Anti-Hu CD94 APC

Anti-Hu CD94 APC

• Background: CD94, also known as KLRD1 (killer cell lectin-like receptor D1), is a transmembrane glycoprotein of the C-type lectin family, which forms disulfide-linked heterodimers with NKG2A, B, C, E, H proteins, constituting functionally distinct receptors of NK cells and related cell types. CD94/NKG2A and CD94/NKG2B heterodimers serve as inhibitory, whereas CD94/NKG2C and CD94/NKG2E as activating receptors. The ligand for CD94/NKG2 complexes has been identified as HLA-E. Extent of CD94 expression on NK cell surface can be used to demonstrate their progress through the differentiation process.
• Specifications: The mouse monoclonal antibody HP-3D9 recognizes an extracellular epitope of CD94, a 70 kDa type II transmembrane glycoprotein expressed on NK cells, NK-T cells, and subsets of CD8+ T cells and gamma/delta T cells.
• CAS Number: 9007-83-4
• Certification: RUO
• Host: Mouse
• Species Reactivity: Human
• Immunogen: Cultured human NK cells
• Target Antigen: CD94
• Isotype: Igg1 κ
• Clone: HP-3D9
• Applications: Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (1 mL) is sufficient for 100 tests.
• Validated Applications: FC
• Format: APC
• Buffer: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• References & Citations: *Yu J, Mao HC, Wei M, Hughes T, Zhang J, Park IK, Liu S, McClory S, Marcucci G, Trotta R, Caligiuri MA: CD94 surface density identifies a functional intermediary between the CD56bright and CD56dim human NK-cell subsets. Blood. 2010 Jan 14115(2):274-81, URL: http://www.ncbi.nlm.nih.gov/pubmed/19897577 ,*Phillips JH, Chang C, Mattson J, Gumperz JE, Parham P, Lanier LL: CD94 and a novel associated protein (94AP) form a NK cell receptor involved in the recognition of HLA-A, HLA-B, and HLA-C allotypes. Immunity. 1996 Aug5(2):163-72., URL: http://www.ncbi.nlm.nih.gov/pubmed/8769479 ,*Seo N, Tokura Y, Ishihara S, Takeoka Y, Tagawa S, Takigawa M: Disordered expression of inhibitory receptors on the NK1-type natural killer (NK) leukaemic cells from patients with hypersensitivity to mosquito bites. Clin Exp Immunol. 2000 Jun120(3):413-9., URL: http://www.ncbi.nlm.nih.gov/pubmed/10844517 ,*Hallermalm K, Seki K, De Geer A, Motyka B, Bleackley RC, Jager MJ, Froelich CJ, Kiessling R, Levitsky V, Levitskaya J: Modulation of the tumor cell phenotype by IFN-gamma results in resistance of uveal melanoma cells to granule-mediated lysis by cytotoxic lymphocytes. J Immunol. 2008 Mar 15180(6):3766-74., URL: http://www.ncbi.nlm.nih.gov/pubmed/18322182 ,*Bovenschen HJ, Van De Kerkhof PC, Gerritsen WJ, Seyger MM: The role of lesional T cells in recalcitrant psoriasis during infliximab therapy. Eur J Dermatol. 2005 Nov-Dec15(6):454-8., URL: http://www.ncbi.nlm.nih.gov/pubmed/16280298 ,*Ntrivalas EI, Kwak-Kim JY, Gilman-Sachs A, Chung-Bang H, Ng SC, Beaman KD, Mantouvalos HP, Beer AE: Status of peripheral blood natural killer cells in women with recurrent spontaneous abortions and infertility of unknown aetiology. Hum Reprod. 2001 May16(5):855-61., URL: http://www.ncbi.nlm.nih.gov/pubmed/11331628 ,*Romero P, Ortega C, Palma A, Molina IJ, Pe241a J, Santamaría M: Expression of CD94 and NKG2 molecules on human CD4(+) T cells in response to CD3-mediated stimulation. J Leukoc Biol. 2001 Aug70(2):219-24., URL: http://www.ncbi.nlm.nih.gov/pubmed/11493613,*Wada H, Matsumoto N, Maenaka K, Suzuki K, Yamamoto K: The inhibitory NK cell receptor CD94/NKG2A and the activating receptor CD94/NKG2C bind the top of HLA-E through mostly shared but partly distinct sets of HLA-E residues. Eur J Immunol. 2004 Jan34(1):81-90., URL: http://www.ncbi.nlm.nih.gov/pubmed/14971033 ,
• Storage Conditions: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.