Anti-Hu CD45 FITC

Anti-Hu CD45 FITC

• Background: CD45 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase ubiquitously expressed in all nucleated hematopoietic cells, comprising approximately 10% of all surface proteins in lymphocytes. CD45 glycoprotein is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases. CD45 protein exists as multiple isoforms as a result of alternative splicing these isoforms differ in their extracellular domains, whereas they share identical transmembrane and cytoplasmic domains. These isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. Besides the role in immunoreceptor signaling, CD45 is important in promoting cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis.
• Specifications: The antibody MEM-28 reacts with an extracellular epitope on all alternative forms of human CD45 antigen (Leukocyte Common Antigen), a 180-220 kDa single chain type I transmembrane protein expressed at high level on all cells of hematopoietic origin, except erythrocytes and platelets.
• CAS Number: 9007-83-4
• Certification: RUO
• Host: Mouse
• Species Reactivity: Human
• Negative Species: Horse
• Immunogen: Human thymocytes and T lymphocytes.
• Target Antigen: CD45
• Isotype: IgG1
• Clone: MEM-28
• Applications: Flow cytometry: The reagent is designed for analysis of human blood cells using 20 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (2 mL) is sufficient for 100 tests.
• Validated Applications: FC
• Format: FITC
• Buffer: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• References & Citations: *Leukocyte Typing III., McMichael A. J. et al (Eds.), Oxford University Press (1987)., URL: ,*Horejsi V, Angelisova P, Bazil V, Kristofova H, Stoyanov S, Stefanova I, Hausner P, Vosecky M, Hilgert I: Monoclonal antibodies against human leucocyte antigens. II. Antibodies against CD45 (T200), CD3 (T3), CD43, CD10 (CALLA), transferrin receptor (T9), a novel broadly expressed 18-kDa antigen (MEM-43) and a novel antigen of restricted expression (MEM-74). Folia Biol (Praha). 198834(1):23-34., URL: https://www.ncbi.nlm.nih.gov/pubmed/2968928?dopt=A,*Ilangumaran S, Briol A, Hoessli DC: CD44 selectively associates with active Src family protein tyrosine kinases Lck and Fyn in glycosphingolipid-rich plasma membrane domains of human peripheral blood lymphocytes. Blood. 1998 May 1591(10):3901-8., URL: https://www.ncbi.nlm.nih.gov/pubmed/9573028?itool=,*Drbal K, Moertelmaier M, Holzhauser C, Muhammad A, Fuertbauer E, Howorka S, Hinterberger M, Stockinger H, Schütz GJ: Single-molecule microscopy reveals heterogeneous dynamics of lipid raft components upon TCR engagement. Int Immunol. 2007 May19(5):675-84., URL: https://www.ncbi.nlm.nih.gov/pubmed/17446208?itool,*Koethe S, Zander L, Köster S, Annan A, Ebenfelt A, Spencer J, Bemark M: Pivotal advance: CD45RB glycosylation is specifically regulated during human peripheral B cell differentiation. J Leukoc Biol. 2011 Jul90(1):5-19., URL: http://www.ncbi.nlm.nih.gov/pubmed?term=koethe%20s%20zander%20l ,*Cermák L, Símová S, Pintzas A, Horejsí V, Andera L: Molecular mechanisms involved in CD43-mediated apoptosis of TF-1 cells. Roles of transcription Daxx expression, and adhesion molecules. J Biol Chem. 2002 Mar 8277(10):7955-61., URL: http://www.ncbi.nlm.nih.gov/pubmed?term=%C4%8Derm%C3%A1k%20l%20%C5%A1%C3%ADmov%C3%A1%20%C5%A1%20pintzas%20a ,*McCormack E, Mujić M, Osdal T, Bruserud 216, Gjertsen BT: Multiplexed mAbs: a new strategy in preclinical time-domain imaging of acute myeloid leukemia. Blood. 2013 Feb 14121(7):e34-42. doi: 10.1182/blood-2012-05-429555., URL: http://www.ncbi.nlm.nih.gov/pubmed/?term=osdal+t+bruserud+o ,*Majer F, Vlaskova H, Krol L, Kalina T, Kubanek M, Stolnaya L, Dvorakova L, Elleder M, Sikora J: Danon disease: a focus on processing of the novel LAMP2 mutation and comments on the beneficial use of peripheral white blood cells in the diagnosis of LAMP2 deficiency. Gene. 2012 May 1498(2):183-95., URL: https://pubmed.ncbi.nlm.nih.gov/22365987/,
• Other References: *Li FJ, Tsuyama N, Ishikawa H, Obata M, Abroun S, Liu S, Otsuyama K, Zheng X, Ma Z, Maki Y, Kawano MM: A rapid translocation of CD45RO but not CD45RA to lipid rafts in IL-6-induced proliferation in myeloma. Blood. 2005 Apr 15105(8):3295-302. , URL: https://www.ncbi.nlm.nih.gov/pubmed/15626731?ordin,*Cosenza-Nashat MA, Kim MO, Zhao ML, Suh HS, Lee SC: CD45 isoform expression in microglia and inflammatory cells in HIV-1 encephalitis. Brain Pathol. 2006 Oct16(4):256-65., URL: http://www.ncbi.nlm.nih.gov/pubmed/17107594?ordina,*Dawes R, Petrova S, Liu Z, Wraith D, Beverley PC, Tchilian EZ. Combinations of CD45 isoforms are crucial for immune function and disease. J Immunol. 2006 Mar 15176(6):3417-25., URL: http://www.ncbi.nlm.nih.gov/pubmed/16517710?ordina,*Bijian K, Zhang L, Shen SH: Collagen-mediated survival signaling is modulated by CD45 in Jurkat T cells. Mol Immunol. 2007 Jul44(15):3682-90., URL: http://www.ncbi.nlm.nih.gov/pubmed/17524482?ordina,*Desharnais P, Dupéré-Minier G, Hamelin C, Devine P, Bernier J: Involvement of CD45 in DNA fragmentation in apoptosis induced by mitochondrial perturbing agents. Apoptosis. 2007 Dec 19. , URL: http://www.ncbi.nlm.nih.gov/pubmed/18157742?ordina,
• Storage Conditions: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.