Anti-c-Myc tag FITC

Anti-c-Myc tag FITC

• Background: The c-myc gene (8q24 on human chromosome) is the cellular homologue of the v-myc gene originally isolated from an avian myelocytomatosis virus. The c-Myc protein is a transcription factor (nuclear localization). c-Myc is commonly activated in a variety of tumor cells and plays an important role in cellular proliferation, differentiation, apoptosis and cell cycle progression. The phosphorylation of c-Myc has been investigated and previous studies have suggested a functional association between phosphorylation at Thr58/Ser62 by glycogen synthase kinase 3, cyclin-dependent kinase, ERK2 and C-Jun N-terminal Kinase (JNK) in cell proliferation and cell cycle regulation. In normal cells the expression of c-Myc is tightly regulated but in human cancers c-Myc is frequently deregulated. c-Myc is also essential for tumor cell development in vasculogenesis and angiogenesis that distribute blood throughout the cells.
• Specifications: The antibody 9E10 can be used to detect the c-Myc tag.
• CAS Number: 9007-83-4
• Certification: RUO
• Host: Mouse
• Species Reactivity: Tagged fusion proteins in all species, Human
• Immunogen: Synthetic peptide sequence (AEEQKLISEEDLL) corresponding to the C-terminal region of human c-Myc.
• Target Antigen: c-Myc tag
• Isotype: IgG1
• Clone: 9E10
• Applications: Flow cytometry: Intracellular or extracellular staining, depending on particular expression. Recommended dilution: 1-5 μg/mL.
• Validated Applications: FC-IC, FC
• Concentration: 1 mg/mL
• Format: FITC
• Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• References & Citations: *Wang X, Campoli M, Ko E, Luo W, Ferrone S: Enhancement of scFv fragment reactivity with target antigens in binding assays following mixing with anti-tag monoclonal antibodies. J Immunol Methods. 2004 Nov294(1-2):23-35., URL: https://www.ncbi.nlm.nih.gov/pubmed/15604013?dopt=,*Hilpert K, Hansen G, Wessner H, Kuttner G, Welfle K, Seifert M, Hohne W: Anti-c-myc antibody 9E10: epitope key positions and variability characterized using peptide spot synthesis on cellulose. Protein Eng. 2001 Oct14(10):803-6., URL: https://pubmed.ncbi.nlm.nih.gov/11739900/,*Cermák L, Símová S, Pintzas A, Horejsí V, Andera L: Molecular mechanisms involved in CD43-mediated apoptosis of TF-1 cells. Roles of transcription Daxx expression, and adhesion molecules. J Biol Chem. 2002 Mar 8277(10):7955-61., URL: http://www.ncbi.nlm.nih.gov/pubmed?term=%C4%8Derm%C3%A1k%20l%20%C5%A1%C3%ADmov%C3%A1%20%C5%A1%20pintzas%20a ,*Jelínková I, Šafaříková B, Vondálová Blanařová O, Skender B, Hofmanová J, Sova P, Moyer MP, Kozubík A, Kolář Z, Ehrmann J, Hyršlová Vaculová A: Platinum(IV) complex LA-12 exerts higher ability than cisplatin to enhance TRAIL-induced cancer cell apoptosis via stimulation of mitochondrial pathway. Biochem Pharmacol. 2014 Dec 192(3):415-24., URL: https://pubmed.ncbi.nlm.nih.gov/25285768/,*Siegel J, Brandner G, Hess RD: Cross-reactivity of the monoclonal antibody 9E10 with murine c-MYC. Int J Oncol. 1998 Dec13(6):1259-62., URL: https://pubmed.ncbi.nlm.nih.gov/9824641/,*Veracini L, Simon V, Richard V, Schraven B, Horejsi V, Roche S, Benistant C: The Csk-binding protein PAG regulates PDGF-induced Src mitogenic signaling via GM1. J Cell Biol. 2008 Aug 11182(3):603-14., URL: http://www.ncbi.nlm.nih.gov/pubmed/18695048?itool=,*Baggio R, Burgstaller P, Hale SP, Putney AR, Lane M, Lipovsek D, Wright MC, Roberts RW, Liu R, Szostak JW, Wagner RW: Identification of epitope-like consensus motifs using mRNA display. J Mol Recognit. 2002 May-Jun15(3):126-34., URL: https://www.ncbi.nlm.nih.gov/pubmed/12203838?dopt=,*Fujiwara K, Poikonen K, Aleman L, Valtavaara M, Saksela K, Mayer BJ: A single-chain antibody/epitope system for functional analysis of protein-protein interactions. Biochemistry. 2002 Oct 2241(42):12729-38., URL: https://www.ncbi.nlm.nih.gov/pubmed/12379115?dopt=,*Svadlenka J, Brazina J, Hanzlikova H, Cermak L, Andera L: Multifunctional adaptor protein Daxx interacts with chromatin-remodelling ATPase Brg1. Biochem Biophys Rep. 2015 Dec 305:246-252., URL: https://www.ncbi.nlm.nih.gov/pubmed/28955830,
• Other References: *Persson H, Hennighausen L, Taub R, DeGrado W, Leder P: Antibodies to human c-myc oncogene product: evidence of an evolutionarily conserved protein induced during cell proliferation. Science. 1984 Aug 17225(4663):687-93. , URL: https://www.ncbi.nlm.nih.gov/pubmed/6431612?dopt=A,*Evan GI et al. 1985. Isolation of monoclonal antibodies specific for human c-myc proto-oncogene product. Mol Cell Biol 5:3610-6., URL: ,*Spandidos DA et al. 1987. Elevated expression of the myc gene in human benign and malignant breast lesions compared to normal tissue. Anticancer Res 7:1299-304., URL: ,*Prendergast GC. 1999. Mechanisms of apoptosis by c-Myc. Oncogene 18(19):2967-2987., URL: ,*Dang CV, Resar LM, Emison E, Kim S, Li Q, Prescott JE, Wonsey D, Zeller K. 1999. Function of the c-Myc oncogenic transcription factor. Exp Cell Res 253(1): 63-77., URL: ,*Boxer LM, Dang CV. 2001. Translocations involving c-myc and c-myc function. Oncogene 20(40):5595-5610., URL: ,*Hoffman B, Amanullah A, Shafarenko M, Liebermann DA. 2002. The proto-oncogene c-myc in hematopoietic development and leukemogenesis. Oncogene 21(21): 3414-3421., URL: ,
• Storage Conditions: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.