Anti-Ms CD8a APC

Anti-Ms CD8a APC

• Background: The CD8a (CD8 alpha) subunit of CD8 T cell coreceptor is expressed in CD8 alpha/beta heterodimers on majority of MHC I-restricted conventional T cells and thymocytes and in CD8 alpha/alpha homodimers on subsets of memory T cells, intraepithelial lymphocytes, NK cells, macrophages and dendritic cells. Regulation of CD8 beta level on T cell surface seems to be an important mechanism to control their effector function. Assembly of CD8 alpha/beta but not alpha/alpha dimers is connected with formation or localization to the lipid rafts. Recruiting triggered TCR complexes to these membrane microdomains as well as affinity of TCR to MHC I is modulated by CD8, thereby affecting the functional diversity of the TCR signaling.
• Specifications: The rat monoclonal antibody 53-6.7 recognizes an extracellular epitope of murine CD8a (32-34 kDa alpha chain of the CD8 antigen).
• CAS Number: 9007-83-4
• Certification: RUO
• Host: Rat
• Species Reactivity: Mouse
• Immunogen: Murine spleen cells
• Target Antigen: CD8a
• Isotype: IgG2a κ
• Clone: 53-6.7
• Applications: Flow cytometry: Recommended dilution: 1-2 μg/mL.
• Validated Applications: FC
• Concentration: 0.5 mg/mL
• Format: APC
• Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• References & Citations: *Mochimaru H, Usui T, Yaguchi T, Nagahama Y, Hasegawa G, Usui Y, Shimmura S, Tsubota K, Amano S, Kawakami Y, Ishida S: Suppression of alkali burn-induced corneal neovascularization by dendritic cell vaccination targeting VEGF receptor 2. Invest Ophthalmol Vis Sci. 2008 May49(5):2172-7., URL: https://www.ncbi.nlm.nih.gov/pubmed/18263815?ordin,*Bouwer HG, Alberti-Segui C, Montfort MJ, Berkowitz ND, Higgins DE: Directed antigen delivery as a vaccine strategy for an intracellular bacterial pathogen. Proc Natl Acad Sci U S A. 2006 Mar 28103(13):5102-7., URL: https://www.ncbi.nlm.nih.gov/pubmed/16549792?ordin,*Kamimura D, Sawa Y, Sato M, Agung E, Hirano T, Murakami M: IL-2 in vivo activities and antitumor efficacy enhanced by an anti-IL-2 mAb. J Immunol. 2006 Jul 1177(1):306-14., URL: https://www.ncbi.nlm.nih.gov/pubmed/16785526?ordin,*Kosy, Ko HJ, Chang WS, Park SH, Kweon MN, Kang CY: alpha-Galactosylceramide can act as a nasal vaccine adjuvant inducing protective immune responses against viral infection and tumor. J Immunol. 2005 Sep 1175(5):3309-17., URL: https://www.ncbi.nlm.nih.gov/pubmed/16116223?ordin,*Grabbe S, Varga G, Beissert S, Steinert M, Pendl G, Seeliger S, Bloch W, Peters T, Schwarz T, Sunderkötter C, Scharffetter-Kochanek K: Beta2 integrins are required for skin homing of primed T cells but not for priming naive T cells. J Clin Invest. 2002 Jan109(2):183-92., URL: https://www.ncbi.nlm.nih.gov/pubmed/11805130?ordin,*Hata H, Sakaguchi N, Yoshitomi H, Iwakura Y, Sekikawa K, Azuma Y, Kanai C, Moriizumi E, Nomura T, Nakamura T, Sakaguchi S. Distinct contribution of IL-6, TNF-alpha, IL-1, and IL-10 to T cell-mediated spontaneous autoimmune arthritis in mice. J Clin Invest. 2004 Aug114(4):582-8., URL: https://www.ncbi.nlm.nih.gov/pubmed/15314695?ordin,*Takahashi K, Nakata M, Tanaka T, Adachi H, Nakauchi H, Yagita H, Okumura K: CD4 and CD8 regulate interleukin 2 responses of T cells. Proc Natl Acad Sci U S A. 1992 Jun 1589(12):5557-61., URL: https://www.ncbi.nlm.nih.gov/pubmed/1608966?ordina,*Ledbetter JA, Rouse RV, Micklem HS, Herzenberg LA: T cell subsets defined by expression of Lyt-1,2,3 and Thy-1 antigens. Two-parameter immunofluorescence and cytotoxicity analysis with monoclonal antibodies modifies current views. J Exp Med. 1980 Aug 1152(2):280-95., URL: https://www.ncbi.nlm.nih.gov/pubmed/6156984?ordina,*Ledbetter JA, Herzenberg LA: Xenogeneic monoclonal antibodies to mouse lymphoid differentiation antigens. Immunol Rev. 197947:63-90., URL: https://www.ncbi.nlm.nih.gov/pubmed/398327?ordinal,
• Storage Conditions: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.