Anti-Hu CD15 Biotin

Anti-Hu CD15 Biotin

• Background: CD15 (Lewis x), also known as stage specific embryonic antigen-1 (SSEA-1) is a trisacharide determinant (3-fucosyl-N-acetyllactosamine) expressed on several glycolipids, glycoproteins and proteoglycans of various cell types, e.g. granulocytes, mast cells, monocytes, macrophages, cells of gastric mucosa, nervous system or various tumour cells. There are several structural relatives of Lewis x, e.g. sialyl-Lewis x or sulphated Lewis x. Cells with high surface expression of Le(x) antigen exhibit strong self-aggregation, based on calcium-dependent Le(x)-Le(x) interaction. This process is involved for example in embryo compaction or in autoaggregation of teratocarcinoma cells. Sialyl-Le(x) and its isomer sialyl-Le(a) are ligands of selectins. CD15 expression has been extensively used to confirm diagnosis of Hodgkin´s disease.
• Specifications: The antibody MEM-158 reacts with CD15, a cell membrane molecule 3-fucosyl-N-acetyllactosamine (3-FAL) strongly expressed on the surface of granulocytes, monocytes, macrophages, mast cells it is also present on Langerhans cells and some myeloid precursors cells.
• CAS Number: 9007-83-4
• Certification: RUO
• Host: Mouse
• Species Reactivity: Human
• Negative Species: Pig, Sheep, Cow
• Immunogen: Human granulocytes
• Target Antigen: CD15
• Isotype: IgM
• Clone: MEM-158
• Applications: Flow cytometry: recommended dilution: 1-4 μg/mL.
• Validated Applications: FC, IP
• Concentration: 1 mg/mL
• Format: Biotin
• Buffer: Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide
• References & Citations: *Sutherland DR, Richards SJ, Ortiz F, Nayyar R, Benko M, Marinov I, Illingworth A: CD71 improves delineation of PNH type III, PNH type II, and normal immature RBCS in patients with paroxysmal nocturnal hemoglobinuria. Cytometry B Clin Cytom. 2019 Nov 8. doi: 10.1002/cyto.b.21853. , URL: https://www.ncbi.nlm.nih.gov/pubmed/31705743,*Sutherland DR, Illingworth A, Marinov I, Ortiz F, Andreasen J, Payne D, Wallace PK, Keeney M: ICCS/ESCCA consensus guidelines to detect GPI-deficient cells in paroxysmal nocturnal hemoglobinuria (PNH) and related disorders part 2 - reagent selection and assay optimization for high-sensitivity testing. Cytometry B Clin Cytom. 2018 Jan94(1):23-48., URL: https://www.ncbi.nlm.nih.gov/pubmed/29236353,*Sutherland DR, Ortiz F, Quest G, Illingworth A, Benko M, Nayyar R, Marinov I: High-sensitivity 5-, 6-, and 7-color PNH WBC assays for both Canto II and Navios platforms. Cytometry B Clin Cytom. 2018 Jul94(4):637-651., URL: https://www.ncbi.nlm.nih.gov/pubmed/29381839 ,
• Other References: *Benharroch D, Dima E, Levy A, Ohana-Malka O, Ariad S, Prinsloo I, Mejirovsky E, Sacks M, Gopas J: Differential expression of sialyl and non-sialyl-CD15 antigens on Hodgkin-Reed-Sternberg cells: significance in Hodgkin's disease. Leuk Lymphoma. 2000 Sep39(1-2):185-94., URL: https://pubmed.ncbi.nlm.nih.gov/10975398/,*Hakomori S: Le(X) and related structures as adhesion molecules. Histochem J. 1992 Nov24(11):771-6., URL: https://pubmed.ncbi.nlm.nih.gov/1362193/,*Li C, Wong P, Pan T, Xiao F, Yin S, Chang B, Kang SC, Ironside J, Sy MS: Normal cellular prion protein is a ligand of selectins: binding requires Le(X) but is inhibited by sLe(X). Biochem J. 2007 Sep 1406(2):333-41., URL: https://pubmed.ncbi.nlm.nih.gov/17497959/,
• Storage Conditions: Store at 2-8°C. Do not freeze.