Anti-Hu CD58 FITC

• Description:

  CD58 (LFA-3) is an immunoglobulin family adhession molecule expressed by both hematopoietic and non-hematopoietic cells (often on antigen presenting cells) and serving as ligand of CD2. This interaction is important for T cell-mediated immunity. CD58 is expressed in transmembrane form and in GPI-anchored form; the later is constitutively associated with protein kinases whereas the transmembrane form activates kinase activity upon triggering. CD58 is a powerful tool for detection of minimal residual disease in acute lymphocytic leukemia, and for evaluation of liver damage related with hepatitis B.

• Certification:

  RUO

• Reactivity:

  Pig, Human

• Immunogen:

  NALM-6 human pre-B cell line

• Target Antigen:

  CD58

• Clone:

  MEM-63

• Applications:

  FC (QC tested)

• Format:

  FITC

• Buffer:

  Stabilizing Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide

• Other References:

  *Sheng L, Li J, Qi BT, Ji YQ, Meng ZJ, Xie M: Investigation on correlation between expression of CD58 molecule and severity of hepatitis B. World J Gastroenterol. 2006 Jul 14;12 (26) :4237-40., URL: https://www.ncbi.nlm.nih.gov/pubmed/16830383?ordin, *Ariel O, Kukulansky T, Raz N, Hollander N: Distinct membrane localization and kinase association of the two isoforms of CD58. Cell Signal. 2004 Jun;16 (6) :667-73., URL: https://www.ncbi.nlm.nih.gov/pubmed/15093607?ordin, *Veltroni M, De Zen L, Sanzari MC, Maglia O, Dworzak MN, Ratei R, Biondi A, Basso G, Gaipa G; I-BFM-ALL-FCM-MRD-Study Group: Expression of CD58 in normal, regenerating and leukemic bone marrow B cells: implications for the detection of minimal residual disease in acute lymphocytic leukemia. Haematologica. 2003 Nov;88 (11) :1245-52., URL: https://www.ncbi.nlm.nih.gov/pubmed/14607753?ordin, *Bayas MV, Schulten K, Leckband D: Forced detachment of the CD2-CD58 complex. Biophys J. 2003 Apr;84 (4) :2223-33., URL: https://www.ncbi.nlm.nih.gov/pubmed/12668431?ordin

• Storage Conditions:

  Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

• Specificity:

  The antibody MEM-63 reacts with CD58 (LFA-3), a 40-70 kDa extracellular membrane glycoprotein distributed over many tissues, leukocytes, erythrocytes, endothelial cells, epithelial cells and fibroblasts.

• Applications Notes:

  6

• Isotype:

  Mouse IgG1