Anti-Hu CD5 APC

CD5 antigen (T1; 67 kDa) is a human cell surface T-lymphocyte single-chain transmembrane glycoprotein. CD5 is expressed on all mature T-lymphocytes, most of thymocytes, subset of B-lymphocytes and on many T-cell leukemias and lymphomas. It is a type I membrane glycoprotein whose extracellular region contains three scavenger receptor cysteine-rich (SRCR) domains. The CD5 is a signal transducing molecule whose cytoplasmic tail is devoid of any intrinsic catalytic activity. CD5 modulates signaling through the antigen-specific receptor complex (TCR and BCR) . CD5 crosslinking induces extracellular Ca++ mobilization, tyrosine phosphorylation of intracellular proteins and DAG production. Preliminary evidence shows protein associations with ZAP-70, p56lck, p59fyn, PC-PLC, etc. CD5 may serve as a dual receptor, giving either stimulatory or inhibitory signals depending both on the cell type and development stage. In thymocytes and B1a cells it seems to provide inhibitory signals, in peripheral mature T lymhocytes it acts as a costimulatory signal receptor. CD5 is the phenotypic marker of a B cell subpopulation involved in the production of autoreactive antibodies. Disease relevance: CD5 is a phenotypic marker for some B cell lymphoproliferative disorders (B-CLL, Hairy cell leukemia, etc.) . The CD5+ popuation is expanded in some autoimmune disorders (rheumatoid arthritis, etc.) . Herpes virus infections induce loss of CD5 expression in the expanded CD8+ human T cells.

Human

Stimulated human leukocytes

CD5

CRIS1

FC (QC tested)

APC

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

*Guarne A, Bravo J, Calvo J, Lozano F, Vives J, Fita I: Conformation of the hypervariable region L3 without the key proline residue. Protein Sci. 1996 Jan;5 (1) :167-9. , URL: https://www.ncbi.nlm.nih.gov/pubmed/8771210?dopt=A, *Leukocyte Typing III., McMichael A. J. et al. (Eds.), Oxford University Press (1987) ., URL: , *Arrizabalaga P, Mirapeix E, Darnell A, Torras A, Revert L: Cellular immunity analysis using monoclonal antibodies in human glomerulonephritis. Nephron. 1989;53 (1) :41-9., URL: https://www.ncbi.nlm.nih.gov/pubmed/2789343?dopt=A, *Alberola-Ila J, Places L, Cantrell DA, Vives J, Lozano F: Intracellular events involved in CD5-induced human T cell activation and proliferation. J Immunol. 1992 Mar 1;148 (5) :1287-93., URL: https://www.ncbi.nlm.nih.gov/pubmed/1371522?dopt=A

*Freedman AS, Freeman G, Whitman J, Segil J, Daley J, Levine H, Nadler LM: Expression and regulation of CD5 on in vitro activated human B cells. Eur J Immunol. 1989 May;19 (5) :849-55. , URL: https://www.ncbi.nlm.nih.gov/pubmed/2472277?dopt=A, *Raman C: CD5, an important regulator of lymphocyte selection and immune tolerance. Immunol Res. 2002;26 (1-3) :255-63., URL: https://pubmed.ncbi.nlm.nih.gov/12403363/

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

The mouse monoclonal antibody CRIS1 reacts with an extracellular epitope of CD5, a 67kDa single-chain transmembrane glycoprotein expressed on mature T lymphocytes, most of thymocytes and B lymphocytes subset (B-1a lymphocytes) .

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