Anti-Hu CD81 Biotin

Anti-Hu CD81 Biotin

• Background: CD81 (TAPA-1), a member of the tetraspanin family, is expressed on virtually all nucleated cells, but above all on germinal center B cells. CD81 forms complexes with other tetraspanin proteins, integrins, coreceptors, MHC class I and II molecules, and influences adhesion, morphology, activation, proliferation and differentiation of B, T and other cells, e.g. in muscles CD81 promotes cell fusion and myotube maintenance. CD81 has been also identified as a receptor for the hepatitis C virus.
• Specifications: The antibody M38 reacts with an extracellular epitope of CD81, a 25 kDa member of the tetraspanin family, expressed on majority of cells.
• Certification: RUO
• Host: Mouse
• Species Reactivity: Rabbit, Cat, Human
• Immunogen: MOLT-4 (human T-ALL cell line)
• Target Antigen: CD81
• Isotype: IgG1
• Clone: M38
• Applications: Flow cytometry: recommended dilution: 1 μg/mL.
• Validated Applications: FC, IP, WB, IHC-P, ICC
• Concentration: 1 mg/mL
• Format: Biotin
• Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• References & Citations: *Imai T, Yoshie O: C33 antigen and M38 antigen recognized by monoclonal antibodies inhibitory to syncytium formation by human T cell leukemia virus type 1 are both members of the transmembrane 4 superfamily and associate with each other and with CD4 or CD8 in T cells. J Immunol. 1993 Dec 1151(11):6470-81., URL: https://www.ncbi.nlm.nih.gov/pubmed/8245480?ordina,*Imai T, Kakizaki M, Nishimura M, Yoshie O: Molecular analyses of the association of CD4 with two members of the transmembrane 4 superfamily, CD81 and CD82. J Immunol. 1995 Aug 1155(3):1229-39. , URL: https://www.ncbi.nlm.nih.gov/pubmed/7636191?ordina,*Escola JM, Kleijmeer MJ, Stoorvogel W, Griffith JM, Yoshie O, Geuze HJ: Selective enrichment of tetraspan proteins on the internal vesicles of multivesicular endosomes and on exosomes secreted by human B-lymphocytes. J Biol Chem. 1998 Aug 7273(32):20121-7., URL: https://www.ncbi.nlm.nih.gov/pubmed/9685355?ordina,*Fukudome K, Furuse M, Imai T, Nishimura M, Takagi S, Hinuma Y, Yoshie O: Identification of membrane antigen C33 recognized by monoclonal antibodies inhibitory to human T-cell leukemia virus type 1 (HTLV-1)-induced syncytium formation: altered glycosylation of C33 antigen in HTLV-1-positive T cells. J Virol. 1992 Mar66(3):1394-401., URL: http://www.ncbi.nlm.nih.gov/pubmed/1738199?ordinal,*Stehlíková O, Chovancová J, Tichý B, Krejčí M, Brychtová Y, Panovská A, Francová Skuhrová H, Burčková K, Borský M, Loja T, Mayer J, Pospíšilová S, Doubek M: Detecting minimal residual disease in patients with chronic lymphocytic leukemia using 8-color flow cytometry protocol in routine hematological practice. Int J Lab Hematol. 2013 Sep 13. doi: 10.1111/ijlh.12149., URL: http://www.ncbi.nlm.nih.gov/pubmed/24028768 ,*Fritzsching B, Schwer B, Kartenbeck J, Pedal A, Horejsi V, Ott M: Release and intercellular transfer of cell surface CD81 via microparticles. J Immunol. 2002 Nov 15169(10):5531-7., URL: https://pubmed.ncbi.nlm.nih.gov/12421929/,
• Storage Conditions: Store at 2-8°C. Do not freeze.