Anti-Hu CD1c APC

CD1c (also known as R7 or BDCA1) together with CD1a and b, belongs to group 1 of CD1 antigens. These non-classical MHC-like glycoproteins serve as antigen-presenting molecules for a subset of T cells that responds to specific lipids and glycolipids found in the cell walls of bacterial pathogens or self-glycolipid antigens such as gangliosides, and they have also roles in antiviral immunity. The trafficking routes of the particular CD1 types differ and correspond to their ability to bind and present different groups of antigens. CD1c is unique in its ability to present e.g. mycobacterial phosphoketides and polyisoprenoids. CD1c is the only CD1 isoform that has been shown to interact both with alpha/beta and gamma/delta T cells.

Human

Human thymocytes

CD1c

L161

FC (QC tested)

APC

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

*del C Salamone M, Mendiguren AK, Salamone GV, Fainboim L: Membrane trafficking of CD1c on activated T cells. J Leukoc Biol. 2001 Oct;70 (4) :567-77., URL: https://pubmed.ncbi.nlm.nih.gov/11590193/, *Xu C, de Vries R, Visser L, Diepstra A, Gadola SD, Poppema S, van den Berg A: Expression of CD1d and presence of invariant NKT cells in classical Hodgkin lymphoma. Am J Hematol. 2010 Jul;85 (7) :539-41. doi: 10.1002/ajh.21743., URL: https://www.ncbi.nlm.nih.gov/pubmed/20575029 , *Todate A, Chida K, Suda T, Imokawa S, Sato J, Ide K, Tsuchiya T, Inui N, Nakamura Y, Asada K, Hayakawa H, Nakamura H: Increased numbers of dendritic cells in the bronchiolar tissues of diffuse panbronchiolitis. Am J Respir Crit Care Med. 2000 Jul;162 (1) :148-53., URL: http://www.ncbi.nlm.nih.gov/pubmed/10903234 , *Scharf L, Li NS, Hawk AJ, Garzón D, Zhang T, Fox LM, Kazen AR, Shah S, Haddadian EJ, Gumperz JE, Saghatelian A, Faraldo-Gómez JD, Meredith SC, Piccirilli JA, Adams EJ: The 2.5 Å structure of CD1c in complex with a mycobacterial lipid reveals an open groove ideally suited for diverse antigen presentation. Immunity. 2010 Dec 14;33 (6) :853-62., URL: https://pubmed.ncbi.nlm.nih.gov/21167756/, *Gutzeit C, Raftery MJ, Peiser M, Tischer KB, Ulrich M, Eberhardt M, Stockfleth E, Giese T, Sauerbrei A, Morita CT, Schönrich G: Identification of an important immunological difference between virulent varicella-zoster virus and its avirulent vaccine: viral disruption of dendritic cell instruction. J Immunol. 2010 Jul 1;185 (1) :488-97., URL: https://pubmed.ncbi.nlm.nih.gov/20525895/, *Briken V, Jackman RM, Watts GF, Rogers RA, Porcelli SA: Human CD1b and CD1c isoforms survey different intracellular compartments for the presentation of microbial lipid antigens. J Exp Med. 2000 Jul 17;192 (2) :281-8., URL: https://www.ncbi.nlm.nih.gov/pubmed/10899914, *del C Salamone M, Mendiguren AK, Salamone GV, Fainboim L: Membrane trafficking of CD1c on activated T cells. J Leukoc Biol. 2001 Oct;70 (4) :567-77., URL: https://pubmed.ncbi.nlm.nih.gov/11590193/, *Quardokus EM, Saunders DC, McDonough E, Hickey JW, Werlein C, Surrette C, Rajbhandari P, Martinez Casals A, Tian H, Lowery L, Neumann EK, Björklund F, Neelakantan TV, Croteau J, Wiblin AE, Fisher J, Livengood AJ, Dowell KG, Silverstein JC, Spraggins JM, Pryhuber GS, Deutsch G, Ginty F, Nolan GP, Melov S, Jonigk D, Caldwell MA, Vlachos IS, Mueller W, Gehlenborg N, Stockwell BR, Lundberg E, Snyder MP, Germain RN, Camarillo JM, Kelleher NL, Börner K, Radtke AJ: Organ mapping antibody panels: a community resource for standardized multiplexed tissue imaging. Nat Methods 2023 Aug;20 (8) :1174-1178. doi: 10.1038/s41592-023-01846-7., URL: https://pubmed.ncbi.nlm.nih.gov/37468619/

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

The mouse monoclonal antibody L161 recognizes an extracellular epitope of CD1c, (R7), a 43 kDa type I glycoprotein associated with beta2-microglobulin. It is expressed on cortical thymocytes (strongly), Langerhans cells, dendritic cells, B and some T cells.

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