Anti-Hu CD105 Biotin

Anti-Hu CD105 Biotin

• Background: CD105 (endoglin) is a homodimeric transmembrane glycoprotein serving in presence of TGFbetaR-2 as a receptor for TGFbeta-1 and TGFbeta-3. CD105 is highly expressed on endothelial cells and promotes angiogenesis during wound healing, infarcts and in a wide range of tumours and its gene expression is stimulated by hypoxia. CD105 prevents apoptosis in hypoxic endothelial cells and also antagonises the inhibitory effects of TGFbeta-1 on vascular endothelial cell growth and migration. Normal cellular levels of CD105 are required for formation of new blood vessels.
• Specifications: The antibody MEM-229 recognizes an extracellular epitope of CD105 (Endoglin), a 90 kDa type I integral membrane homodimer glycoprotein expressed on vascular endothelial cells (small and large vessels), activated monocytes and tissue macrophages, stromal cells of certain tissues including bone marrow, pre-B lymphocytes in fetal marrow and erythroid precursors in fetal and adult bone marrow it is also present on syncytiotrophoblast on placenta throughout pregnancy.
• CAS Number: 9007-83-4
• Certification: RUO
• Host: Mouse
• Species Reactivity: Pig, Human
• Negative Species: Dog, Horse
• Immunogen: Recombinant Vaccinia virus containing the human CD105 (L-isoform) cDNA.
• Target Antigen: CD105
• Isotype: IgG2a
• Clone: MEM-229
• Applications: Flow cytometry: Recommended dilution: 8 μg/mL positive control: Kg1 human acute myelogenous leukemia cell line.
• Validated Applications: FC, WB, IHC-F, ICC
• Concentration: 1 mg/mL
• Format: Biotin
• Buffer: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• References & Citations: *Plánka L, Necas A, Srnec R, Rauser P, Starý D, Jancár J, Amler E, Filová E, Hlucilová J, Kren L, Gál P: Use of allogenic stem cells for the prevention of bone bridge formation in miniature pigs. Physiol Res. 200958(6):885-93., URL: https://www.ncbi.nlm.nih.gov/pubmed/19093735,
• Other References: *Zhu Y, Sun Y, Xie L, Jin K, Sheibani N, Greenberg DA: Hypoxic induction of endoglin via mitogen-activated protein kinases in mouse brain microvascular endothelial cells. Stroke. 2003 Oct34(10):2483-8., URL: https://www.ncbi.nlm.nih.gov/pubmed/12947156?ordin,*Li C, Issa R, Kumar P, Hampson IN, Lopez-Novoa JM, Bernabeu C, Kumar S: CD105 prevents apoptosis in hypoxic endothelial cells. J Cell Sci. 2003 Jul 1116(Pt 13):2677-85., URL: https://www.ncbi.nlm.nih.gov/pubmed/12746487?ordin,*Guo B, Slevin M, Li C, Parameshwar S, Liu D, Kumar P, Bernabeu C, Kumar S: CD105 inhibits transforming growth factor-beta-Smad3 signalling. Anticancer Res. 2004 May-Jun24(3a):1337-45., URL: https://www.ncbi.nlm.nih.gov/pubmed/15274293?ordin,*Warrington K, Hillarby MC, Li C, Letarte M, Kumar S: Functional role of CD105 in TGF-beta1 signalling in murine and human endothelial cells. Anticancer Res. 2005 May-Jun25(3B):1851-64., URL: https://www.ncbi.nlm.nih.gov/pubmed/16158917?ordin,*Piao M, Tokunaga O: Significant expression of endoglin (CD105), TGFbeta-1 and TGFbeta R-2 in the atherosclerotic aorta: an immunohistological study. J Atheroscler Thromb. 2006 Apr13(2):82-9., URL: https://www.ncbi.nlm.nih.gov/pubmed/16733295?ordin,
• Storage Conditions: Store at 2-8°C. Do not freeze.