Anti-Hu CD20 FITC

• Clone:

  LT20

• Format:

  FITC

• Buffer:

  Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

• References & Citations:

  *Všianská P, Říhová L, Varmužová T, Suská R, Kryukov F, Mikulášová A, Kupská R, Penka M, Pour L, Adam Z, Hájek R: Analysis of B-cell subpopulations in monoclonal gammopathies. Clin Lymphoma Myeloma Leuk. 2015 Apr;15 (4) :e61-71., URL: https://www.ncbi.nlm.nih.gov/pubmed/25578543, *Filatov AV, Krotov GI, Zgoda VG, Volkov Y: Fluorescent immunoprecipitation analysis of cell surface proteins: a methodology compatible with mass-spectrometry. J Immunol Methods. 2007 Jan 30;319 (1-2) :21-33., URL: http://www.ncbi.nlm.nih.gov/pubmed/17098248 , *Kanderova V, Kuzilkova D, Stuchly J, Vaskova M, Brdicka T, Fiser K, Hrusak O, Lund-Johansen F, Kalina T: High-resolution Antibody Array Analysis of Childhood Acute Leukemia Cells. Mol Cell Proteomics. 2016 Apr;15 (4) :1246-61., URL: http://www.ncbi.nlm.nih.gov/pubmed/26785729 Všians, *Leukocyte Typing VII., Mason D. et al. (Eds.), Oxford University Press (2002) ., URL: , *Polyak MJ, Deans JP: Alanine-170 and proline-172 are critical determinants for extracellular CD20 epitopes; heterogeneity in the fine specificity of CD20 monoclonal antibodies is defined by additional requirements imposed by both amino acid sequence and quaternary structure. Blood. 2002 May 1;99 (9) :3256-62., URL: https://www.ncbi.nlm.nih.gov/pubmed/11964291?dopt=, *Chan HT, Hughes D, French RR, Tutt AL, Walshe CA, Teeling JL, Glennie MJ, Cragg MS: CD20-induced lymphoma cell death is independent of both caspases and its redistribution into triton X-100 insoluble membrane rafts. Cancer Res. 2003 Sep 1;63 (17) :5480-9., URL: https://www.ncbi.nlm.nih.gov/pubmed/14500384?dopt=, *Teeling JL, Mackus WJ, Wiegman LJ, van den Brakel JH, Beers SA, French RR, van Meerten T, Ebeling S, Vink T, Slootstra JW, Parren PW, Glennie MJ, van de Winkel JG: The biological activity of human CD20 monoclonal antibodies is linked to unique epitopes on CD20. J Immunol. 2006 Jul 1;177 (1) :362-71. , URL: https://www.ncbi.nlm.nih.gov/pubmed/16785532?dopt=

• Other References:

  *Li H, Ayer LM, Polyak MJ, Mutch CM, Petrie RJ, Gauthier L, Shariat N, Hendzel MJ, Shaw AR, Patel KD, Deans JP. The CD20 calcium channel is localized to microvilli and constitutively associated with membrane rafts: antibody binding increases the affinity of the association through an epitope-dependent cross-linking-independent mechanism. J Biol Chem. 2004 May 7;279 (19) :19893-901., URL: https://pubmed.ncbi.nlm.nih.gov/17497959/, *Glennie MJ, French RR, Cragg MS, Taylor RP: Mechanisms of killing by anti-CD20 monoclonal antibodies. Mol Immunol. 2007 Sep;44 (16) :3823-37., URL: https://pubmed.ncbi.nlm.nih.gov/17768100/, *Cragg MS, Walshe CA, Ivanov AO, Glennie MJ: The biology of CD20 and its potential as a target for mAb therapy. Curr Dir Autoimmun. 2005;8:140-74., URL: https://pubmed.ncbi.nlm.nih.gov/15564720/, *Petrie RJ, Deans JP: Colocalization of the B cell receptor and CD20 followed by activation-dependent dissociation in distinct lipid rafts. J Immunol. 2002 Sep 15;169 (6) :2886-91., URL: https://pubmed.ncbi.nlm.nih.gov/12218101/, *Hultin LE, Hausner MA, Hultin PM, Giorgi JV: CD20 (pan-B cell) antigen is expressed at a low level on a subpopulation of human T lymphocytes. Cytometry. 1993;14 (2) :196-204., URL: https://www.ncbi.nlm.nih.gov/pubmed/7679964?dopt=A

• CAS Number:

  9007-83-4