Anti-Ms CD45 APC

• Description:

  CD45 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase ubiquitously expressed in all nucleated hematopoietic cells, comprising approximately 10% of all surface proteins in lymphocytes. CD45 glycoprotein is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases. CD45 protein exists as multiple isoforms as a result of alternative splicing; these isoforms differ in their extracellular domains, whereas they share identical transmembrane and cytoplasmic domains. These isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. Besides the role in immunoreceptor signaling, CD45 is important in promoting cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis.

• Certification:

  RUO

• Reactivity:

  Mouse

• Immunogen:

  Murine peripheral blood leukocytes

• Target Antigen:

  CD45

• Clone:

  EM-05

• Applications:

  FC (QC tested)

• Concentration:

  0.5 mg/mL

• Format:

  APC

• Buffer:

  Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

• References & Citations:

  *Shen KY, Song YC, Chen IH, Chong P, Liu SJ: Depletion of tumor-associated macrophages enhances the anti-tumor immunity induced by a Toll-like receptor agonist-conjugated peptide. Hum Vaccin Immunother. 2014;10 (11) :3241-50., URL: https://www.ncbi.nlm.nih.gov/pubmed/25483652

• Other References:

  *Townsend KP, Vendrame M, Ehrhart J, Faza B, Zeng J, Town T, Tan J: CD45 isoform RB as a molecular target to oppose lipopolysaccharide-induced microglial activation in mice. Neurosci Lett. 2004 May 13;362 (1) :26-30., URL: https://www.ncbi.nlm.nih.gov/pubmed/15147773?ordin, *Desharnais P, Dupéré-Minier G, Hamelin C, Devine P, Bernier J: Involvement of CD45 in DNA fragmentation in apoptosis induced by mitochondrial perturbing agents. Apoptosis. 2007 Dec 19., URL: https://www.ncbi.nlm.nih.gov/pubmed/18157742?ordin, *Byth KF, Conroy LA, Howlett S, Smith AJ, May J, Alexander DR, Holmes N: CD45-null transgenic mice reveal a positive regulatory role for CD45 in early thymocyte development, in the selection of CD4+CD8+ thymocytes, and B cell maturation. J Exp Med. 1996 Apr 1;183 (4) :1707-18., URL: https://www.ncbi.nlm.nih.gov/pubmed/8666928?ordina, *Bijian K, Zhang L, Shen SH: Collagen-mediated survival signaling is modulated by CD45 in Jurkat T cells. Mol Immunol. 2007 Jul;44 (15) :3682-90., URL: https://www.ncbi.nlm.nih.gov/pubmed/17524482?ordin, *Dawes R, Petrova S, Liu Z, Wraith D, Beverley PC, Tchilian EZ. Combinations of CD45 isoforms are crucial for immune function and disease. J Immunol. 2006 Mar 15;176 (6) :3417-25., URL: https://www.ncbi.nlm.nih.gov/pubmed/16517710?ordin, *Cosenza-Nashat MA, Kim MO, Zhao ML, Suh HS, Lee SC: CD45 isoform expression in microglia and inflammatory cells in HIV-1 encephalitis. Brain Pathol. 2006 Oct;16 (4) :256-65., URL: https://www.ncbi.nlm.nih.gov/pubmed/17107594?ordin, *Li FJ, Tsuyama N, Ishikawa H, Obata M, Abroun S, Liu S, Otsuyama K, Zheng X, Ma Z, Maki Y, Kawano MM: A rapid translocation of CD45RO but not CD45RA to lipid rafts in IL-6-induced proliferation in myeloma. Blood. 2005 Apr 15;105 (8) :3295-302., URL: https://www.ncbi.nlm.nih.gov/pubmed/15626731?ordin

• Storage Conditions:

  Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

• Specificity:

  The rat monoclonal antibody EM-05 reacts with an extracellular epitope of murine CD45 antigen (Leukocyte Common Antigen), a single chain type I transmembrane protein expressed at high level on cells of hematopoietic origin, except erythrocytes and platelets.

• Applications Notes:

  Flow cytometry: Recommended dilution: 1-2 μg/ml; positive control: murine peripheral blood leukocytes.

• Isotype:

  Rat IgG