Anti-Ms CD25 APC

CD25 (IL2Ralpha, Tac) is a ligand-binding alpha subunit of interleukin 2 receptor (IL2R) . Together with beta and gamma subunit CD25 constitues the high affinity IL2R, whereas CD25 alone serves as the low affinity IL2R. CD25 expression rapidly increases upon T cell activation. The 55 kDa CD25 molecule is enzymatically cleaved and shed from the cell surface as a soluble 45 kDa s-Tac, whose concentration in serum can be used as a marker of T cell activation. Expression of CD25 indicates the neoplastic phenotype of mast cells. Humanized anti CD25 antibodies represent a useful tool to reduce the incidence of allograft rejection as well as the severity of graft versus host reaction, and radioimmunoconjugates of anti-CD25 antibodies can be used against CD25 expressing lymphomas.

Mouse

B6.1 CTL cell line

CD25

PC61.5

FC (QC tested)

0.5 mg/mL

APC

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

*Tenorio EP, Olguín JE, Fernández J, Vieyra P, Saavedra R: Reduction of Foxp3+ cells by depletion with the PC61 mAb induces mortality in resistant BALB/c mice infected with Toxoplasma gondii. J Biomed Biotechnol. 2010;2010:786078., URL: http://www.ncbi.nlm.nih.gov/pubmed/20037737 , *Wei HX, Chuang YH, Li B, Wei H, Sun R, Moritoki Y, Gershwin ME, Lian ZX, Tian Z: CD4+ CD25+ Foxp3+ regulatory T cells protect against T cell-mediated fulminant hepatitis in a TGF-beta-dependent manner in mice. J Immunol. 2008 Nov 15;181 (10) :7221-9., URL: http://www.ncbi.nlm.nih.gov/pubmed/18981144 , *Yu CT, Feng MH, Shih HM, Lai MZ: Increased p300 expression inhibits glucocorticoid receptor-T-cell receptor antagonism but does not affect thymocyte positive selection. Mol Cell Biol. 2002 Jul;22 (13) :4556-66., URL: https://www.ncbi.nlm.nih.gov/pubmed/12052865, *Aiba Y, Hirayama F, Ogawa M: Clonal proliferation and cytokine requirement of murine progenitors for natural killer cells. Blood. 1997 Jun 1;89 (11) :4005-12., URL: https://www.ncbi.nlm.nih.gov/pubmed/9166839, *Kish DD, Gorbachev AV, Fairchild RL: CD8+ T cells produce IL-2, which is required for CD (4+) CD25+ T cell regulation of effector CD8+ T cell development for contact hypersensitivity responses. J Leukoc Biol. 2005 Sep;78 (3) :725-35., URL: https://www.ncbi.nlm.nih.gov/pubmed/16000396, *Van VQ, Darwiche J, Raymond M, Lesage S, Bouguermouh S, Rubio M, Sarfati M: Cutting edge: CD47 controls the in vivo proliferation and homeostasis of peripheral CD4+ CD25+ Foxp3+ regulatory T cells that express CD103. J Immunol. 2008 Oct 15;181 (8) :5204-8., URL: https://www.ncbi.nlm.nih.gov/pubmed/18832672, *Kotner J, Tarleton R: Endogenous CD4 (+) CD25 (+) regulatory T cells have a limited role in the control of Trypanosoma cruzi infection in mice. Infect Immun. 2007 Feb;75 (2) :861-9., URL: https://www.ncbi.nlm.nih.gov/pubmed/17101658, *Jones CM, Cose SC, Coles RM, Winterhalter AC, Brooks AG, Heath WR, Carbone FR: Herpes simplex virus type 1-specific cytotoxic T-lymphocyte arming occurs within lymph nodes draining the site of cutaneous infection. J Virol. 2000 Mar;74 (5) :2414-9., URL: https://www.ncbi.nlm.nih.gov/pubmed/10666272

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

+

Flow cytometry: Recommended dilution: 1-2 μg/ml.