Anti-Hu CD150 APC

• Description:

  CD150, also known as SLAM (signaling lymphocyte activation molecule) is a 70-95 kDa single chain transmembrane phosphoglycoprotein of the CD2 family. Its extracellular part contains eight potential N-glycosylation sites, and the intracellular tail contains three unique tyrosine-based motifs. These binding sites can be recognized by SH2-binding phosphatases and the adaptor proteins, such as SAP/SH2D1A or EAT-2. The SLAM family receptors are involved in leucocyte activation and contribute to the effective germinal center formation, generation of high-affinity antibody-secreting plasma cells, and memory T and B cells, thereby facilitating long-term immune response. CD150 expression is upregulated after cell activation.

• Certification:

  RUO

• Reactivity:

  Human

• Immunogen:

  Human CD150-transfected 300.19 cells

• Target Antigen:

  CD150

• Clone:

  SLAM.4

• Applications:

  FC (QC tested)

• Format:

  APC

• Buffer:

  Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

• References & Citations:

  *Romero X, Zapater N, Calvo M, Kalko SG, de la Fuente MA, Tovar V, Ockeloen C, Pizcueta P, Engel P: CD229 (Ly9) lymphocyte cell surface receptor interacts homophilically through its N-terminal domain and relocalizes to the immunological synapse. J Immunol. 2005 Jun 1;174 (11) :7033-42., URL: http://www.ncbi.nlm.nih.gov/pubmed/15905546 De Sal, *Romero X, Benítez D, March S, Vilella R, Miralpeix M, Engel P: Differential expression of SAP and EAT-2-binding leukocyte cell-surface molecules CD84, CD150 (SLAM), CD229 (Ly9) and CD244 (2B4) . Tissue Antigens. 2004 Aug;64 (2) :132-44., URL: http://www.ncbi.nlm.nih.gov/pubmed/15245368, *De Salort J, Sintes J, Llinàs L, Matesanz-Isabel J, Engel P: Expression of SLAM (CD150) cell-surface receptors on human B-cell subsets: from pro-B to plasma cells. Immunol Lett. 2011 Jan 30;134 (2) :129-36., URL: https://www.ncbi.nlm.nih.gov/pubmed/20933013, *Romero X, Martin M, Zapater N, Tovar V, Pizcueta P, Engel P: Expression of CD150 on human leukocytes. Production and characterisation of a new CD150 monoclonal antibody, URL:

• Other References:

  *García VE, Quiroga MF, Ochoa MT, Ochoa L, Pasquinelli V, Fainboim L, Olivares LM, Valdez R, Sordelli DO, Aversa G, Modlin RL, Sieling PA: Signaling lymphocytic activation molecule expression and regulation in human intracellular infection correlate with Th1 cytokine patterns. J Immunol. 2001 Nov 15;167 (10) :5719-24., URL: http://www.ncbi.nlm.nih.gov/pubmed/11698444

• Storage Conditions:

  Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

• Specificity:

  The mouse monoclonal antibody SLAM.4 recognizes an extracellular epitope of CD150, a cell surface molecule expressed on lymphocytes and involved in their activation.

• Applications Notes:

  6

• Isotype:

  Mouse IgG1