subunit, or gamma and delta subunit. Majority of T cells present in the blood, lymph and secondary lymphoid organs express TCR alpha/beta heterodimers, whereas the T cells expressing TCR gamma/delta heterodimers are localized mainly in epithelial tissues and at the sites of infection. The subunits of TCR heterodimers are covalently bonded and in the endoplasmic reticulum they associate with CD3 subunits to form functional TCR-CD3 complex. Lack of expression of any of the chains is sufficient to stop cell surface expression.

• Certification:

  RUO

• Target Antigen:

  TCR alpha/beta

• Clone:

  IP26

• Applications:

  FC (QC tested)

• Format:

  FITC

• Buffer:

  Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

• References & Citations:

  *Kuttruff S, Koch S, Kelp A, Pawelec G, Rammensee HG, Steinle A: NKp80 defines and stimulates a reactive subset of CD8 T cells. Blood. 2009 Jan 8;113 (2) :358-69., URL: http://www.ncbi.nlm.nih.gov/pubmed/18922855 Sulliv

• Other References:

  *von Boehmer H: T cell development and selection in the thymus. Bone Marrow Transplant. 1992;9 Suppl 1:46-8. , URL: https://www.ncbi.nlm.nih.gov/pubmed/1324045?ordina, *Levelt CN, Wang B, Ehrfeld A, Terhorst C, Eichmann K: Regulation of T cell receptor (TCR) -beta locus allelic exclusion and initiation of TCR-alpha locus rearrangement in immature thymocytes by signaling through the CD3 complex. Eur J Immunol. 1995 May;25 (5) :1257-61., URL: https://www.ncbi.nlm.nih.gov/pubmed/7774628?ordina, *Michie AM, Zúñiga-Pflücker JC: Regulation of thymocyte differentiation: pre-TCR signals and beta-selection. Semin Immunol. 2002 Oct;14 (5) :311-23., URL: https://www.ncbi.nlm.nih.gov/pubmed/12220932?ordin, *Croxford AL, Akilli-Ozturk O, Rieux-Laucat F, Förster I, Waisman A, Buch T: MHC-restricted T cell receptor signaling is required for alpha beta TCR replacement of the pre T cell receptor. Eur J Immunol. 2008 Feb;38 (2) :391-9., URL: https://www.ncbi.nlm.nih.gov/pubmed/18203137?ordin

• Storage Conditions:

  Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

• Specificity:

  The mouse monoclonal antibody IP26 recognizes a monomorphic extracellular determinant of TCR alpha/beta, the dominant subtype of T cell receptor expressed in human peripheral blood.

• Applications Notes:

  6

• Species:

  Human

• CAS Number:

  9007-83-4

• Isotype:

  Mouse IgG1