Anti-Ms CD9 APC

CD9 belongs to proteins of tetraspanin family that orchestrate cholesterol-associated tetraspanin-enriched signaling microdomains within the plasma membrane, forming complexes with each other as well as with integrins, membrane-anchored growth factors and other proteins. CD9 is involved in cell motility, osteoclastogenesis, neurite outgrowth, myotube formation, and sperm-egg fusion, plays roles in cell attachment and proliferation and is necessary for association of heterologous MHC II molecules on the dendritic cell plasma membrane which is important for effective T cell stimulation. CD9 is also considered as metastasis suppressor in solid tumors.

Mouse

Permeabilized murine bone marrow-derived mast cells (BMMC) .

CD9

EM-04

FC (QC tested)

0.5 mg/mL

APC

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

*Athman JJ, Wang Y, McDonald DJ, Boom WH, Harding CV, Wearsch PA: Bacterial membrane vesicles mediate the release of Mycobacterium tuberculosis lipoglycans and lipoproteins from infected macrophages. J Immunol. 2015 Aug 1;195 (3) :1044-53., URL: https://www.ncbi.nlm.nih.gov/pubmed/?term=athman+jj+wang+y+boom+wh

*Kotha J, Zhang C, Longhurst CM, Lu Y, Jacobs J, Cheng Y, Jennings LK: Functional relevance of tetraspanin CD9 in vascular smooth muscle cell injury phenotypes: a novel target for the prevention of neointimal hyperplasia. Atherosclerosis. 2009 Apr;203 (2) :377-86., URL: http://www.ncbi.nlm.nih.gov/pubmed/18799160?ordina, *Liu WM, Cao YJ, Yang YJ, Li J, Hu Z, Duan EK: Tetraspanin CD9 regulates invasion during mouse embryo implantation. J Mol Endocrinol. 2006 Feb;36 (1) :121-30., URL: https://www.ncbi.nlm.nih.gov/pubmed/16461932?ordin, *Saito Y, Tachibana I, Takeda Y, Yamane H, He P, Suzuki M, Minami S, Kijima T, Yoshida M, Kumagai T, Osaki T, Kawase I. Absence of CD9 enhances adhesion-dependent morphologic differentiation, survival, and matrix metalloproteinase-2 production in small cell lung cancer cells. Cancer Res. 2006 Oct 1;66 (19) :9557-65., URL: https://pubmed.ncbi.nlm.nih.gov/17018612/, *Israels SJ, McMillan-Ward EM: Platelet tetraspanin complexes and their association with lipid rafts. Thromb Haemost. 2007 Nov;98 (5) :1081-7., URL: https://pubmed.ncbi.nlm.nih.gov/18000614/, *Kim YJ, Yu JM, Joo HJ, Kim HK, Cho HH, Bae YC, Jung JS: Role of CD9 in proliferation and proangiogenic action of human adipose-derived mesenchymal stem cells. Pflugers Arch. 2007 Nov;455 (2) :283-96., URL: https://pubmed.ncbi.nlm.nih.gov/17668233/, *Unternaehrer JJ, Chow A, Pypaert M, Inaba K, Mellman I: The tetraspanin CD9 mediates lateral association of MHC class II molecules on the dendritic cell surface. Proc Natl Acad Sci U S A. 2007 Jan 2;104 (1) :234-9., URL: https://pubmed.ncbi.nlm.nih.gov/17190803/

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

The rat monoclonal antibody EM-04 recognizes an extracellular epitope of CD9 antigen, a 24 kDa transmembrane protein expressed on platelets, monocytes, pre-B lymphocytes, granulocytes and activated T lymphocytes.

Flow cytometry: Recommended dilution: 2-3 μg/ml.