Anti-Cytokeratin 8 FITC

• Description:

  Cytokeratins are a subfamily of intermediate filaments and are characterized by remarkable biochemical diversity. They are represented in epithelial tissues by at least 20 different polypeptides, molecular weight between 40 kDa and 68 kDa. The individual cytokeratin polypeptides are designated 1 to 20 and divided into the type I (acidic cytokeratins 9-20) and type II (basic to neutral cytokeratins 1-8) families.

• Certification:

  RUO

• Negative Species:

  Mouse, Rat, Xenopus, Hamster, Chicken

• Reactivity:

  Cow, Human, Rabbit, Pig, Sheep

• Immunogen:

  Cytoskeleton preparation from HeLa human cervix carcinoma cell line.

• Target Antigen:

  Cytokeratin 8

• Clone:

  C-43

• Applications:

  FC-IC (QC tested)

• Concentration:

  1 mg/mL

• Format:

  FITC

• Buffer:

  Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

• References & Citations:

  *Kovarik J et al.: J Tumor Marker Oncol. 5, 219 (1990) ., URL: , *Bartek J, Vojtesek B, Staskova Z, Bartkova J, Kerekes Z, Rejthar A, Kovarik J: A series of 14 new monoclonal antibodies to keratins: characterization and value in diagnostic histopathology. J Pathol. 1991 Jul;164 (3) :215-24. , URL: https://www.ncbi.nlm.nih.gov/pubmed/1716305?dopt=A, *Sikorski K, Mehta A, Inngjerdingen M, Thakor F, Kling S, Kalina T, Nyman TA, Stensland ME, Zhou W, de Souza GA, Holden L, Stuchly J, Templin M, Lund-Johansen F: A high-throughput pipeline for validation of antibodies. Nat Methods. 2018 Nov;15 (11) :909-912., URL: https://www.ncbi.nlm.nih.gov/pubmed/30377371

• Storage Conditions:

  Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

• Specificity:

  The antibody C-43 reacts with cytokeratin 8 (52.5 kDa intracellular antigen) . Cytokeratins are members of intermediate filaments subfamily represented in epithelial tissues.

• Applications Notes:

  Flow cytometry: Recommended dilution: 1-2 μg/ml. Intracellular staining.

• Isotype:

  Mouse IgG1